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HIGHDEF® black IHC chromogen (HRP)

Unique high definition black IHC chromogen for HRP-activated development.
 
ADI-950-171-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 
Available from stock, Ready for shipment.
HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridization systems. With at least 1 day working stability, this substrate/chromogen system can be used in any automation system as well as manual use.
HIGHDEF black IHC chromogen (HRP) IHC human tonsil multiplex
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with FOXP3 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD3 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD8 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. (Image courtesy from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.)
HIGHDEF black IHC chromogen (HRP) IHC human tonsil multiplex 2
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with CD8 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD68 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD163 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. Image courtesy from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.
HIGHDEF black IHC chromogen (HRP) IHC human tonsil HMWCK
Immunohistochemistry analysis: Formalin fixed paraffin embedded human tonsil stained with a high molecular weight cytokeratin antibody labeled and HIGHDEF® black IHC chromogen (HRP) (Prod. no. ADI-950-171) produces a sharply contrasting ebony black color.
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HIGHDEF black IHC chromogen (HRP) IHC human tonsil multiplex HIGHDEF black IHC chromogen (HRP) IHC human tonsil multiplex 2 HIGHDEF black IHC chromogen (HRP) IHC human tonsil HMWCK

Product Details

Applications:IHC
ISH (in situ hybridization)
 
Contents:HIGHDEF® black IHC chromogen (HRP) substrate buffer and chromogen, Empty mixing bottle
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Application Note
Tumor Phenotyping and Immune Cell Regulation using Multiplex IHC
 
Technical Info/Product Notes:The HIGHDEF® black IHC chromogen (HRP) is a substrate-chromogen system designed to be used for either immunohistochemistry (IHC) or ISH when utilizing horseradish peroxidase. HIGHDEF® black IHC chromogen (HRP) produces a sharply contrasting ebony black color that can easily be distinguished from other stains.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Reciprocal nerve staining (RNS) for the concurrent detection of choline acetyltransferase and myelin basic protein on paraffin-embedded sections: A. Merolli, et al.; J. Neurosci. Methods 311, 235 (2019), Abstract;

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