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Better Detection Upstream.
Maximum Production Downstream.

Enabling Solutions for Bioprocess Optimization


  • Protein Aggregation
  • Thermal Shift Stability
  • PEGylated Proteins
  • Protein A

  • Small Molecules for Bioprocessing Applications
  • DiSH™ Hybridoma Screening & Cloning Kit

  • Simplify your cell death analysis
  • Sensitive assays for oxidative stress
  • Reliable detection of cell line instability

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Bioprocess Brochure

Enabling Solutions for Bioprocess Optimization

Development of a single drug, whether it is a new chemical entity, biotherapeutic, or genetic/cellular therapy, requires significant investment of resources. Each step of the process from early discovery through production and delivery must be fully explored, characterized, and understood to prevent issues resulting from cell stress, cell death, protein aggregation, and factors affecting reliable manufacturing of the drug. Whether you work in drug discovery, upstream, or downstream bioprocessing, Enzo offers a range of products to help you maintain cell line viability, optimize and monitor product integrity, and maximize yield.

Featured Tutorial



Optimizing Biologics Formulations : Quantifying Trace Subvisible Protein Aggregates Using a Protein Aggregation Assay and Standards.”
Wayne F. Patton, Ph.D.
Read the tutorial
 
Small Molecules ELISA and Detection Kits
Osmotic Stress Modulators Oxidative Stress
Hybridoma Modulators Cell Death: Autophagy, Apoptosis, Necrosis
Autophagy Inhibitors Cell Line Instability
Compound Libraries IgG ELISA Kits
Toxicity Protein A
Protease Inhibitors PEGylated Protein
Bioactives Thermal Shift (Stability)
Hybridoma Production Refolding
DiSH Kit Protein Aggregates

Optimize and Monitor Product Integrity In Your Process

ProteoStat® Protein Aggregation Assay

The ProteoStat® Protein Aggregation Assay is a simple, sensitive, homogeneous fluorescent microplate assay. The novel molecular rotor dye used detects a broad range of different protein aggregates from visible to subvisible particles.
  • Delivers superior results when compared to conventional protein detection dyes (Sypro Orange, Thioflavin T, ANS, Congo Red)
  • Works over a wide pH and ionic strength range and is compatible with commonly used buffers and excipients
  • Provides a convenient, complementary orthogonal method for cross-validation of instrumentation-intensive techniques
  • Useful assay to troubleshoot protein damage, from protein development through manufacturing stages



ProteoStat® Thermal Shift Stability Assay

The ProteoStat® Thermal Shift Stability Assay is a rapid, simple screening method based on a novel molecular rotor dye which detects protein aggregation. The assay does not require any prior knowledge of protein structure, sequence or ligand binding activity, and is performed on a thermally regulated fluorimeter or RT-PCR instrument. A related kit is available for the screening of optimal protein refolding parameters.


  • Perform accelerated screening for protein stability as a function of pH, ionic strength, and concentration
  • Screen for ligand binding to proteins of unknown function
  • Screen protein variants to determine their relative stabilities or excipients to determine optimal protein storage conditions
  • Identify protein-protein interaction inhibitors
  • ProteoStat® dye is tolerant of detergents and hydrophobic compounds

PEGylated Protein ELISA Kit

The sensitive PEGylated Protein ELISA kit is ideal for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and in-process QC studies.
  • Validated for a wide range of MW linear and branched PEGs, both in free form and when conjugated to proteins
  • Sensitive assay measures < 1ng/ml of PEGylated molecules
  • Quantifies PEGylated target molecules in complex matrices to allow monitoring of drug levels or its accumulation in tissue
  • High throughput format allows analysis of up to 35 samples in duplicate in just 2 hours



Sensitive detection of linear and branched PEG molecules

Protein A ELISA Kit

The Protein A ELISA kit is a sensitive and reproducible sandwich assay to quantify Protein A residuals in monoclonal antibody preparations. This extensively validated ELISA kit enables efficient detection of natural and recombinant Protein A constructs with up to 100% recovery.


  • Detects 1ppm of Protein A residuals in human IgG
  • Recognizes 4 different Protein A variants
  • Useful for contamination analysis and measurement of Protein A variants in monoclonal antibody preparations
  • Produces results in less than 3 hours with low cost per test

Maximize the Yield of Your Biotherapeutics

Small Molecules for Bioprocessing Applications

Cell culture and process optimization can benefit from the addition of several compounds which increase biotherapeutic yield or reduce the risk of cell line instability. Enzo offers an extensive collection of high-purity, cost-effective small molecules and proteins which can be used to optimize the production process both on a small and a large scale.
  • Ultra-high purity
  • Cost-effective
  • Extensively validated

Standalone Biochemicals

Autophagy Inhibitors, Osmotic Stress Inhibitors, Protease Inhibitors, and more

Compound Libraries




Reduce Hybridoma Screening & Cloning Time by 75%

DiSH™ Kit

The DiSH™ Kit is a unique technology for the rapid production of hybridomas. Using a genetically-modified myeloma partner, the kit creates hybridomas capable of both secreting and surface-expressing antigenspecific antibodies, enabling screening and selection of positive clones by FACS. The components of the kit are available as standalone products as well.
  • Enables faster, improved selection of specific, stable hybridomas
  • Minimizes manipulation of cloned cell lines relative to approaches requiring Limiting Dilution Subcloning (LDS)
  • Decreases time and labor costs

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Maintain Cell Line Viability

Environmental variations in cell culture conditions can lead to cell line instability, cellular stress and/or cell death. Monitoring these processes is crucial to ensure the efficiency and functionality of small-scale cultures or large-scale bioreactors. Enzo offers complete solutions for monitoring a variety of cell processes.

Simplify your Cell Death Analysis

Enzo provides a variety of tools to detect the onset of cell death pathways, including sensitive fluorescent probes for detection of apoptosis, necrosis, and autophagy. These assays can help detect subtle changes in the health of adherent or suspension cell cultures used for production of biotherapeutics, and assess toxicity during drug development.

Autophagy and Apoptosis Assays

Sensitive Assays for Oxidative Stress

Changes in mitochondrial function and glucose metabolism can adversely affect cell line viability, decreasing protein yield in cultured cells and long-term efficacy of cell based therapies. Enzo provides an industry-leading portfolio of kits and reagents for monitoring oxidative stress, including sensitive, high-throughput compatible assays for hypoxia, reactive oxygen species (ROS) and mitochondrial dysfunction.

High-throughput analysis of mitotoxicity

Reliable Detection of Cell Line Instability

Changes in gene expression due to endogenous or transgene copy number loss, or epigenetic suppression can affect cell line stability and yield. Our portfolio of products for detection of cell line instability include fluorescent labeling kits for high-resolution array CGH, RNA amplification and labeling, and DNA methylation.

Superior dye incorporation results in more accurate detection



Related Literature and Documents

Latest Literature

Product Flyers
Bioprocess - Innovative Tools for Biotherapeutics Development
Bioprocess - Innovative Tools for Biotherapeutics Development 09-Sep-13
Download as PDF
Product Flyers
ProteoStat™ Thermal Shift Stability Assay
ProteoStat⢠Thermal Shift Stability Assay 31-May-10
Download as PDF
Product Flyers
ProteoStat™ Protein Aggregation Assay
ProteoStat⢠Protein Aggregation Assay 31-May-10
Download as PDF
Product Flyers
Protein A EIA Kit - Sensitive & Reliable Detection
Protein A EIA Kit - Sensitive & Reliable Detection 08-Jun-10
Download as PDF

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