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HIGHDEF® yellow IHC chromogen (HRP)

Unique high definition yellow IHC chromogen for HRP-activated development.
 
ADI-950-170-0030 30 ml 215.00 USD
Do you need bulk/larger quantities?
 
HIGHDEF® yellow IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridization (ISH) systems. With at least 1 day working stability, this substrate/chromogen system can be used in any automation system as well as manual use.
HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil multiplex 2
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with CD8 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD68 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD163 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. Image courtesy from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.
HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil multiplex
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with FOXP3 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD3 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD8 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. Image used with permission from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.
HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil CK
Immunohistochemistry analysis: Formalin fixed paraffin embedded human tonsil stained with a high molecular weight CK antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170) produces a distinct golden yellow color.
HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil Ki67
Immunohistochemical analysis: Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.
HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil
Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF® IHC chromogen substrate (DAB, HC) (Prod. no. ADI-950-211, brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF® blue IHC chromogen (AP) (Prod. no. ADI-950-150, blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) (Prod. no. ADI-950-141, red), high molecular weight cytokeratin labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, yellow), and methyl green nuclear counterstain.
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HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil multiplex 2 HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil multiplex HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil CK HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil Ki67 HIGHDEF yellow IHC chromogen (HRP) IHC human tonsil

Product Details

Applications:IHC
ISH (in situ hybridization)
 
Contents:HIGHDEF® yellow IHC chromogen (HRP) substrate buffer and chromogen, Empty mixing bottle
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Application Note
Three-color IHC Staining On Free-floating Sections From Human Brain
Tumor Phenotyping and Immune Cell Regulation using Multiplex IHC
 
Technical Info/Product Notes:The HIGHDEF® yellow IHC chromogen (HRP) is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing horseradish peroxidase. It produces a distinct bright yellow that can easily be distinguished from other stains.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Inhibition of DNMT3B and PI3K/AKT/mTOR and ERK Pathways as a Novel Mechanism of Volasertib on Hypomethylating Agent-Resistant Cells: E.J. Choi, et al.; Biomol. Ther. 31, 319 (2023), Abstract;
The differential expression of toll like receptors and RIG-1 in the placenta of neonates with in utero infections: M. Rice, et al.; Ann. Diagn. Pathol. 62, 152080 (2023), Abstract;
Inhibition of mitochondria ATP synthase suppresses prostate cancer growth through reduced insulin‐like growth factor‐1 secretion by prostate stromal cells: T. Ohishi, et al.; Int. J. Cancer 146, 3474 (2020), Abstract;
Intraepithelial T cells diverge by intestinal location as pigs age: J.E. Wiarda, et al.; Front. Immunol. 11, 1139 (2020), Abstract; Full Text
CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy: W. Wang, et al.; Nature 569, 270 (2019), Abstract;
Reciprocal nerve staining (RNS) for the concurrent detection of choline acetyltransferase and myelin basic protein on paraffin-embedded sections: A. Merolli, et al.; J. Neurosci. Methods 311, 235 (2019), Abstract;
Retinoblastoma protein represses E2F3 to maintain Sertoli cell quiescence in mouse testis: E. Rotgers, et al.; J. Cell. Sci. 132, jcs229849 (2019), Abstract;
Inhibition of Nox4-dependent ROS signaling attenuates prostate fibroblast activation and abrogates stromal-mediated protumorigenic interactions: N. Sampson, et al.; Int. J. Cancer 143, 383 (2018), Application(s): IHC using FFPE prostate tumor sections, Abstract; Full Text
Rationale and pre-clinical evidences for the use of autologous cartilage micrografts in cartilage repair: M. Vigano, et al.; J. Orthop. Surg. Res. 13, 279 (2018), Abstract; Full Text
APC/C is essential for hematopoiesis and impaired in aplastic anemia: J. Wang, et al.; Oncotarget 8, 63360 (2017), Application(s): IHC using FFPE murine bone marrow sections , Abstract; Full Text
The stimulation of macrophages with TLR ligands supports increased IL-19 expression in inflammatory bowel disease patients and in colitis models: A. Steinert, et al.; J. Immunol. 199, 2570 (2017), Abstract;
Saturated and unsaturated fatty acids differently modulate colonic goblet cells in vitro and in rat pups: B. Benoit, et al.; J. Nutr. 145, 1754 (2015), Abstract;

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