AMPIVIEW™ EGFR (AS) Dig RNA Probes are digoxigenin-labeled RNA probes targeting EGFR nucleic acid (DNA/RNA or RNA) for in situ hybridization in tissues and cells. The probes are formulated in a buffered formamide solution with hybridization enhancers.
EGFR (red) detected in prostate hyperplasia tissue specimen with AMPIVIEW™ EGFR Dig RNA probes, DIGX® rabbit anti-digoxigenin linker (ENZ-ABS303), POLYVIEW® PLUS AP (anti-rabbit) (ENZ-ACC110) combined with HIGHDEF® Red Chromogen/Substrate and Hematoxylin counterstain (ENZ-ACC106).
AMPIVIEW™ NSP Dig RNA probes (no signal) detected in prostate hyperplasia tissue specimen with DIGX® rabbit anti-digoxigenin linker (ENZ-ABS303), POLYVIEW® PLUS AP (anti-rabbit) (ENZ-ACC110), combined with HIGHDEF® Red Chromogen/Substrate and Hematoxylin counterstain (ENZ-ACC106). AMPIVIEW™ NSP Dig RNA probes are negative control probes.
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Product Details
Applications:
ISH (in situ hybridization)
Application Notes:
AMPIVIEW™ EGFR (AS) Dig RNA probes have been designed to target the EGFR or HER-1 DNA and RNA in tissues and cells. AMPIVIEW™ EGFR (AS) Dig RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo's DIGX® anti-digoxigenin linker and nanopolymner-based detection systems, POLYVIEW® PLUS, combined with HIGHDEF® chromogens and counterstain to produce clear results that can be visualized with a light microscope.
Species reactivity:
Human
Contents:
2 x 1 mL AMPIVIEW™ EGFR (AS) Dig RNA Probes (2μg/mL)
1 x 2 mL AMPIVIEW™ Hybridization Buffer (1X)
Use/Stability:
Aliquot and store at -20ºC or -80ºC. Under these conditions, products are stable until its expiration dates.
Handling:
Allow contents to warm up to room temperature prior to use.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Technical Info/Product Notes:
AMPIVIEW™ EGFR (AS) Dig RNA Probes are optimized for detection of EGFR RNA or RNA/DNA in FFPE tissues or cells with DIGX® anti-digoxigenin linker, POLYVIEW® PLUS detection reagents combined with HIGHDEF® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW™ Hybridization Buffer (1X), included in this set.
