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Live Cell, Multiplex Analysis Kits

Accurately Profile Total ROS and Superoxide with Dual-readout Assay
Total ROS/Superoxide Detection Kit

  • Non-invasive fluorescent probes allow simultaneous discrimination of total ROS and superoxide in live cells
  • Profile to distinguish between hydrogen peroxide, peroxynitrite and hydroxyl radicals using included inhibitors
  • Dyes compatible with major components of tissue culture media (phenol red, FBS and BSA) for microscopy and flow cytometry
  • Red dye for detection of Nitric Oxide also available (ENZ-51013 / ENZ-51001)

Multiplex Analysis of Total ROS AND Superoxide

Data represents treatment of HeLa cells with Pyocyanin (ROS/SO inducer). Total ROS dye fluoresces green in the presence of ROS, while the SO-specific dye fluoresces yellow/orange (bottom right).

Convenient Quantifiable Flow Cytometry Assay

Data represents % of positive HeLa cells following treatment with Pyocyanin (ROS/SO inducer), TBHP (ROS Inducer), or AMA (Superoxide inducer).

 

ROS-ID® Total ROS/Superoxide detection kit 

Widely cited kit to detect total ROS and Superoxide in live cells by microscopy and flow cytometry applications
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51010 1 Kit 320.00 USD
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ROS-ID® Total ROS detection kit 

Widely cited kit to measure global levels of ROS in live cells
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51011 1 Kit 214.00 USD
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ROS-ID® Superoxide detection kit 

Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51012 1 Kit 218.00 USD
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Multiplex, Real-time Analysis of Hypoxia and ROS in Live Cells
NEW CYTO-ID® Hypoxia/Oxidative Stress Detection Kit

  • Sensitive hypoxia dye fluoresces red when converted by nitroreductases
  • Total ROS dye enables simultaneous detection of hypoxia (red) and ROS (green)
  • Non-toxic, cell permeable dyes optimized to avoid artifactual fluorescence
  • Suitable for microscopic or flow cytometric analysis of adherent or suspension cells

Simultaneously Monitor Hypoxic Status and ROS Formation

Quantitative Multiparametric Assay for Flow Cytometry

 

Bright red fluorescence of the Hypoxia probe is observed following its conversion by cellular nitroreductases under hypoxic conditions such as those induced chemically by treatment with the hypoxia-mimetic desferrioxamine (DFO). The assay facilitates simultaneous quantification of hypoxic status (FL3/red) and presence of ROS (FL1/green) by flow cytometry.

ROS-ID® Hypoxia/Oxidative stress detection kit 

Widely cited kit for the simultaneous analysis of hypoxia and oxidative stress by microscopy and flow cytometry applications
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51042-0125 125 tests 133.00 USD
 
ENZ-51042-K500 500 tests 463.00 USD
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