A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection ubiquitin RNA or DNA/RNA in cells and tissues.
ENZ-GEN125-2000
2 ml
300.00 USD
AMPIVIEW™ Ubiquitin (AS) Dig RNA Probes are digoxigenin-labeled RNA probes targeting ubiquitin nucleic acid (DNA/RNA or RNA) for in situ hybridization in tissues and cells. The probes are formulated in a buffered formamide solution with hybridization enhancers.
Ubiquitin (red) detected in prostate tissue with AMPIVIEW™ Ubiquitin (AS) Dig RNA probes, DIGX® anti-digoxigenin linker, POLYVIEW® PLUS AP Detection reagent combined with HIGHDEF® Red AP IHC chromogen and counterstained with HIGHDEF® Hematoxylin.
No signal detected in prostate tissue with AMPIVIEW™ NSP Dig RNA probes, DIGX® anti-digoxigenin linker, POLYVIEW® PLUS AP Detection reagent combined with HIGHDEF® Red AP IHC chromogen and counterstained with HIGHDEF® Hematoxylin.
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Product Details
Applications:
ISH (in situ hybridization)
Application Notes:
AMPIVIEW™ Ubiquitin (AS) Dig RNA probes have been designed to target ubiquitin in tissues and cells. AMPIVIEW™ Ubiquitin (AS) Dig RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo's DIGX® anti-digoxigenin linker and nanopolymner-based detection systems, POLYVIEW® PLUS, combined with HIGHDEF® chromogens and counterstain to produce clear results that can be visualized with a light microscope.
Species reactivity:
Human, Mouse, Rat
Contents:
2 x 1 mL AMPIVIEW™ Ubiquitin (AS) Dig RNA Probes (2µg/mL)
1 x 2 mL AMPIVIEW™ Hybridization Buffer (1X)
Use/Stability:
Aliquot and store at -20°C or -80°C. Under these conditions, products are stable until its expiration dates.
Handling:
Allow contents to warm up to room temperature prior to use.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Technical Info/Product Notes:
Ubiquitin is a regulatory protein found in nearly all eukaryotic organism. It is highly conserved and virtually identical across all forms of life and ideal to use as a positive control.
AMPIVIEW™ Ubiquitin (AS) Dig RNA Probes are optimized for detection of ubiquitin RNA or RNA/DNA in FFPE tissues or cells with DIGX® anti-digoxigenin linker, POLYVIEW® PLUS detection reagents combined with HIGHDEF® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW™ Hybridization Buffer (1X), included in this set.