The IHC PAP pen provides a hydrophobic barrier when a circle is drawn around a specimen on a glass slide.
Product Details
| Applications: | IF, IHC
ISH (in situ hybridization)
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | Ambient |
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| Use/Stability: | Always keep the pen tightly capped and store horizontally. Press down on the tip when the pen starts to dry out. |
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| Technical Info/Product Notes: | The IHC PAP pen provides a thin, film-like water repellent barrier when a circle is drawn around a specimen on a glass slide. This barrier creates the proper surface tension to hold antibody solution within the target area on the slide. This avoids repeated wipeout around the specimen during staining procedures. Since the reagent is guarded by the hydrophobic circle around the tissue, it minimizes the undesirable dilution of the reagents with the wash buffer. This pen can be used for PAP, ABC, immunofluorescence method, frozen sections, and in situ hybridization procedures. Two specimens separated by two circles can be applied to the same slide.
Interpretation of the results will be the sole responsibility of the user.
The pen is applicable to microwave staining and in-situ hybridization. Circles made by the pen are heat resistant up to 120°C, ready to be used directly on wet slides, insoluble in alcohol and acetone, and withstand rehydration and dehydration steps in alcohol. If desired, circles can be removed by xylene after the staining procedure is complete. |
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| Protocol: | Preparation before use: Before use, hold the pen point up and press it with your fingertip to take blocked air out from the pen barrel to prevent overflow.
Procedure: To start, press the tip of the pen straight down until fluid starts soaking the tip and then draw a circle around the specimen. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Synergistic antidepressant-like effect of n-3 polyunsaturated fatty acids and probiotics through the brain-gut axis in rats exposed to chronic mild stress: H. Cho, et al.; J. Nutr. Biochem.
116, 109326 (2023),
Abstract;
In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos: J. Wang, et al.; J. Vis. Exp.
3, 3791 (2022),
Abstract;
Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells: V. Manna, et al.; STAR Protoc.
2, 100782 (2021),
Abstract;
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