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Immunohistochemistry

IHC

Featured products for Immunohistochemistry

Convenient IHC Tools

High Definition IHC chromogens

IHC Validated Antibodies

Complete Immunohistochemistry Solution

Browse our complete IHC range

Eliminate Data Ambiguity

IHC picture

High Sensitivity. Low Background. True Results.

For decades, immunohistochemistry (IHC) has been an invaluable tool for the detection, localization, and quantification of antigens in preserved tissue for research and diagnostic purposes. Enzo’s complete set of tools improve the quality and efficiency of your IHC process from start to finish. Avoid ambiguity with high-sensitivity and low background reagents that are compatible with manual and automated platforms. Combined with the market’s most extensive palette of unique chromogens, Enzo provides the flexibility needed for simple or complex protocols.

 

" The POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) kit worked very well; there was no background and the protocol is faster than many other IHC kits available commercially. I would surely recommend it. I was really happy with the POLYVIEW® PLUS HRP-DAB (Anti-Mouse) kit too. "

– Columbia University

" We tested Enzo’s POLYVIEW® IHC detection reagents and HIGHDEF® chromogens on our immunohistochemical autostainers against similar available detection reagents and chromogens. We found that Enzo’s detection reagents and chromogens matched or exceeded what we currently used, allowing for increased sensitivity and specificity when used against both human and murine tissue. "

– The Beatson Institute

 

Enzo's IHC Platform

Eliminates false positive results

Consistent, reliable, and sharp results

Compatible with both manual and automated platforms

Cost-effective and time-saving

Application Notes:

Compatibility of POLYVIEW® IHC Detection Reagents and HIGHDEF® IHC Chromogens with Automated IHC Slide Stainers
This application note demonstrates the use of Enzo Life Sciences’ POLYVIEW® IHC detection reagents and HIGHDEF® IHC chromogen with Dako Autostainer Link 48 and Biocare Medical intelliPATH™ FLX as open system platforms to stain key biomarkers used in both fundamental and clinical cancer research.

Characterization of Immune Cell Infiltrates in Human Cardiac Allograft Vasculopathy Using MULTIVIEW® IHC Kit
This application note demonstrates the use of the Enzo Life Sciences’ MULTIVIEW® IHC Kit to multiplex the detection of immune cells in cardiac allograft vasculopathy.

Using Mouse-on-Mouse IHC Kits in Various Sample Types
This application note demonstrates the superiority of Enzo Life Sciences’ POLYVIEW® (mouse on mouse-HRP) IHC kit for the detection of antigens in mouse tissue using primary antibodies

Complete Kits & Flexible Reagents for Immunohistochemistry

TISSUE MICROARRAY

  • Diverse set of 11 tissues
  • Reactivity for over 130
    biomarkers
  • Rapid, inexpensive
    screening tools

MULTIPLEX

  • Conserve precious
    samples
  • Reduce reagent costs
  • Convenient kit format

SINGLE COLOR

  • High sensitivity, low background
    detection
  • Vibrant, unique chromogens
  • Ancillary reagents

full range of IHC reagents
 

An Unrivaled Selection of High Definition Chromogens

Enzo offers the broadest (and expanding) palette of IHC stain colors to choose from, giving you the ultimate flexibility in planning your multiplex Immunohistochemistry protocol.

 
  • Widest panel of color choices
  • Unique yellow, black and blue HRP chromogen
  • Increased flexibility with HRP & AP options
  • High definition color development
IHC picture of formalin-fixed paraffin-embedded human tonsil

Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF® IHC chromogen substrate (DAB, HC) ( brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF® blue IHC chromogen (AP) (blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) ( red), high molecular weight cytokeratin labeled with HIGHDEF® yellow IHC chromogen (HRP) (yellow), and methyl green nuclear counterstain.

 

Convenient Tools for Complex Protocols

Multiplex
Detection

Superior MULTIVIEW® PLUS IHC kits for efficient detection of multiple antigens in a single tissue sample.

Nanopolymer
Detection

Highest sensitivity, lowest background POLYVIEW® PLUS nanopolymer detection reagent for use with HIGHDEF® chromogens.

Streptavidin
Detection

High sensitivity, low background SAVIEW® PLUS IHC detection kits.

Tissue Microarray
Slides

Our unique MULTIVIEW® Universal Tissue Microarrays enable rapid IHC screening of 11 different tissue specimens on a single slide.

Detect With Confidence: Worry-Free IHC Antibodies

Our growing list of over 1000 IHC validated antibodies includes those for the detection of key signaling proteins, cell surface markers, mediators of cell death, oxidative stress, heat shock proteins, proteasomes, and more. We know IHC optimization can be a time and resource consuming endeavor. You can back any of our antibodies with our Worry-Free Antibody Trial Program, even those not validated for IHC.

Search for IHC Validated Antibodies

Neuroscience Antibodies for IHC

Download our brochure featuring IHC validated antibodies for neuroscience.

Brochures
Neuroscience Antibodies for IHC
 

Providing the Whole Assay: Retrieval to Coverslip

No need to go anywhere else for the ancillary reagents and materials you need for your IHC process. Enzo offers a broad selection of components to enhance your data and simplify your ordering process at the same time.

 
Antigen Retrieval Reagents Dilutents & Salts Chromogen Enhancers Chromogen Enhancers Mounting Medium

Related Literature and Documents

Latest Literature

Brochures
Immunohistochemistry
Brochures
Neuroscience Antibodies for IHC
 
 

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