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HIGHDEF® red IHC chromogen (AP)

High definition IHC chromogen producing brilliant red color for AP-activated development.
 
ADI-950-140-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 
Available from stock, Ready for shipment.
HIGHDEF® red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridization systems.
HIGHDEF® red IHC chromogen (AP) Immunohistochemistry
Immunohistochemical analysis: Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.
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HIGHDEF® red IHC chromogen (AP) Immunohistochemistry

Product Details

Applications:IHC
ISH (in situ hybridization)
 
Contents:HIGHDEF® red IHC chromogen (AP) substrate buffer and chromogen, Empty mixing bottle
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:The HIGHDEF® red IHC chromogen (AP) is a substrate-chromogen system designed to be used for either IHC or ISH when using alkaline phosphatase detection. The system produces a brilliant dark red color and is insoluble in organic solvents; therefore, sections can be dehydrated in alcohol, cleared in xylene (or a xylene-substitute), and permanently mounted. This chromogen substrate system may be used for both automation and manual use.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Exploring human splenic red pulp vasculature in virtual reality: details of sheathed capillaries and the open capillary network: B.S. Steiniger, et al.; Histochem. Cell Biol. 155, 341 (2021), Abstract; Full Text
B cells are associated with survival and immunotherapy response in sarcoma: F. Petitprez, et al.; Nature 577, 556 (2020), Abstract;
Cannabinoid receptor 1 knockout alleviates hepatic steatosis by downregulating perilipin 2: K. Irungbam, et al.; Lab. Invest. 100, 454 (2020), Abstract; Full Text
Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice: K. Irungbam, et al.; Lab. Invest. 100, 1411 (2020), Abstract; Full Text
CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy: W. Wang, et al.; Nature 569, 270 (2019), Abstract; Full Text
Nuclear immunoreactivity of BLM-s, a proapoptotic BCL-2 family member, is specifically detected in salivary adenoid cystic carcinoma: M.S. Tsai, et al.; Hum. Pathol. 84, 81 (2019), Abstract;
Preparation and characterization of gellan gum/glucosamine/clioquinol film as oral cancer treatment patch: W. Tsai, et al.; Mater. Sci. Eng. C Mater. Biol. Appl. 82, 317 (2018), Abstract;
Prevalence of polyreactive innate clones among graft-infiltrating B cells in human cardiac allograft vasculopathy: D. Chatterjee, et al.; J. Heart Lung Transplant. 37, 385 (2018), Application(s): Multiplex IHC staining on human cardiac tissue, Abstract;
Downregulation of renal tubular Wnt/β-catenin signaling by Dickkopf-3 induces tubular cell death in proteinuric nephropathy: D.W. Wong, et al.; Cell Death Dis. 7, e2155 (2016), Application(s): Immunohistochemical staining, Abstract;
Molecular signals involved in human B cell migration into the retina: in vitro investigation of ICAM-1, VCAM-1, and CXCL13: A.S. Bharadwaj, et al.; Ocul. Immunol. Inflamm. 5, 1 (2016), Abstract;

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