HIGHDEF® red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridization systems.
Immunohistochemical analysis: Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.
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Product Details
| Applications: | IHC
ISH (in situ hybridization)
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| Contents: | HIGHDEF® red IHC chromogen (AP) substrate buffer and chromogen, Empty mixing bottle |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Technical Info/Product Notes: | The HIGHDEF® red IHC chromogen (AP) is a substrate-chromogen system designed to be used for either IHC or ISH when using alkaline phosphatase detection. The system produces a brilliant dark red color and is insoluble in organic solvents; therefore, sections can be dehydrated in alcohol, cleared in xylene (or a xylene-substitute), and permanently mounted. This chromogen substrate system may be used for both automation and manual use. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Application(s): Multiplex IHC staining on human cardiac tissue,
Abstract;
Downregulation of renal tubular Wnt/β-catenin signaling by Dickkopf-3 induces tubular cell death in proteinuric nephropathy: D.W. Wong, et al.; Cell Death Dis.
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Application(s): Immunohistochemical staining,
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Molecular signals involved in human B cell migration into the retina: in vitro investigation of ICAM-1, VCAM-1, and CXCL13: A.S. Bharadwaj, et al.; Ocul. Immunol. Inflamm.
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