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Flt-3 ligand (human), (recombinant)

 
ALX-201-800-0002 2 µg 149.00 USD
 
ALX-201-800-0010 10 µg 251.00 USD
 
ALX-201-800-0100 100 µg 887.00 USD
Do you need bulk/larger quantities?
 
  • Carrier-free

Product Specification

Alternative Name:FMS-related tyrosine kinase 3 ligand
 
MW:~17.6kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 155 amino acids.
 
UniProt ID:P49771
 
Formulation:Lyophilized from sterile filtered solution containing 10 mM sodium phosphate, 50 mM NaCl, pH 7.5.
 
Purity:≥95% (Reducing and Non-reducing SDS-PAGE)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent stimulation of the proliferation of human Aml5 cells which is typically observed at a concentration of 1-3 ng/mL.
 
Reconstitution:Reconstitute with sterile water at 0.1 mg/ml.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:FMS-related tyrosine kinase 3 ligand (Flt-3 ligand) is a growth factor important for the proliferation of hematopoietic cells. Flt-3 ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like tyrosine kinase-3 (Flt-3). Flt-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt-3 ligand can stimulate the proliferation of cells expressing mouse Flt-3 receptors.
 
Regulatory Status:RUO - Research Use Only
 
Flt-3 ligand (human), (recombinant) SDS-PAGE
Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human Flt-3 ligand has a predicted MW of 17.6 kDa.
Flt-3 ligand (human), (recombinant) image
Figure 2: Serial dilutions of Human FLT-3 Ligand, starting at 100 ng/mL, were added to OCI-AML5 cells. After 60 hours, proliferation can be measured and the linear portion of the curve can be used to calculate the ED50.
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Flt-3 ligand (human), (recombinant) SDS-PAGE Flt-3 ligand (human), (recombinant) image

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