A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection lncRNA NORAD in mouse or rat cells and tissues
ENZ-GEN178-2000
2 ml
750.00 USD
AMPIVIEW™ NORAD (AS) Dig RNA Probes (mouse) are digoxigenin-labeled RNA probes targeting NORAD lncRNA for in situ hybridization in tissues and cells. The probes are formulated in a buffered formamide solution with hybridization enhancers.
NORAD (red) detection in rat brain tissue specimen with AMPIVIEW™ NORAD (AS) Dig RNA probes amplified with DIGX® rabbit anti-digoxigenin linker (ENZ-ABS303), detected with POLYVIEW® PLUS AP (anti-rabbit) (ENZ-ACC110) combined with HIGHDEF® Red AP Substrate/Chromogen and hematoxylin.
NORAD detection in rat brain tissue specimen AMPIVIEW™ NSP-Dig RNA probes (negative control), amplified with DIGX® rabbit anti-digoxigenin linker (ENZ-ABS303), detected with POLYVIEW® PLUS AP (anti-rabbit) (ENZ-ACC110) combined with HIGHDEF® Red AP Substrate/Chromogen and hematoxylin.
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Product Details
Applications:
ISH (in situ hybridization)
Application Notes:
AMPIVIEW™ NORAD (AS) Dig RNA probes have been designed to target mouse or rat non-coding RNA activated by DNA damage (NORAD) in tissues and cells. NORAD is a highly abundant, highly conserved mammalian long non-coding RNA (lncRNA) that is induced after DNA damage. AMPIVIEW™ NORAD (AS) Dig RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo's DIGX® anti-digoxigenin linker and nanopolymner-based detection systems, POLYVIEW® PLUS, combined with HIGHDEF® chromogens and counterstain to produce clear results that can be visualized with a light microscope.
Species reactivity:
Mouse, Rat
Contents:
2 x 1 mL AMPIVIEW™ NORAD (AS) Dig RNA Probes (2µg/mL)
1 x 2 mL AMPIVIEW™ Hybridization Buffer (1X)
Use/Stability:
Aliquot and store at -20°C or -80°C. Under these conditions, products are stable until its expiration dates.
Handling:
Allow contents to warm up to room temperature prior to use.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Technical Info/Product Notes:
NORAD is a long non-coding RNA that is induced after DNA damage. LncRNAs are identified as critical regulators in cerebral ischemia/reperfusion injury and are known to play an important role in the process of aging mammals.
AMPIVIEW™ NORAD (AS) Dig RNA Probes are optimized for detection of NORAD RNA or RNA/DNA in FFPE tissues or cells with DIGX® anti-digoxigenin linker, POLYVIEW® PLUS detection reagents combined with HIGHDEF® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW™ Hybridization Buffer (1X), included in this set.
