Immunohistochemistry (1:50-1:200)
Optimal conditions must be determined individually for each application.
Application Notes:
The CA-125 antibody can be used to qualitatively identify the presence of CA-125 protein in FFPE tissue sections using IHC test methods.
For IHC applications: Dilute the concentrate as appropriate in antibody diluent, and then apply 150 µL to the slide, as indicated in the user's IHC protocol.
Purity Detail:
Affinity-purified.
Formulation:
Liquid. In Tris buffer, pH 7.5, containing 1% BSA and <0.1% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Scientific Background:
CA-125 is a key marker for ovarian carcinomas. It reacts with a majority of epithelial ovarian neoplasms of various types including serous, endometrioid, clear cell and undifferentiated but is also found in other neoplasms.
Regulatory Status:
RUO - Research Use Only
Formalin-fixed paraffin-embedded endometrial tissue stained using Enzo’s CA-125 antibody (Prod. No. ENZ-ABS775). IHC staining was performed on an automated Leica BOND III.
