AMPIVIEW™ RNA probes are uniquely designed with the precision of targeted, sequence-specific RNA probes, powered by Enzo's LoopRNA ISH™ technology to deliver superior sensitivity. The AMPIVIEW™ HPV High-Risk RNA Probe is a mixture of digoxigenin-labeled probes targeting HPV type 16, HPV type 18, HPV 31, HPV 33 and HPV 51 for in situ hybridization. The probes are formulated in a buffered formamide solution with hybridization enhancers.
HPV infected cervical tissue tested positive for HPV 16/18/31/33/51 (red) detected with AMPIVIEW™ HPV High Risk RNA Probes (ENZ-GEN148)
Normal cervical tissue tested negative for HPV 16/18/31/33/51 as no signal is detected with AMPIVIEW™ HPV High Risk RNA Probes (ENZ-GEN148)
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Product Details
Applications:
ISH (in situ hybridization)
Application Notes:
AMPIVIEW™ HPV High-Risk RNA probes have been designed to target the human papillomavirus (HPV) type 16 and type 18 for its detection in tissues and cells. AMPIVIEW™ HPV High-Risk RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo's nanopolymer-based detection system, POLYVIEW® Plus, DIGX® anti-digoxigenin linker and , high-quality chromogens and counterstains, HIGHDEF® to produce clear results that can be visualized with a light microscope.
Aliquot and store at -20ºC or -80ºC. Under these conditions, products are stable until its expiration dates.
Handling:
Allow contents to warm up to room temperature prior to use.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Technical Info/Product Notes:
AMPIVIEW™ HPV High-Risk RNA Probes are optimized for detection with either POLYVIEW® PLUS detection reagents, HIGHDEF® chromogens and counterstains (not inlcuded). Dilutions and concentration optimization can be done with AMPIVIEW™ hybridization buffer, included in this set.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Recent improvements in immunohistochemistry and in situ hybridization: Gerard J. Nuovo, Jack Coleman, Maurizio Mauro; In Situ Molecular Pathology and Co-Expression Analyses 2nd Edition, 213-233 (2020), Book Chapter, Abstract;
Recent improvements in in situ hybridization for the detection of HPV infections in clinical samples: G. La Rocca, J. Coleman, E. Rabbani, G. Palermo, F. Palermo, M. Mauro; World Cancer Research Journal WCRJ 2020; 7: e1542, (2020), Abstract;
