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HIGHDEF® Hematoxylin

Ready-to-use histologic stain with enhancers
 
ENZ-ACC106-0100 100 ml Inquire for pricing
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Available from stock, Ready for shipment.
HIGHDEF® Hematoxylin is intended for use in the histologic demonstration of nuclear staining. This staining technique is sometimes used to make the critical distinction between a normal nucleus and an abnormal one.

Product Specification

Formulation:Enhancers added to improve signal.
 
Applications:IHC
ISH (in situ hybridization)
 
Shipping:Ambient
 
Long Term Storage:Ambient
 
Technical Info/Product Notes:Hematoxylin is one of the most common staining compounds used in histology.

The protocols for specific applications can vary. These include, but are not limited to: fixation, heat-retrieval methods, incubation times, tissue section thickness, and detection kit used. Due to the superior sensitivity of these unique reagents, the recommended incubation times and titers listed are not applicable to other detection systems, as results may vary. Ultimately, it is the responsibility of the investigator to determine optimal conditions. These products are tools that can be used for interpretation of morphological findings in conjunction with other diagnostic tests and pertinent clinical data by a qualified pathologist.
 
Protocol:
  1. Deparaffinize and rehydrate tissue.
  2. Apply enough HIGHDEF® Hematoxylin to completely cover the tissue (usually about 150 μL) and incubate for 2-10 minutes, according to desired intensity.
  3. Rinse in deionized water for 20 to 30 seconds.
  4. Follow suggested dehydration and mounting procedures based on the chromogen used.
 
ENZ-ACC106 Hematoxylin-compare
Enzo's hematoxylin allows for clearer staining and less background than a leading competitor. Both slides were stained with Enzo's POLYVIEW® PLUS HRP-DAB Kit, but the competitor's hematoxylin was used on the slide on the right, showing more background and a less clear result.
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ENZ-ACC106 Hematoxylin-compare

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