| ENZ-ABS678-0100 | 100 µl | 149.00 USD |  |
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| ENZ-ABS678-1000 | 1 ml | 1,287.00 USD | |
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| Alternative Name: | MKI67 |
| Host: | Mouse |
| Isotype: | IgG |
| Immunogen: | Recombinant Ki-67. |
| UniProt ID: | P46013 |
| Species reactivity: | Human |
| Applications: | IHC |
| Recommended Dilutions/Conditions: | Immunohistochemistry (1:100-1:400) Optimal conditions must be determined individually for each application. |
| Application Notes: | For IHC applications: Dilute the concentrate as appropriate in Antibody Blocking Buffer (ENZ-ACC108), and then apply 150 µL to the slide, as indicated in the user's IHC protocol. |
| Purity Detail: | Affinity-purified. |
| Formulation: | Liquid. In Tris buffer, pH 7.5, containing 1% BSA and <0.1% sodium azide. |
| Use/Stability: | When stored correctly, the antibody is stable until the date indicated on the label. To ensure proper stability of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Blue Ice |
| Long Term Storage: | +4°C |
| Scientific Background: | Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumors, including those of the brain, breast, cervix, and prostate. |
| Protocol: | Manual Use
Automated Staining For automated staining, we recommend incubating for 30 minutes with the primary antibody and using Antigen Retrieval at pH 9 for 30 minutes. |
| Regulatory Status: | RUO - Research Use Only |
p16INK4a monoclonal antibody |
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| Monoclonal antibody to p16 for use with IHC applications | ||||||
| IHC, WB | Print as PDF | ||||||
| ENZ-ABS377-0100 | 100 µl | 185.00 USD | |
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| ENZ-ABS377-1000 | 1 ml | 1,678.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
POLYVIEW® PLUS HRP (anti-mouse) reagent |
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| High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC104-0150 | 150 tests | 183.00 USD | |
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POLYVIEW® PLUS AP (anti-mouse) reagent |
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| High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC114-0150 | 150 tests | 183.00 USD | |
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Antibody Blocker/Diluent |
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| Ready-to-use antibody blocking buffer and diluent, for use with POLYVIEW® PLUS detection reagents | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC108-0100 | 100 ml | 257.00 USD | |
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HIGHDEF® DAB Chromogen/Substrate Set |
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| Brown chromogen for use with HRP immunohistochemistry staining systems | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC105-0200 | 200 ml | 267.00 USD | |
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IHC/ISH Peroxidase Block |
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| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC107-0100 | 100 ml | 448.00 USD | |
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HIGHDEF® Hematoxylin |
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| Ready-to-use histologic stain with enhancers | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC106-0100 | 100 ml | 61.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
