Positive and negative control RNA probes labeled with digoxigenin for in situ hybridization (ISH) in cells and tissues.
ENZ-KIT224-0040
40 tests
500.00 USD
AMPIVIEW™ GAPDH (AS) Dig RNA Probes are digoxigenin-labeled RNA probes targeting GAPDH nucleic acid (DNA/RNA or RNA) for in situ hybridization in tissues. GAPDH has been used as a housekeeping gene due to its ubiquitous nature. AMPIVIEW™ NSP Dig RNA probes have been designed with a non-specific sequence to use as a negative control. The probes are formulated in a buffered formamide solution with hybridization enhancers.
GAPDH (red) detected in prostate tissue with AMPIVIEW™ GAPDH (AS) Dig RNA probes, DIGX® rabbit anti-digoxigenin linker, POLYVIEW® PLUS AP (anti-rabbit) Detection reagent combined with HIGHDEF® Red AP IHC chromogen and counterstained with HIGHDEF® Hematoxylin.
No signal detected in prostate tissue with AMPIVIEW™ NSP Dig RNA probes, DIGX® anti-digoxigenin linker, POLYVIEW® PLUS AP Detection reagent combined with HIGHDEF® Red AP IHC chromogen and counterstained with HIGHDEF® Hematoxylin.
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Product Details
Applications:
ISH (in situ hybridization)
Application Notes:
AMPIVIEW™ GAPDH and NSP Dig Control Probes Kit contains AMPIVIEW™ GAPDH (AS) Dig RNA probes (positive control) and NSP Dig RNA probes (negative control) for in situ hybridization in tissues and cells. AMPIVIEW™ GAPDH (AS) Dig RNA probes have been designed to target human glyceraldehyde-3- phosphate dehydrogenase (GAPDH) in tissues and cells. AMPIVIEW™ NSP Dig RNA probes have been designed with a non-specific sequence to use as a negative control. Both probes have a digoxigenin label and have been optimized to produce clear results with Enzo's DIGX® anti-digoxigenin linker and nanopolymner-based detection systems such as POLYVIEW® PLUS, combined with HIGHDEF® chromogens and counterstain to produce clear results that can be visualized with a light microscope.
Species reactivity:
Human
Contents:
2 x 1 mL AMPIVIEW™ GAPDH (AS) Dig RNA Probes (2µg/mL)
2 x 1 mL AMPIVIEW™ NSP Dig RNA Probes (RTU)
1 x 2 mL AMPIVIEW™ Hybridization Buffer (1X)
Use/Stability:
Aliquot and store at -20°C or -80°C. Under these conditions, products are stable until its expiration dates.
Handling:
Allow contents to warm up to room temperature prior to use.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Technical Info/Product Notes:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic and key regulatory enzyme and most commonly used as a housekeeping gene.
AMPIVIEW™ GAPDH (AS) Dig RNA Probes are optimized for detection of GAPDH RNA or RNA/DNA in FFPE tissues or cells. AMPIVIEW™ NSP Dig RNA Probes are ready-to-use probes that have been designed with a non-specific sequence. Both probes can be detected with DIGX® anti-digoxigenin linker, POLYVIEW® PLUS detection reagents combined with HIGHDEF® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW™ Hybridization Buffer (1X), included in this kit.