| Alternative Name: | FMS-related tyrosine kinase 3 ligand |
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| MW: | ~17.6kDa |
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| Source: | Produced in E. coli. Non-glycosylated protein, containing 155 amino acids. |
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| UniProt ID: | P49771 |
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| Formulation: | Lyophilized from sterile filtered solution containing 10 mM sodium phosphate, 50 mM NaCl, pH 7.5. |
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| Purity: | ≥95% (Reducing and Non-reducing SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | The activity is determined by the dose-dependent stimulation of the proliferation of human Aml5 cells which is typically observed at a concentration of 1-3 ng/mL. |
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| Reconstitution: | Reconstitute with sterile water at 0.1 mg/ml. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | It is recommended that a carrier protein (0.1% BSA) is added for long term storage. |
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| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | FMS-related tyrosine kinase 3 ligand (Flt-3 ligand) is a growth factor important for the proliferation of hematopoietic cells. Flt-3 ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like tyrosine kinase-3 (Flt-3). Flt-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt-3 ligand can stimulate the proliferation of cells expressing mouse Flt-3 receptors. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human Flt-3 ligand has a predicted MW of 17.6 kDa.
Figure 2: Serial dilutions of Human FLT-3 Ligand, starting at 100 ng/mL, were added to OCI-AML5 cells. After 60 hours, proliferation can be measured and the linear portion of the curve can be used to calculate the ED50.
