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Oxidative Stress

Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage

Origins and Consequences of Oxidative Stress

The term oxidative stress reflects an imbalance in free radical formation within a cell or organism, most commonly in the form of reactive oxygen or nitrogen species (ROS/RNS). ROS/RNS such as superoxide anions, hydroxyl radicals, hydrogen peroxide, nitric oxide, and peroxynitrite originate from a variety of sources including changes in aerobic metabolism, immune activation, UV radiation, heme accumulation, and hypoxia. Failure of the cell’s defense mechanisms to compensate for accumulating insults such as mitochondrial dysfunction, DNA damage, misfolded proteins, and lipid peroxidation can trigger programmed cell death pathways, and has been linked to clinically relevant diseases including cancer, cardiovascular disease, asthma, ischemia, diabetes, and neurodegenerative disease.


Live Cell, Multiplex Analysis Kits

Accurately Profile Total ROS and Superoxide with Dual-readout Assay

ROS-ID® Total ROS/Superoxide Detection Kit

  • Non-invasive fluorescent probes allow simultaneous discrimination of total ROS and superoxide in live cells
  • Profile to distinguish between hydrogen peroxide, peroxynitrite and hydroxyl radicals using included inhibitors
  • Dyes compatible with major components of tissue culture media (phenol red, FBS and BSA) for microscopy and flow cytometry
  • Red dye for detection of Nitric Oxide also available (ENZ-51013 / ENZ-51001)

Multiplex Analysis of Total ROS AND Superoxide

Multiplex, Real-time Analysis of Hypoxia and ROS in Live Cells

ROS-ID® Hypoxia/Oxidative Stress Detection Kit

  • Sensitive hypoxia dye fluoresces red when converted by nitroreductases
  • Total ROS dye enables simultaneous detection of hypoxia (red) and ROS (green)
  • Non-toxic, cell permeable dyes optimized to avoid artifactual fluorescence
  • Suitable for microscopic or flow cytometric analysis of adherent or suspension cells

Simultaneously Monitor Hypoxic Status and ROS Formation

High-Throughput Assays for Mitochondrial Function

MITO-ID® Extracellular O2 Sensor Kits

This oxygen-sensitive phosphorescent probe can be used to assess O2 consumption by cultured cells, isolated mitochondria, microorganisms, tissues, and enzymes.

Detect Mitochondrial Dysfunction Within Minutes of Treatment

MITO-ID® Membrane Potential Cytotoxicity Assay Kit

A real-time mitochondrial membrane potential assay with superior sensitivity

Detect MMP Perturbations with 10X More Sensitivity than JC-1

Detect & Quantify Biomarkers of Oxidative Stress

As a trusted manufacturer of thousands of widely cited and thoroughly validated ELISA kits and antibodies, we understand quality means delivering sensitivity, specificity, and consistency. Over 300 immunoassays and 3000 antibodies enable sensitive & specific detection of protein and small molecule biomarkers of oxidative stress. Our Stressgen® assay kits and reagents are some of the most widely cited products for quantification of critical stress-regulating metabolic enzymes and stress-responsive pathways.

Product Highlight

DNA Damage (8-OHdG) ELISA Kit

  • Quantify levels < 1ng/mL
  • Rapid results in < 2.5 hours
  • Tested in a variety of biofluids (urine, serum, saliva)
  • Convenient colorimetric 96-well plate formats

Rapidly Detect <1 ng/ml 8-OHdG

Modulate with Pro-and Anti-Oxidant Compounds

REDOX Compound Library

The SSCREEN-WELL® REDOX Library contains 84 ready-to-screen compounds with defined prooxidant or antioxidant activity. All compounds come pre-dissolved in DMSO and are available in bulk quantities for resupply.

  • Hydroperoxides
  • Polyphenolics
  • Glutathione peroxidase inhibitors
  • Metal chelators
  • Thiols
  • Thiol traps
  • Lazaroids
  • Radical scavengers
  • SOD mimetics

Spin Traps & Spin Probes

High Purity & Stable Spin Traps

Spin trapping is a common analytical method for the detection of free radicals. The spin trap molecule (e.g., PBN, DMPO, TEMPO) reacts with a typically transient free radical species, thus “trapping” the free radical in the form of a more stable spin adduct. Adducts can then be detected using electron paramagnetic resonance (EPR) spectroscopy.

Enzo offers a variety of spin traps and probes for the detection of general or specific free radical species. Our compunds are of the highest purity, ensuring the most consistent results.

  • Highest purity spin traps for in vitro and in vivo applications
  • No additional purification is required
  • All products are quality tested by ESR spectroscopy

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