High intensity color development delivers sharp, crisp staining
Compatible with both automated and manual platforms
The Antibody Blocker/Diluent further reduces background (sold separately)
Customize with HIGHDEF® chromogens of your choice (sold separately)
The POLYVIEW® PLUS HRP (anti-rabbit) reagent is a ready-to-use, biotin-free, one-step detection reagent suitable for immunohistochemical detection of antigens in formalin-fixed paraffin-embedded tissues and frozen sections. The detection reagent may also be used with blood smears, cytosmears, and cell preparations. In addition, the POLYVIEW® PLUS HRP (anti-rabbit) reagent can be adapted for in situ hybridization detection.
The POLYVIEW® PLUS HRP (anti-rabbit) reagent has been developed by directly labeling anti-rabbit immunoglobulins with a nanopolymer of horseradish peroxidase (HRP) using proprietary technology. This ensures consistent and reproducible immunodetection of rabbit primary antibodies against nuclear, cytoplasmic, and membrane antigens in different types of tissues. The single step methodology enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background.
The POLYVIEW® PLUS HRP (anti-rabbit) reagent is suitable for use with rabbit IgG antibodies, both monoclonal and polyclonal. The reagent can be used for manual staining or with automated staining instruments and are well suited for multiplex immunohistochemical staining assays.
The Antibody Blocker/Diluent (ENZ-ACC108) further reduces background and is suitable for diluting the POLYVIEW® PLUS HRP (anti-rabbit) reagent.
Regulatory Status:
RUO - Research Use Only
Tonsil tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS HRP (anti-rabbit) reagent
Cervical tissue screened with PATHO-GENE® HPV Type 16/18/31/33/51 (ENZ-32882), then rabbit-anti-biotin linker (ENZ-32892), followed by POLYVIEW® PLUS HRP (anti-rabbit)
Cervical tissue screened with PATHO-GENE® HPV Type 16/18/31/33/51 (ENZ-32882), then rabbit-anti-biotin linker (ENZ-32892), followed by POLYVIEW® PLUS HRP (anti-rabbit)
Cervical tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS HRP (anti-rabbit)
Cervical tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS HRP (anti-rabbit)
Cervical tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS HRP (anti-rabbit)
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Product Literature References
Aerial part of Houttuynia cordata reverses memory impairment by regulating amyloid beta accumulation and neuroinflammation in Alzheimer's disease model: I.G. Ju, et al.; Phytother. Res. 37, 2854 (2023), Abstract;
Yomogin, Isolated from Artemisia iwayomogi, Inhibits Neuroinflammation Stimulated by Lipopolysaccharide via Regulating MAPK Pathway: J.H. Ki, et al.; Antioxidants 12, 106 (2023), Abstract;
H-Ferritin Produced by Myeloid Cells Is Released to the Circulation and Plays a Major Role in Liver Iron Distribution during Infection: A.C. Moreira, et al.; Int. J. Mol. Sci. 23, 269 (2022), Abstract;
Cytologic and molecular correlates of SARS-CoV-2 infection of the nasopharynx: G.J. Nuovo, et al.; Ann. Diagn. Pathol. 48, 151565 (2020), Application(s): IHC on nasopharyngeal swab cell preparations, Abstract; Full Text
Oral preconditioning of donors after brain death with calcineurin inhibitors vs. inhibitors of mammalian target for rapamycin in pig kidney transplantation: S.A. Dezfouli, et al.; Front. Immunol. 11, 1222 (2020), Abstract; Full Text
4-Phenylbutyric acid and rapamycin improved diabetic status in high fat diet/streptozotocin-induced type 2 diabetes through activation of autophagy: S.H. Gadallah, et al.; Arch. Physiol. Biochem. 127, 235 (2019), Application(s): Preparation of pancreatic tissue hogenates for Western blot, Abstract;
Diagnostic pathology of Alzheimer's disease from routine microscopy to immunohistochemistry and experimental correlations: G. Nuovo, et al.; Ann. Diagn. Pathol. 28, 24 (2017), Abstract;
False-positive results in diagnostic immunohistochemistry are related to horseradish peroxidase conjugates in commercially available assays: G. Nuovo; Ann. Diagn. Pathol. 25, 54 (2016), Application(s): Compare secondary HRP conjugate from Leica, Ventana, and Enzo in side-by-side comparison on the Leica BOND-MAX, Abstract;
