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Permanent, high stability mounting medium for high definition visualization of IHC.
ADI-950-261-0030 30 ml 80.00 USD
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  • Aqueous-based permanent mounting medium designed for chromogenic use and ideal for long-term storage of processed tissue
  • Flexible formulation optimized for AEC, DAB, Fast Red, BCIP/NBT, and BCIP/INT
  • High definition visualization for IHC, IF, ISH, FISH
HIGHDEF® IHC mount is an aqueous-based permanent mounting medium designed for use with chromogenic labeling. Use for mounting of tissue specimens stained with peroxidase and alkaline phosphatase-based systems and various fluorescent dyes.

Product Details

Formulation:Liquid. Mounting medium containing <0.1% sodium azide.
Applications:IF, IHC
ISH (in situ hybridization)
Shipping:Ambient Temperature
Long Term Storage:Ambient
Scientific Background:AEC and Fast Red are the most commonly used red chromogens for peroxidase and alkaline phosphatase based immunostaining systems. Slides stained with these chromogens cannot be stored permanently because of the lack of availability of an aqueous permanent mounting medium with high refractive index. HIGHDEF® IHC mount is designed to fill in these limitations and it is an aqueous mounting medium with a very high refractive index. It allows permanent storage of tissue specimens without fading of the chromogens. Because of its superior refractive index, tissues mounted in this medium look like dehydrated specimens. No coverslipping is required. If coverslipping is required, dried HIGHDEF® mount can be post mounted using an organic-based mounting medium. Advantages of HIGHDEF® mount are numerous: no coverslip is required, there is no exposure to organic fumes, it allows permanent storage of slides, and it provides a high resolution of tissue specimens. HIGHDEF® IHC mount is compatible with chromogens like AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT, and fluorescent dyes like FITC and phycobiliproteins. HIGHDEF® IHC mount's high pH ensures increased stability of fluorescence.

Application Note
Tumor Phenotyping and Immune Cell Regulation using Multiplex IHC
Technical Info/Product Notes:Interpretation of the results will be the sole responsibility of the user.
Protocol:Mounting Procedure
  1. Remove red tip from the bottle and cut the tip off with a sharp razor or scissors. This will help achieve smooth flow of HIGHDEF® IHC mount and prevent formation of tiny bubbles.
  2. Place the bottle upside down in a container before use. This will help clear the HIGHDEF® IHC mount bubbles.
  3. Blot excess water from the slide without letting tissue specimen dry. Make sure tissue is wet prior to mounting.
  4. Apply 2-3 drops of Mount on the tissue section. Gently rotate the slide to make a thin layer of Mount. Make sure the tissue is completely covered.
  5. Do not apply coverslip. Place slides in an oven preheated at 70°C. Make sure the oven is completely horizontal. Drying time will range from 10-20 minutes depending on the amount of Mount applied.
  6. Take slides out, let them cool, and look at them under the microscope.
  7. If heating is not desired for specimens mounted with fluorescent dyes, place a cover slip on the liquid HIGHDEF® IHC mount and let sit at room temperature for 45 minutes to 1 hour to allow Mount to dry.
  8. Seal coverlisp edges with nail polish for long term storage.
Post Mounting: Use of oil lens requires post mounting. Once Mount is thoroughly dry and hard, apply an organic-based mounting media like E-Z mount, Protexx, Permount, or Shandon-Mount. Then apply a coverslip. Mount has a refrective index very close to organic-based mounting media.
Removal of Mount: Mount is an aqueous-based mounting medium that can easily be removed by soaking the slides in de-ionized water. Place the slide in a beaker full of de-ionized water on a magnetic stirrer. Leave the slide for few hours or overnight with gentle stirring for complete removal of dry Mount
Regulatory Status:RUO - Research Use Only

Product Literature References

T-cell mediated targeted delivery of anti-PD-L1 nanobody overcomes poor antibody penetration and improves PD-L1 blocking at the tumor site: P.F. Petit, et al.; Cancer Immunol. Res. 1158, 2326 (2022), Abstract;
Morphine-conditioned placebo analgesia in female and male rats with chronic neuropathic pain: c-fos expression in the rostral ventromedial medulla: D.C. Boorman, et al.; Neuroscience 457, 51 (2021), Abstract;
SPON1 can reduce amyloid beta and reverse cognitive impairment and memory dysfunction in Alzheimer's disease mouse model: S.Y. Park, et al.; Cells 9, 1275 (2020), Abstract;
Tryptophan 2, 3-dioxygenase expression identified in human hepatocellular carcinoma cells and in intratumoral pericytes of most cancers: D. Hoffmann, et al.; Cancer Immunol. Lett. 8, 19 (2020), Abstract;
Tryptophan 2, 3-dioxygenase expression identified in murine decidual stromal cells is not essential for feto-maternal tolerance: D. Hoffmann, et al.; Front. Immunol. 11, 601759 (2020), Abstract; Full Text
Peripheral nerve injury attenuates stress-induced Fos-family expression in the locus coeruleus of male Sprague-Dawley rats: D.C. Boorman, et al.; Brain Res. 1719, 253 (2019), Abstract;

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