Product Details
| Alternative Name: | CK1 |
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| Clone: | 34βB4 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Keratin solubilized from human stratum corneum. |
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| UniProt ID: | P04264 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human
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| Specificity: | 34βB4 is specific for “high molecular weight” cytokeratin 1, 68 kDa, of “complex” epithelium. The antibody reacts with all squamous epithelium and squamous carcinomas. It does not react with adenocarcinomas of either simple epithelium (hepatoma) or ductal origin (breast, pancreas). |
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| Applications: | IHC (PS)
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections; for optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection). A 20- to 50-fold dilution of the antibody is suggested. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.1% sodium azide and 1% BSA. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human skin tissue using Cytokeratin 1 (human), mAb (34βB4) at a dilution of 1:20.
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Product Literature References
Allogeneic transplantation of epidermal cell sheets followed by endoscopic submucosal dissection to prevent severe esophageal stricture in a porcine model: S. Kobayashi, et al.; Regen. Ther.
21, 157 (2022),
Abstract;
Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues: A.M. Gown & A.M. Vogel; Am. J. Pathol.
114, 309 (1984),
Abstract;
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