Tags: Cancer, Screening, Toxicology
Multidrug resistance (MDR) is a major impediment to the treatment of breast cancer. Disseminated cancers are often inherently resistant to a broad spectrum of anti-cancer drugs or, after an initial response phase, become resistant to further treatment. The multidrug resistance 1 gene (MDR1) encodes the plasma membrane efflux pump, P-glycoprotein (P-gp), and is a direct target of the Wnt/β-catenin signaling pathway. Overexpression of P-gp increases active efflux of cytotoxic drugs from cells, lowering intracellular concentrations and promoting chemoresistance. Dr. Gengyin Zhou and colleagues at the Shandong University School of Medicine in P. R. China (Zhang, et al Cancer Lett. 2012 Apr 4. [Epub ahead of print]) established that the Frizzled 1 (FZD1) protein, an essential component of the Wnt/β-catenin pathway, is overexpressed in the multidrug resistant breast cancer cell sub-line MCF-7/ADM, in concert with P-gp. FZD1 silencing by plasmid-mediated expression of small interference RNA (siRNA) diminished P-gp levels, restoring sensitivity to four tested chemotherapy drugs, while also significantly decreasing cytoplasmic and nuclear β-catenin levels. FZD1 offers promise as a potential new target for reversing multidrug resistance in breast cancer.
Enzo Life Sciences offers comprehensive tools contributing to the understanding of the canonical Wnt/β –catenin pathway, as well as the analysis of multi-drug resistance transporters.
Receive our TechNotes as soon as they are published.
LEADING LIGHT® Wnt Reporter Assay Starter Kit |
||||||
| Chemiluminescent cell-based assay for discovering Wnt pathway modulators | ||||||
| FUNC, Chemiluminescence, HTS | Print as PDF | ||||||
| ENZ-61001-0001 | 96 wells | 1,248.00 USD |  |
|||
| Do you need bulk/larger quantities? | ||||||
SCREEN-WELL® Wnt Pathway library |
||||||
| Compounds are highly relevant; many are unique | ||||||
| HTS | Print as PDF | ||||||
| BML-2838-0100 | 1 Library | 100 µl/well | 1,882.00 USD | |
||
| BML-2838-0500 | 1 Library | 500 µl/well | 4,507.00 USD | |
||
| Do you need bulk/larger quantities? | ||||||
EFLUXX-ID® Green multidrug resistance assay kit |
||||||
| Simple No-wash Assay for Simultaneous Monitoring of All 3 Major ABC Transporter Proteins – MDR, BCRP & MRP | ||||||
| Flow Cytometry, Fluorescence microscopy | Print as PDF | ||||||
| ENZ-51029-K100 | 1 Kit | 706.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
EFLUXX-ID® Gold multidrug resistance assay kit |
||||||
| Simple no-wash assays for simultaneous monitoring of all 3 major ABC transporter proteins – MDR, BCRP & MRP | ||||||
| Flow Cytometry, Fluorescence microscopy | Print as PDF | ||||||
| ENZ-51030-K100 | 1 Kit | 691.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Rhodamine 123 (ultra pure) |
||||||
| Mitochondria dye | ||||||
| 62669-70-9, ≥95% (HPLC) | Print as PDF | ||||||
| ENZ-52307 | 25 mg | 124.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Calcein AM (ultra pure) |
||||||
| Calcium dye | ||||||
| 148504-34-1, ≥95% (HPLC) | Print as PDF | ||||||
| ENZ-52002 | 1 mg | 176.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Hoechst 33342 (ultra pure) |
||||||
| Cell permeable DNA dye | ||||||
| 23491-52-3, ≥95% (HPLC) | Print as PDF | ||||||
| ENZ-52401 | 100 mg | 167.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
P-glycoprotein (human) monoclonal antibody (C494) |
||||||
| Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB | Print as PDF | ||||||
| ALX-801-003-C100 | 1 ml | 1,332.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
P-glycoprotein (human) monoclonal antibody (MRK16) |
||||||
| Flow Cytometry, IHC (FS), IP, FUNC | Print as PDF | ||||||
| ALX-801-008-C150 | 150 µg | 1,273.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
P-glycoprotein monoclonal antibody (C219) |
||||||
| ICC, IHC (FS), IHC (PS), IP | Print as PDF | ||||||
| ALX-801-002-C100 | 1 ml | 993.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Doxorubicin . HCl |
||||||
| Topoisomerase inhibitor | ||||||
| 25316-40-9, ≥95% (HPLC) | Print as PDF | ||||||
| BML-GR319-0005 | 5 mg | 109.00 USD | |
|||
| BML-GR319-0025 | 25 mg | 439.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Paclitaxel |
||||||
| Ultra-pure; stabilizes microtubules | ||||||
| 33069-62-4, Isolated from the bark of the Pacific Yew tree (Taxus brevifolia)., ≥98% (HPLC) | Print as PDF | ||||||
| BML-T104-0005 | 5 mg | 87.00 USD | |
|||
| BML-T104-0025 | 25 mg | 296.00 USD | |
|||
| BML-T104-0250 | 250 mg | 1,296.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Cisplatin |
||||||
| Antitumor agent | ||||||
| 15663-27-1, ≥98% (HPLC) | Print as PDF | ||||||
| ALX-400-040-M050 | 50 mg | 59.00 USD | |
|||
| ALX-400-040-M250 | 250 mg | 156.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
5-Fluorouracil |
||||||
| Thymidylate synthetase inhibitor | ||||||
| 51-21-8, ≥98% (HPLC) | Print as PDF | ||||||
| ALX-480-099-G005 | 5 g | 93.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
PGP-4008 |
||||||
| P-glycoprotein inhibitor | ||||||
| 365565-02-2, ≥93% | Print as PDF | ||||||
| ALX-270-290-M002 | 2 mg | 151.00 USD | |
|||
| ALX-270-290-M010 | 10 mg | 491.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Reversin 121 |
||||||
| P-glycoprotein inhibitor | ||||||
| ≥98% (HPLC) | Print as PDF | ||||||
| ALX-270-305-M005 | 5 mg | 344.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
