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Featured products for Immunohistochemistry

Convenient IHC Tools

High Definition IHC chromogens

IHC Validated Antibodies

Complete Immunohistochemistry Solution

Browse our complete IHC range

Visualize More. Consume Less.

IHC picture

For decades, immunohistochemistry (IHC) has been an invaluable tool for the detection, localization, and quantification of antigens in preserved tissue for research and diagnostic purposes. Continuing our position as a leader in detection technologies, Enzo Life Sciences offers a complete set of tools to help improve the quality and efficiency of your IHC process from start to finish. Our high-sensitivity, low background reagents, combined with the market’s most extensive palette of unique chromogens provide the flexibility needed for simple or complex protocols. Our convenient kits, an extensive catalog of IHC validated antibodies, and high definition chromogens enable accurate, reproducible multiplex detection of two or more antigens in the same sample, increasing efficiency and preserving precious tissue samples.

Complete Kits & Flexible Reagents for Immunohistochemistry

Featured Literature


Customer Testimonials

" Our lab studies the cell cycle in breast cancer model system. We were trying to determine the change in phosphorylation of a protein (p27kip1) in needle biopsy tissue samples of patients suffering from breast cancer. But in pilot experiments we found that not only phosphorylation, but total protein levels of p27kip1 changed in these tissues, requiring us to double stain them. We found a multi staining IHC kit from Enzo Lifesciences and upon requesting to the customer service, received a sample. The staining worked really well right away and we could easily separate the two colors (yellow and red) while staining the same protein. It was easy to troubleshoot and optimize the conditions to suit our system and the technical support was very helpful to answer any questions we had regarding the kit. All in all, we found the multi staining kit to be very easy and effective for our project."

Priyank Patel
Dept. of Cell Biology
SUNY Downstate Medical Center

" Our research facility is involved in understanding key aspects of cancer cell behavior from a wide range of tumor models and tissue types. One of the main tools we utilize to investigate this is immunohistochemistry on formalin-fixed paraffin-embedded tissue thus allowing critical components to be identified that may allow for novel therapies to be developed. We tested Enzo’s POLYVIEW® IHC detection reagents and HIGHDEF® chromogens on our immunohistochemical autostainers against similar available detection reagents and chromogens. We found that the Enzo’s detection reagents and chromogens matched or exceeded what we currently used, allowing for increased sensitivity and specificity when used against both human and murine tissue. "

Colin Nixon
Beatson Institute for Cancer Research, Glasgow, UK

Read the Application Note developed in collaboration with his team: Compatibility of POLYVIEW® IHC Detection Reagents and HIGHDEF® IHC Chromogens with Automated IHC Slide Stainers


Convenient Tools for Complex Protocols


Superior MULTIVIEW® IHC kits for efficient detection of multiple antigens in a single tissue sample.


Highest sensitivity, lowest background POLYVIEW® nanopolymer detection reagent for use with HIGHDEF® chromogens.


High sensitivity, low background SAVIEW® IHC detection kits.

Tissue Microarray

Our unique MULTIVIEW® Universal Tissue Microarrays enable rapid IHC screening of 11 different tissue specimens on a single slide.

An Unrivaled Selection of High Definition Chromogens

Enzo offers the broadest (and expanding) palette of IHC stain colors to choose from, giving you the ultimate flexibility in planning your multiplex Immunohistochemistry protocol.

  • Widest panel of color choices
  • Unique yellow, black and blue HRP chromogen
  • Increased flexibility with HRP & AP options
  • High definition color development
IHC picture of formalin-fixed paraffin-embedded human tonsil

Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF® IHC chromogen substrate (DAB, HC) (Prod. no. ADI-950-211, brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF® blue IHC chromogen (AP) (Prod. no. ADI-950-150, blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) (Prod. no. ADI-950-141, red), high molecular weight cytokeratin labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, yellow), and methyl green nuclear counterstain.


Detect With Confidence: Worry-Free IHC Antibodies

Our growing list of over 1000 IHC validated antibodies includes those for the detection of key signaling proteins, cell surface markers, mediators of cell death, oxidative stress, heat shock proteins, proteasomes, and more. We know IHC optimization can be a time and resource consuming endeavor. You can back any of our antibodies with our Worry-Free Antibody Trial Program, even those not validated for IHC.

Search for IHC Validated Antibodies

Neuroscience Antibodies for IHC

Download our brochure featuring IHC validated antibodies for neuroscience.

Neuroscience Antibodies for IHC

Providing the Whole Assay: Retrieval to Coverslip

No need to go anywhere else for the ancillary reagents and materials you need for your IHC process. Enzo offers a broad selection of components to enhance your data and simplify your ordering process at the same time.

Antigen Retrieval Reagents Dilutents & Salts Chromogen Enhancers Chromogen Enhancers Mounting Medium

Related Literature and Documents

Latest Literature

Neuroscience Antibodies for IHC

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