HIGHDEF® red IHC chromogen (AP, plus) is intended to be used in conjuction with alkaline phosphatase (AP)-based immunostaining or in situ hybridization systems that run on automated staining instruments as well as manual use.
Immunohistochemistry analysis: Formalin fixed paraffin embedded squamous cell carcinoma human tissue stained with a CK 5/6 antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) (Prod. no. ADI-950-141) produces a brilliant red color.
Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF® IHC chromogen substrate (DAB, HC) (Prod. no. ADI-950-211, brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF® blue IHC chromogen (AP) (Prod. no. ADI-950-150, blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) (Prod. no. ADI-950-141, red), high molecular weight cytokeratin labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, yellow), and methyl green nuclear counterstain.
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Product Details
| Applications: | IHC
ISH (in situ hybridization)
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| Contents: | HIGHDEF® red IHC chromogen (AP, plus) substrate buffer and chromogen, Empty mixing bottle |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Technical Info/Product Notes: | Traditional formulations for red alkaline phosphatase chromogens are unstable upon mixing. This can be problematic for use in automated slide strainers. The HIGHDEF® red IHC chromogen (AP, plus) has been formulated for on-slide and on-board mixing for any autostainer. Both components are stable and can be loaded at the beginning of the staining run, for walk away convenience. It produces a red color, ranging from pink to brilliant dark red and is insoluble in organic solvents; therefore sections can be dehydrated in alcohol, cleared in xylene (or a xylene-substitute), and permanently mounted. This chromogen substrate system may be used for both automation and manual use. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Inhibition of mitochondria ATP synthase suppresses prostate cancer growth through reduced insulin‐like growth factor‐1 secretion by prostate stromal cells: T. Ohishi, et al.; Int. J. Cancer
146, 3474 (2020),
Abstract;
CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy: W. Wang, et al.; Nature
569, 270 (2019),
Abstract;
Expression of programmed cell death ligand 1 is associated with poor overall survival in patients with diffuse large B-cell lymphoma: J. Kiyasu, et al.; Blood
126, 2193 (2015),
Application(s): IHC using FFPE tissue,
Abstract;
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