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High-Throughput Assays for Mitochondrial Function

MITO-ID® Extracellular O2 Sensor Kits

This oxygen-sensitive phosphorescent probe can be used to assess O2 consumption by cultured cells, isolated mitochondria, microorganisms, tissues, and enzymes.

  • Phosphorescent probe increases in signal intensity with O2 consumption (▼ O2 levels)
  • Amenable to 96- and 384-well microplates for high-throughput analysis on standard fluorescence plate readers (Ex/Em 380/650nm)
  • Cell permeable probe also available for tracing intracellular O2 levels
  • Multiplex with MITO-ID® pH Sensor probe to confirm mitochondrial toxicity

Detect Mitochondrial Dysfunction Within Minutes of Treatment


Assessment of mitochondrial function with MITO-ID® Extracellular O2 Sensor Kit (A) or traditional ATP assay (B) following treatment with mitochondrial inhibitors (Oligomycin, Rotenone, Antimycin), uncoupling agent (FCCP), or control (DMSO). Results illustrate that drug-induced mitochondrial dysfunction is evident immediately post-treatment (A) despite varying levels of viability @ 24 hours by ATP assay (B).

MITO-ID® Extracellular pH Sensor Probe

This pH-sensitive phosphorescent probe can be used to monitor cellular acid extrusion, the result of increased glycolytic flux associated with mitotoxicity.

  • pH sensitive probe increases in signal intensity with increasing acidity
  • Simple mix-and-read protocol for 96-well microplates
  • Amenable to standard fluorescence plate readers (Ex/Em 340/615nm)

Efficiently Monitor Glycolytic Activity

Acidification profiles of HepG2 cells treated with glucose transport inhibitors (2DG, Oxamate) or mitochondrial inhibitor (Antimycin) relative to control.


MITO-ID® Membrane Potential Cytotoxicity Assay Kit

A real-time mitochondrial membrane potential assay with superior sensitivity

  • 10X more sensitive than JC-1 with superior aqueous solubility
  • Photostable dual-emission dye
  • No-wash/no-medium removal
  • Separate MITO-ID® Red/Green assays available for detection of mitochondrial mass
  • Suitable for high-throughput applications

Detect MMP Perturbations with 10X More Sensitivity than JC-1

Dual-emission Dye Monitors Energetic Status


MITO-ID® Membrane potential cytotoxicity kit  

A Real-time Mitochondrial membrane Potential Assay with Superior Sensitivity
HTS, Microplate | Print as PDF
ENZ-51019-KP002 1 Kit 321.00 USD
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MITO-ID® Membrane potential detection kit  

The only assay that monitors energetic status using a simple mix-and-read, no-wash protocol
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
ENZ-51018-0025 25 tests 204.00 USD
ENZ-51018-K100 100 tests 565.00 USD
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