HIGHDEF® yellow IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridization (ISH) systems. With at least 1 day working stability, this substrate/chromogen system can be used in any automation system as well as manual use.
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with CD8 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD68 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD163 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. Image courtesy from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with FOXP3 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD3 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD8 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. Image used with permission from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.
Immunohistochemistry analysis: Formalin fixed paraffin embedded human tonsil stained with a high molecular weight CK antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170) produces a distinct golden yellow color.
Immunohistochemical analysis: Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.
Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF® IHC chromogen substrate (DAB, HC) (Prod. no. ADI-950-211, brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF® blue IHC chromogen (AP) (Prod. no. ADI-950-150, blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) (Prod. no. ADI-950-141, red), high molecular weight cytokeratin labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, yellow), and methyl green nuclear counterstain.
The HIGHDEF® yellow IHC chromogen (HRP) is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing horseradish peroxidase. It produces a distinct bright yellow that can easily be distinguished from other stains.
Regulatory Status:
RUO - Research Use Only
Product Literature References
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The differential expression of toll like receptors and RIG-1 in the placenta of neonates with in utero infections: M. Rice, et al.; Ann. Diagn. Pathol. 62, 152080 (2023), Abstract;
Inhibition of mitochondria ATP synthase suppresses prostate cancer growth through reduced insulin‐like growth factor‐1 secretion by prostate stromal cells: T. Ohishi, et al.; Int. J. Cancer 146, 3474 (2020), Abstract;
Intraepithelial T cells diverge by intestinal location as pigs age: J.E. Wiarda, et al.; Front. Immunol. 11, 1139 (2020), Abstract; Full Text
CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy: W. Wang, et al.; Nature 569, 270 (2019), Abstract;
Reciprocal nerve staining (RNS) for the concurrent detection of choline acetyltransferase and myelin basic protein on paraffin-embedded sections: A. Merolli, et al.; J. Neurosci. Methods 311, 235 (2019), Abstract;
Retinoblastoma protein represses E2F3 to maintain Sertoli cell quiescence in mouse testis: E. Rotgers, et al.; J. Cell. Sci. 132, jcs229849 (2019), Abstract;
Inhibition of Nox4-dependent ROS signaling attenuates prostate fibroblast activation and abrogates stromal-mediated protumorigenic interactions: N. Sampson, et al.; Int. J. Cancer 143, 383 (2018), Application(s): IHC using FFPE prostate tumor sections, Abstract; Full Text
Rationale and pre-clinical evidences for the use of autologous cartilage micrografts in cartilage repair: M. Vigano, et al.; J. Orthop. Surg. Res. 13, 279 (2018), Abstract; Full Text
APC/C is essential for hematopoiesis and impaired in aplastic anemia: J. Wang, et al.; Oncotarget 8, 63360 (2017), Application(s): IHC using FFPE murine bone marrow sections , Abstract; Full Text
The stimulation of macrophages with TLR ligands supports increased IL-19 expression in inflammatory bowel disease patients and in colitis models: A. Steinert, et al.; J. Immunol. 199, 2570 (2017), Abstract;
Saturated and unsaturated fatty acids differently modulate colonic goblet cells in vitro and in rat pups: B. Benoit, et al.; J. Nutr. 145, 1754 (2015), Abstract;
