Highest sensitivity, complete staining kit for both IHC and ISH
POLYVIEW® PLUS reagents' biotin free nanopolymer detection circumvents endogenous background while providing specific antigen localization
HIGHDEF® DAB (Brown) HRP provides high intensity color development to deliver sharp, crisp staining
Compatible with both manual and automated platforms
The POLYVIEW® PLUS HRP-DAB (Anti-Mouse) Kit is intended for both IHC and ISH staining. The kit is a non-biotin one step detection system suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and frozen sections. This kit can be used with blood smears, cytosmears, and cell preparations.
Cervical tissue Ki-67 detected using POLYVIEW® PLUS HRP-DAB (Anti-Mouse) Kit
Cervical tissue p16 detected using POLYVIEW® PLUS HRP-DAB (Anti-Mouse) Kit
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Product Details
Assay Time:
< 2 hours
Applications:
IHC ISH (in situ hybridization)
Handling:
Do not freeze.
Shipping:
Blue Ice Not Frozen
Long Term Storage:
+4°C
Contents:
IHC/ISH Peroxidase Block, Antibody Blocking Buffer, POLYVIEW® PLUS HRP (Anti-Mouse) Reagent (Ready-To-Use), HIGHDEF® DAB Chromogen, HIGHDEF® DAB Substrate Buffer, HIGHDEF® Hematoxylin, Antigen Retrieval Reagent, pH 9 (10X)
Technical Info/Product Notes:
The POLYVIEW® PLUS HRP-DAB (Anti-Mouse) Kit contains POLYVIEW® PLUS HRP (Anti-Mouse) Reagent that has been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyper-labeling technology in ready-to-use format. This ensures consistent and reproducible immunodetection of mouse antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The single step POLYVIEW® PLUS HRP (Anti-Mouse) Reagent allows faster staining procedures as compared to traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background. Enzo’s HIGHDEF® DAB (Brown) HRP and HIGHDEF® Hematoxylin reagents provide strong signal coupled with the peroxidase and antibody blocking blockers included in the kit to give minimal background.
