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POLYVIEW® PLUS HRP (anti-rabbit) reagent

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
 
ENZ-ACC103-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 
Available from stock, Ready for shipment.
  • Ready-to-use reagent
  • Biotin-free nanopolymer detection circumvents endogenous biotin background
  • High intensity color development delivers sharp, crisp staining
  • Compatible with both automated and manual platforms
  • The Antibody Blocker/Diluent further reduces background (sold separately)
  • Customize with HIGHDEF® chromogens of your choice (sold separately)
The POLYVIEW® PLUS HRP (anti-rabbit) reagent is a ready-to-use, biotin-free, one-step detection reagent suitable for immunohistochemical detection of antigens in formalin-fixed paraffin-embedded tissues and frozen sections. The detection reagent may also be used with blood smears, cytosmears, and cell preparations. In addition, the POLYVIEW® PLUS HRP (anti-rabbit) reagent can be adapted for in situ hybridization detection.

Product Specification

Applications:IHC
ISH (in situ hybridization)
 
Quantity:150 tests × 150 µL (22.5 mL total volume)
 
Handling:Do not freeze.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:The POLYVIEW® PLUS HRP (anti-rabbit) reagent has been developed by directly labeling anti-rabbit immunoglobulins with a nanopolymer of horseradish peroxidase (HRP) using proprietary technology. This ensures consistent and reproducible immunodetection of rabbit primary antibodies against nuclear, cytoplasmic, and membrane antigens in different types of tissues. The single step methodology enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background.

The POLYVIEW® PLUS HRP (anti-rabbit) reagent is suitable for use with rabbit IgG antibodies, both monoclonal and polyclonal. The reagent can be used for manual staining or with automated staining instruments and are well suited for multiplex immunohistochemical staining assays.

The Antibody Blocker/Diluent (ENZ-ACC108) further reduces background and is suitable for diluting the POLYVIEW® PLUS HRP (anti-rabbit) reagent.
 
ENZ-ACC103 IHC-Ki-67-POLYVIEW-PLUS-HRP-anti-rabbit
Cervical tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS HRP (anti-rabbit)
ENZ-ACC103 IHC-Ki-67-POLYVIEW-PLUS-HRP-anti-rabbit 2
Cervical tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS HRP (anti-rabbit)
ENZ-ACC103 IHC-Ki-67-POLYVIEW-PLUS-HRP-anti-rabbit 3
Cervical tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS HRP (anti-rabbit)
ENZ-ACC103 ISH-HRS-POLYVIEW-PLUS-HRP-anti-rabbit
Cervical tissue screened with PATHO-GENE® HPV Type 16/18/31/33/51 (ENZ-32882), then rabbit-anti-biotin linker (ENZ-32892), followed by POLYVIEW® PLUS HRP (anti-rabbit)
ENZ-ACC103 ISH-HRS-POLYVIEW-PLUS-HRP-anti-rabbit 2
Cervical tissue screened with PATHO-GENE® HPV Type 16/18/31/33/51 (ENZ-32882), then rabbit-anti-biotin linker (ENZ-32892), followed by POLYVIEW® PLUS HRP (anti-rabbit)
ENZ-ACC103 Ki67-POLYVIEW-PLUS-HRP- anti-rabbit
Tonsil tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS HRP (anti-rabbit) reagent
Please mouse over
ENZ-ACC103 IHC-Ki-67-POLYVIEW-PLUS-HRP-anti-rabbit ENZ-ACC103 IHC-Ki-67-POLYVIEW-PLUS-HRP-anti-rabbit 2 ENZ-ACC103 IHC-Ki-67-POLYVIEW-PLUS-HRP-anti-rabbit 3 ENZ-ACC103 ISH-HRS-POLYVIEW-PLUS-HRP-anti-rabbit ENZ-ACC103 ISH-HRS-POLYVIEW-PLUS-HRP-anti-rabbit 2 ENZ-ACC103 Ki67-POLYVIEW-PLUS-HRP- anti-rabbit

Product Literature References

Diagnostic pathology of Alzheimer's disease from routine microscopy to immunohistochemistry and experimental correlations: G. Nuovo, et al.; Ann. Diagn. Pathol. 28, 24 (2017), Abstract;
False-positive results in diagnostic immunohistochemistry are related to horseradish peroxidase conjugates in commercially available assays: G. Nuovo; Ann. Diagn. Pathol. 25, 54 (2016), Application(s): Compare secondary HRP conjugate from Leica, Ventana, and Enzo in side-by-side comparison on the Leica BOND-MAX, Abstract;

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