HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridization systems. With at least 1 day working stability, this substrate/chromogen system can be used in any automation system as well as manual use.
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with FOXP3 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD3 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD8 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. (Image courtesy from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.)
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with CD8 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD68 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD163 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. Image courtesy from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.
Immunohistochemistry analysis: Formalin fixed paraffin embedded human tonsil stained with a high molecular weight cytokeratin antibody labeled and HIGHDEF® black IHC chromogen (HRP) (Prod. no. ADI-950-171) produces a sharply contrasting ebony black color.
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Product Details
Applications:
IHC ISH (in situ hybridization)
Contents:
HIGHDEF® black IHC chromogen (HRP) substrate buffer and chromogen, Empty mixing bottle
The HIGHDEF® black IHC chromogen (HRP) is a substrate-chromogen system designed to be used for either immunohistochemistry (IHC) or ISH when utilizing horseradish peroxidase. HIGHDEF® black IHC chromogen (HRP) produces a sharply contrasting ebony black color that can easily be distinguished from other stains.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Overcoming resistance to αPD-1 of MMR-deficient tumors with high tumor-induced neutrophils levels by combination of αCTLA-4 and αPD-1 blockers: L.N. Bral, et al.; J. Immunother. Cancer 10, e005059 (2022), Abstract;
Reciprocal nerve staining (RNS) for the concurrent detection of choline acetyltransferase and myelin basic protein on paraffin-embedded sections: A. Merolli, et al.; J. Neurosci. Methods 311, 235 (2019), Abstract;
