A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection GAPDH RNA or DNA/RNA in cells and tissues
ENZ-GEN127-2000
2 ml
300.00 USD
AMPIVIEW™ GAPDH (AS) Dig RNA Probes are digoxigenin-labeled RNA probes targeting ubiquitin nucleic acid (DNA/RNA or RNA) for in situ hybridization in tissues and cells. The probes are formulated in a buffered formamide solution with hybridization enhancers.
GAPDH (red) detected in prostate tissue with AMPIVIEW™ GAPDH (AS) Dig RNA probes, DIGX® rabbit anti-digoxigenin linker, POLYVIEW® PLUS AP (anti-rabbit) Detection reagent combined with HIGHDEF® Red AP IHC chromogen and counterstained with HIGHDEF® Hematoxylin.
No signal detected in prostate tissue with AMPIVIEW™ NSP Dig RNA probes, DIGX® anti-digoxigenin linker, POLYVIEW® PLUS AP Detection reagent combined with HIGHDEF® Red AP IHC chromogen and counterstained with HIGHDEF® Hematoxylin.
Please mouse over
Product Details
Applications:
ISH (in situ hybridization)
Application Notes:
AMPIVIEW™ GAPDH (AS) Dig RNA probes have been designed to target human glyceraldehyde-3- phosphate dehydrogenase (GAPDH) in tissues and cells. AMPIVIEW™ GAPDH (AS) Dig RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo's DIGX® anti-digoxigenin linker and nanopolymner-based detection systems, POLYVIEW® PLUS, combined with HIGHDEF® chromogens and counterstain to produce clear results that can be visualized with a light microscope.
Species reactivity:
Human
Contents:
2 x 1 mL AMPIVIEW™ GAPDH (AS) Dig RNA Probes (2µg/mL)
1 x 2 mL AMPIVIEW™ Hybridization Buffer (1X)
Use/Stability:
Aliquot and store at -20°C or -80°C. Under these conditions, products are stable until its expiration dates.
Handling:
Allow contents to warm up to room temperature prior to use.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Technical Info/Product Notes:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic and key regulatory enzyme and most commonly used as a housekeeping gene.
AMPIVIEW™ GAPDH (AS) Dig RNA Probes are optimized for detection of ubiquitin RNA or RNA/DNA in FFPE tissues or cells with DIGX® anti-digoxigenin linker, POLYVIEW® PLUS detection reagents combined with HIGHDEF® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW™ Hybridization Buffer (1X), included in this set.
