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SAVIEW® PLUS AP Reagent

High sensitivity, low background, streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
 
ENZ-ACC111-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 
Available from stock, Ready for shipment.
  • Ready-to-use reagent
  • Streptavidin-based detection with reduced carbohydrate and biotin background
  • Suitable for both manual and automated platforms
  • Streptavidin Blocker/Diluent further reduces background (sold separately)
  • 5-10X more sensitive than avidin based detection
  • Customize with HIGHDEF® chromogens of your choice (sold separately)
SAVIEW® PLUS AP Reagent is a ready-to-use streptavidin-based nanopolymer detection reagent suitable for immunohistochemical detection of antigens in formalin-fixed paraffin-embedded (FFPE) tissues and frozen sections. The detection reagent may also be used with blood smears, cytosmears, and cell preparations. In addition, the SAVIEW® PLUS AP Reagent can be used for in situ hybridization detection of biotinylated DNA/RNA probes.
SAVIEW® PLUS AP Reagent 2
SAVIEW® PLUS AP (Enzo Life Sciences) was used to perform ISH: Manual method. Sections were stained with HIGHDEF® Green AP Chromogen/Substrate and counterstained with HIGHDEF® Hematoxylin. Images acquired under 10X objective.
SAVIEW® PLUS AP Reagent Immunohistochemistry
Mouse anti-CD44 and SAVIEW® PLUS AP (Enzo Life Sciences) were used to perform IHC: Manual method. Sections were stained with HIGHDEF® Green AP Chromogen/Substrate and counterstained with HIGHDEF® Hematoxylin. Images acquired under 10X objective.
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SAVIEW® PLUS AP Reagent 2 SAVIEW® PLUS AP Reagent Immunohistochemistry

Product Details

Assay Time:< 2 hours
 
Applications:IHC
ISH (in situ hybridization)
 
Application Notes:IHC
ISH (in situ hybridization)
 
Quantity:150 tests × 150 µL (22.5 mL total volume)
 
Handling:Do not freeze.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:The SAVIEW® PLUS AP Reagent has been developed by labeling streptavidin molecules with a nanopolymer of alkaline phosphatase (AP) using proprietary technology. This ensures consistent and reproducible detection of biotinylated primary/secondary antibodies or biotinylated nucleic acid probes on tissue sections.

The Streptavidin Blocker/Diluent (ENZ-ACC109) further reduces background and is suitable for diluting the SAVIEW® PLUS AP Reagent.
 
Regulatory Status:RUO - Research Use Only
 

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Do you need bulk/larger quantities?
 

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Streptavidin Blocker/Diluent 

Ready-to-use blocking buffer and diluent, for use with SAVIEW® PLUS detection reagents
IHC, ISH (in situ hybridization) | Print as PDF
 
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Do you need bulk/larger quantities?
 

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