Highest sensitivity, complete staining kit for both IHC and ISH
POLYVIEW® PLUS reagents' biotin free nanopolymer detection circumvents endogenous background while providing specific antigen localization
HIGHDEF® DAB (Brown) HRP provides high intensity color development to deliver sharp, crisp staining
Compatible with both manual and automated platforms
AMPIVIEW™ POLYVIEW® PLUS HRP-DAB (Anti-Mouse) Kit is intended for AMPIVIEW™ RNA probes ISH staining. The kit is a nanopolymer detection system, combined with DIGX® mouse anti-digoxigenin, is suitable for the detection of nucleic acid in formalin-fixed paraffin-embedded (FFPE) tissue specimens.
HPV 16/18 detected (brown) in infected cervical tissue with AMPIVIEW HPV 16/18 RNA probes and POLYVIEW (anti-mouse) HRP/DAB detection kit.
HPV 16/18 not detected in normal cervical tissue with AMPIVIEW 16/18 RNA probes and POLYVIEW (anti-m) HRP/DAB detection kit.
The POLYVIEW® PLUS HRP-DAB (Anti-Mouse) Kit contains POLYVIEW® PLUS HRP (Anti-Mouse) Reagent that has been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyper-labeling technology in ready-to-use format. This ensures consistent and reproducible immunodetection of mouse antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. POLYVIEW® PLUS HRP (Anti-Mouse) Reagent allows faster staining procedures as compared to traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background.
Regulatory Status:
RUO - Research Use Only
Product Literature References
False-positive results in diagnostic immunohistochemistry are related to horseradish peroxidase conjugate in commercially available assays: Gerard J. Nuovo; Annals of Diagnostic Pathology 25, 54-59 (2016), Abstract;
