A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection Nestin in rat cells and tissues
ENZ-GEN135-2000
2 ml
750.00 USD
AMPIVIEW™ Nestin (AS) Dig RNA Probes (rat) are digoxigenin-labeled RNA probes targeting nestin for in situ hybridization in tissues and cells. The probes are formulated in a buffered formamide solution with hybridization enhancers.
Nestin detection in rat brain tissue with AMPIVIEW™ Nestin (AS) Dig RNA probes (rat) (ENZ-GEN135) (brown) (left column) and no probes (no signal) (middle column). Ubiquitin (brown) detection was used as a positive control with AMPIVIEW™ Ubiquitin (AS) Dig RNA probes (ENZ-GEN125) (right column). Images have been captured in 4X, 20X and 40X magnification.
Nestin (brown) detection in rat brain tissue with AMPIVIEW™ Nestin (AS) Dig RNA probes (rat) (ENZ-GEN135), amplified with DIGX® anti-digoxigenin linker, detected with POLYVIEW® PLUS HRP combined with HIGHDEF® DAB chromogen and counterstained with HIGHDEF® hematoxylin.
Nestin (no signal) detection in rat brain tissue with no probes, amplified with DIGX® anti-digoxigenin linker, detected with POLYVIEW® PLUS HRP combined with HIGHDEF® DAB chromogen and counterstained with HIGHDEF® hematoxylin.
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Product Details
Applications:
ISH (in situ hybridization)
Application Notes:
AMPIVIEW™ Nestin (AS) Dig RNA probes have been designed to target rat neuroepithelial stem cell proteins (Nestin) in tissues and cells. Nestin is a type IV intermediate filament protein expressed in normal and diseased cells in different tissues and organs. Nestin is expressed by many types of cells during development. In adult cells, Nestin is reinduced during pathological situations, such as central nervous system injury and during regeneration of injured muscle tissue. Nestin is also used as a biomarker for neural stem cells and angiogenesis of proliferating endothelial cells in colorectal cancer.
AMPIVIEW™ Nestin (AS) Dig RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo's DIGX® anti-digoxigenin linker and nanopolymner-based detection systems, POLYVIEW® PLUS, combined with HIGHDEF® chromogens and counterstain to produce clear results that can be visualized with a light microscope.
Species reactivity:
Rat
Contents:
2 x 1 mL AMPIVIEW™ Nestin (AS) Dig RNA Probes (Rat) (2µg/mL)
1 x 2 mL AMPIVIEW™ Hybridization Buffer (1X)
Use/Stability:
Aliquot and store at -20°C or -80°C. Under these conditions, products are stable until its expiration dates.
Handling:
Allow contents to warm up to room temperature prior to use.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Technical Info/Product Notes:
Neuroepithelial stem cell protein (NESTIN) is a type IV intermediate filament (IF) protein expressed in normal and diseased cells in different tissues and organs. Among neural cells in the developing and adult CNS, nestin expression is thought to occur exclusively in uncommitted neural progenitor cells (NPCs). After NPCs differentiate, nestin expression typically is replaced by the expression of neuronal or glial specific markers.
AMPIVIEW™ Nestin (AS) Dig RNA Probes (Rat) are optimized for detection of nestin RNA or RNA/DNA in FFPE tissues or cells with DIGX® anti-digoxigenin linker, POLYVIEW® PLUS detection reagents combined with HIGHDEF® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW™ Hybridization Buffer (1X), included in this set.