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AMPIVIEW™ HPV 31/33/51 RNA Probes Set

A mixture of digoxigenin-labeled RNA probes for the detection of HPV 31/33/51 for in situ hybridization.
 
ENZ-GEN147-2000 2 ml Inquire for pricing
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AMPIVIEW™ RNA probes are uniquely designed with the precision of targeted, sequence-specific RNA probes powered by Enzo's LoopRNA ISH™ technology to deliver superior sensitivity. The AMPIVIEW™ HPV 31/33/51 RNA Probe is a mixture of digoxigenin-labeled probes targeting HPV type 31, HPV type 33 and HPV type 51 for in situ hybridization. The probes are formulated in a buffered formamide solution with hybridization enhancers.
ENZ-GEN147_HPV31-33-51_positive_cervical_tissue
HPV infected cervical tissue tested positive for HPV 31/33/51 (blue) detected with AMPIVIEW™ HPV 31/33/51 (ENZ-GEN147)
ENZ-GEN157_HPV31-33-51_negative_cervical_tissue
Normal cervical tissue tested negative for HPV 31/33/51 as no signal is detected with AMPIVIEW™ HPV 31/33/51 (ENZ-GEN147)
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ENZ-GEN147_HPV31-33-51_positive_cervical_tissue ENZ-GEN157_HPV31-33-51_negative_cervical_tissue

Product Details

Applications:ISH (in situ hybridization)
 
Application Notes:AMPIVIEW™ HPV 31/33/51 RNA probes have been designed to target the human papillomavirus (HPV) type 31, type 33 and type 51 for its detection in tissues and cells. AMPIVIEW™ HPV 31/33/51 RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo's nanopolymer-based detection system, POLYVIEW® Plus, DIGX® anti-digoxigenin linker and, high-quality chromogens and counterstains, HIGHDEF® to produce clear results that can be visualized with a light microscope.
 
Contents:AMPIVIEW™ HPV 31/33/51 RNA Probes
AMPIVIEW™ Hybridization Buffer (ENZ-ACC152)
 
Use/Stability:Stable for at least a year after receipt when stored as recommended. Use probe immediately after dilution with AMPIVIEW™ Hybridization Buffer (included).
 
Handling:Allow contents to warm up to room temperature prior to use.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Technical Info/Product Notes:AMPIVIEW™ HPV 31/33/51 RNA Probes are optimized for detection with either POLYVIEW® PLUS detection reagents, HIGHDEF® chromogens and counterstains (not inlcuded). Dilutions and concentration optimization can be done with AMPIVIEW™ hybridization buffer (ENZ-ACC152), included in this set.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Recent improvements in immunohistochemistry and in situ hybridization: Gerard J. Nuovo, Jack Coleman, Maurizio Mauro; In Situ Molecular Pathology and Co-Expression Analyses 2nd Edition, 213-233 (2020), Book Chapter, Abstract;
Recent improvements in in situ hybridization for the detection of HPV infections in clinical samples: G. La Rocca, J. Coleman, E. Rabbani, G. Palermo, F. Palermo, M. Mauro; World Cancer Research Journal WCRJ 2020; 7: e1542, (2020), Abstract;

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