Enabling Enhanced Production, Efficient Purification, and Preservation of Product Integrity
In the field of biopharmaceutical production, we understand that achieving optimal results is paramount. That’s why we prioritize process optimization to unlock its full potential. Our approach revolves around three crucial aspects: enhanced production, efficient purification, and the preservation of product integrity. Optimizing bioprocess development is critical for overcoming potential challenges stemming from cell stress, cell death, protein aggregation, contamination, and other factors that might impede the reliable manufacturing of the drug. We aim to streamline and enhance your bioprocessing workflow by providing cutting-edge solutions and technologies, leading to more efficient and successful biotherapeutic development.
Maximizing yield in biopharmaceutical production is crucial for optimizing productivity and cost-effectiveness. It involves strategic process optimization, such as enhancing cell culture conditions, optimizing fermentation parameters, and improving downstream purification techniques.
100-fold lower endotoxin levels than comparable products
Reliable results through consistent lot-to-lot performance
Achieve rapid expansion of primary B cells with MEGACD40L® recombinant protein. Using MEGACD40L® in association with other stimulants can improve expansion of primary B cells by 36 fold, based on the work by Hung KL, et al
B cell lymphocyte activation by various CD40L constructs. PBMCs were treated for 48 hrs in media containing serially diluted CD40L, CD40L + 2 μg/mL Enhancer, or MEGACD40L® Protein.
Small Molecules and Peptides
Incorporating small molecules into bioprocessing workflows can enhance productivity at various stages of the production cycle. These molecules act as potent enhancers, catalysts, or modulators, driving improvements in yield, quality, and overall process performance.
Our chemistry and compound range includes over 3,000 stand-alone small molecules with known activity, including natural products, enzyme inhibitors, receptor ligands, drugs, lipids, and fatty acids. From metabolic engineering to downstream processing, these compounds offer unprecedented opportunities to enhance productivity, streamline workflows, and achieve greater efficiency in producing valuable biologics.
By monitoring aggregation, product stability can be assessed, allowing for the optimization of formulation and manufacturing processes to meet stringent regulatory requirements. This proactive approach ensures the final biopharmaceutical product maintains its integrity, efficacy, and safety.
PROTEOSTAT® Protein Aggregation Assay
Based on an innovative molecular rotor dye, the PROTEOSTAT® Protein Aggregation Assay is a simple, sensitive, and homogeneous fluorescent microplate assay allowing the detection in solution of a broad range of protein aggregates, from visible to subvisible particles.
Delivers superior results when compared to conventional protein detection dyes
High-throughput method for monitoring protein stability when used with flow cytometry or microplate reader
Provides a convenient, complementary orthogonal method for cross-validation of instrumentation-intensive techniques
Useful assay to troubleshoot protein damage, from protein development through manufacturing stages
Broadest Detection Range from Visible to Subvisible Particles
Methods to analyze protein aggregation
PROTEOSTAT® Thermal Shift Stability Assay
Based on an innovative molecular rotor dye, the PROTEOSTAT® Thermal Shift Stability Assay is a rapid, simple, and sensitive screening method allowing the end user to investigate proteins’ aggregation propensity by determining the aggregation temperature.
Perform accelerated screening for protein stability as a function of pH, ionic strength, and concentration
Screen for ligand binding to proteins of unknown function
Development and Specificity of the PROTEOSTAT® Dye
Using PROTEOSTAT® for a Rapid Detection and Characterization of Protein Aggregates by Flow Cytometry
Detection of Bacterial Aggregates by Flow Cytometry
Using PROTEOSTAT® Reagents to Predict Aggregation
Propensity and Monitor Aggregation of Antibody-Drug Conjugates (ADC)
Monitor Contamination
Downstream Bioprocess - Process Monitoring/Quality Control
Contamination in biopharmaceuticals can occur at different stages of production and includes sources such as microorganisms, adventitious agents, and chemical impurities. Effective monitoring and control play a critical role in ensuring the safety and integrity of biopharmaceutical products while meeting rigorous regulatory standards.
Protein A ELISA Kit
Protein A ELISA kit is a sensitive and reproducible sandwich immunoassay to quantify Protein A residuals in monoclonal antibody preparations.
Highly sensitive measurement, detecting as little as 9.01 pg/ml (<1 ppm) of Protein A residuals in purified humanized mAb preparations
Universally used as it recognizes 4 commonly used constructs of Protein A
High-throughput format with results in under 3 hours for up to 37 samples in duplicate
Recognition of all Commonly Used Protein A Constructs Ensures Accurate Results
Protein A construct cross-reactivity assessment
Host Cell Protein ELISA Kits
The Host Cell Protein ELISA kits and reagents are ideal for impurity analysis. Quantitatively measure host cell protein contamination in E.coli, CHO, and HEK293T derived products.
Sensitive measurement of host cell protein, detecting as little as 10 ng/ml
High protein coverage greater than 70% thereby, reducing the risk of missing potential contaminants
High-throughput format with results in as little as 3 hours
7 Simple Tools For Maximizing Your Bioprocess Pipeline
Are you in the bioprocessing field?
If so, let Enzo help you navigate the daunting quality control aspects of your operation by providing 7 simple tools to maximize your bioprocess pipeline. Whether you work in drug discovery, upstream, or downstream bioprocessing, Enzo offers a range of products to help you optimize product integrity, monitor contamination, and maximize yield.
Who should attend this webinar?
Anyone in the bioprocessing field who wants to know more about characterizing their biologic, protein aggregation, formulation optimization, storage and packaging of their biologic, and how to monitor and detect contamination.
Why should you attend this webinar?
To ensure that you are optimizing your bioprocess pipeline to facilitate rigorous and reproducible quality control in order to produce the highest quality product while consistently and reliably scaling your operation.
Improve Pharmacokinetic Analysis - PEGylated Protein ELISA Kit
The sensitive PEGylated Protein ELISA kit is ideal for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and in-process QC studies.
Validated for a wide range of MW linear and branched PEGs, both in free form and when conjugated to proteins
Sensitive assay measures < 1ng/ml of PEGylated molecules
Quantifies PEGylated target molecules in complex matrices to allow monitoring of drug levels or its accumulation in tissue
High throughput format allows analysis of up to 35 samples in duplicate in just 2 hours
Targeted SCREEN-WELL® libraries for cardiotoxicity, hepatotoxicity, hematopoietic toxicity, nephrotoxicity and myotoxicity contain compounds with defined and diverse organ-associated toxicity profiles. A variety of structurally and mechanistically different compound classes are included, as well as non-toxic controls. Compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates at 100 or 500 μL per well. The libraries are useful for predictive toxicology screening, including high-content protocols.
Better understand mechanisms of toxicity with your small molecule or candidate-drug of interest
Compare your compound with known references for investigative toxicity