HIGHDEF® Hematoxylin is intended for use in the histologic demonstration of nuclear staining. This staining technique is sometimes used to make the critical distinction between a normal nucleus and an abnormal one.
Product Details
Formulation:
Enhancers added to improve signal.
Applications:
IHC ISH (in situ hybridization)
Shipping:
Ambient Temperature
Long Term Storage:
Ambient
Technical Info/Product Notes:
Hematoxylin is one of the most common staining compounds used in histology.
The protocols for specific applications can vary. These include, but are not limited to: fixation, heat-retrieval methods, incubation times, tissue section thickness, and detection kit used. Due to the superior sensitivity of these unique reagents, the recommended incubation times and titers listed are not applicable to other detection systems, as results may vary. Ultimately, it is the responsibility of the investigator to determine optimal conditions. These products are tools that can be used for interpretation of morphological findings in conjunction with other diagnostic tests and pertinent clinical data by a qualified pathologist.
Protocol:
Deparaffinize and rehydrate tissue.
Apply enough HIGHDEF® Hematoxylin to completely cover the tissue (usually about 150 μL) and incubate for 2-10 minutes, according to desired intensity.
Rinse in deionized water for 20 to 30 seconds.
Follow suggested dehydration and mounting procedures based on the chromogen used.
Regulatory Status:
RUO - Research Use Only
Enzo's hematoxylin allows for clearer staining and less background than a leading competitor. Both slides were stained with Enzo's POLYVIEW® PLUS HRP-DAB Kit, but the competitor's hematoxylin was used on the slide on the right, showing more background and a less clear result.
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Product Literature References
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Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions: M. Leite, et al.; Cells 9, 513 (2020), Abstract; Full Text
Afadin downregulation by Helicobacter pylori induces epithelial to mesenchymal transition in gastric cells: M.S. Marques, et al.; Front. Microbiol. 9, 2712 (2018), Abstract; Full Text
Protective effect of Azadirachta indica and vitamin E against arsenic acid-induced genotoxicity and apoptosis in rats: A.A. Oyagbemi, et al.; J. Diet. Suppl. 15, 251 (2018), Abstract;
Cobalt chloride exposure dose-dependently induced hepatotoxicity through enhancement of cyclooxygenase-2 (COX-2)/B-cell associated protein X (BAX) signaling and genotoxicity in Wistar rats: O.V. Awoyemi, et al.; Environ. Toxicol. 32, 1899 (2017), Abstract;
Kolaviron attenuated arsenic acid induced-cardiorenal dysfunction via regulation of ROS, C-reactive proteins (CRP), cardiac troponin I (CTnI) and BCL2: A.A. Oyagbemi, et al.; J. Tradit. Complement Med. 8, 396 (2017), Abstract; Full Text
