Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Fighting the epigenetic clock of aging

Epigenetics can be defined as the structural adaptation of chromosomal regions so as to register, signal or perpetuate altered activity states. A variety of chromatin-altering post-translational modifications to histone proteins including acetylation, methylation, and ubiquitinylation as well as direct modifications to DNA are known to turn gene transcription off or on. To date, methylation of DNA and acetylation/deacetylation of histone lysine residues have proven to be of greatest clinical significance amongst epigenetic post-translational modifications. The approval of anti-cancer drugs such as Vorinostat and Decitabine respectively targeting histone deacetylases (HDACs) and DNA methyltransferases (DNMTs) as well as pharma development pipelines targeting enzymes regulating post-translational modifications are evidence that reprogramming gene expression on an epigenetic level holds great clinical promise.


It is now understood that epigenetics are behind some of the genetic changes related to aging and that senescence could be delayed or inhibited by different approaches. Taking the example of a research article published in Genome Biology (2013), Steve Horvath from UCLA developed a model of age prediction based on a single type of epigenetic modification: DNA methylation at the 5 position of cytosine. This accurate age predictor was found to be independent of sex, tissue type, disease state and array platform as well as a strong indicator of the importance of epigenetics in aging. Histone deacetylases are a class of enzymes capable of removing acetyl groups from ε-N-acetyl lysine residues on histones. By acting on the latter and on chromatin structures, they have an influence on cell signaling, cell cycle, cell growth, cell death and disease. They also seem to be involved in the deacetylation of non-histone proteins, notably transcription factors whose function, activity and stability will be affected by these post-translational modifications. Recently, high expectations, notably from the personal care industry, have been placed on the anti-aging potential of modulators of histone deacetylases and most specifically NAD-dependent class III histone deacetylases (sirtuins) to promote homeostasis and preserve the levels of transcription and expression found in younger cells and younger organisms (A.M. Vaiserman et al., 2012).

Sirtuin-1, also referred to as SIRT1, is a NAD-dependent class III histone deacetylase and contributes to cellular regulation by protecting from stress events, favoring autophagy and augmenting longevity (P.D. Benech, et al., 2014). Dr. Chung and collaborators from Pusan National University and University of Texas HSC further investigated the mechanisms behind the positive effects of SIRT1 in skin fibroblasts. They demonstrated that SIRT1 protected skin cells from UVB-induced senescence both in vitro and in vivo. This protection was shown to change the transcriptional activity of FOXO3&aplha:, increase resistance to oxidative stress and suppress p53 acetylation and its transcriptional activity which has an effect on the cell cycle arrest triggered by UVB; thus making SIRT1 a very interesting biomarker for scientists working on human skin health and the development of skin care products. Several seminal studies have demonstrated the beneficial effects of the natural compound and SIRT1 activator, resveratrol, in preventing many diseases associated with aging. E.D. Lephart and colleagues from Brigham Young University decided to apply topically resveratrol on skin cells and noticed that it efficiently penetrated the human skin barrier and significantly stimulated the expression of SIRT1. It correlated with key events normally associated with healthy skin such as the increase expression of extracellular matrix proteins, tissue inhibitor of matrix metalloproteinases (TIMP1), several anti-oxidant and anti-aging biomarkers, and the inhibition of the expression of matrix metalloproteinases, aging biomarkers and pro-inflammatory biomarkers.

Altogether, these data suggest that SIRT1 and more specifically activators of SIRT1 could be hugely beneficial to both damaged and healthy but aging skin cells as a component of skin care products. Enzo Life Sciences offers a comprehensive portfolio for advancing your research in personal care such as the Fluor-de-Lys SIRT1 fluorometric drug discovery assay kit which is an ideal tool for the characterization of enzyme kinetics and the screening of modulators of SIRT1 activity. In addition, Enzo offers antibodies, enzymatic assays, immunoassays, live cell analysis assays and other small molecules, some of which are listed below:

Share this TechNote

Never miss a new TechNote!

Receive our TechNotes as soon as they are published.


Follow Us!

 
comments powered by Disqus

References:

  1. S. Horvath. DNA methylation age of human tissues and cell types. Genome Biol. (2013) 14: R115.
  2. A.M. Vaiserman, et al. Epigenetic drugs: a novel anti-aging strategy. Front. Genet. (2012) 3: 224.
  3. P.D. Benech, et al. From experimental design to functional gene networks: DNA microarray contribution to skin aging research. Int. J. Cosmet. Sci. (2014) 36: 516-26.
  4. K.W. Chung, et al. Molecular insights into SIRT1 protection against UVB-induced skin fibroblast senescence by suppression of oxidative stress and p53 acetylation. J. Gerontol. A. Biol. Sci. Med. Sci. (2014).
  5. E.D. Lephart, et al. Resveratrol: influences on gene expression in human skin. J. Funct. Foods. (2014) 10: 377-84.

Related Products

5-Hydroxymethylcytosine DNA ELISA kit 

Highly sensitive, rapid ELISA kit to quantify 5-hydroxymethylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-225-0001 96 wells 495.00 USD
Do you need bulk/larger quantities?
 

AROS (human) polyclonal antibody (AT135) 

Purified from rabbit serum., WB | Print as PDF
 
ALX-210-961-C100 100 µg 374.00 USD
Do you need bulk/larger quantities?
 

Piceatannol 

Syk inhibitor. SIRT1 activator.
10083-24-6, Synthetic., ≥98% (HPLC (UV)) | Print as PDF
 
ALX-270-202-M001 1 mg 40.00 USD
 
ALX-270-202-M005 5 mg 69.00 USD
 
ALX-270-202-M010 10 mg 122.00 USD
 
ALX-270-202-M050 50 mg 454.00 USD
Do you need bulk/larger quantities?
 

Butein 

Tyrosine kinase inhibitor
487-52-5, ≥98% (HPLC) | Print as PDF
 
ALX-350-246-M010 10 mg 215.00 USD
Do you need bulk/larger quantities?
 

Quercetin . dihydrate 

Antioxidant flavonoid
6151-25-3, Isolated from Sophora japonica L., ≥95% (HPLC) | Print as PDF
 
ALX-385-001-G005 5 g 37.00 USD
 
ALX-385-001-G025 25 g 59.00 USD
Do you need bulk/larger quantities?
 

SCREEN-WELL® Epigenetics library 

HTS | Print as PDF
 
BML-2836-0500 1 Library 500 µl/well Inquire for pricing
 
BML-2836-0100 1 Library 100 µl/well Inquire for pricing
Do you need bulk/larger quantities?
 

SCREEN-WELL® Natural Product library 

HTS | Print as PDF
 
BML-2865-0500 1 Library 500 µl/well Inquire for pricing
 
BML-2865-0100 1 Library 100 µl/well Inquire for pricing
Do you need bulk/larger quantities?
 

FLUOR DE LYS® HDAC fluorometric activity assay kit 

First-to-market by the leader in Epigenetics research tools
Fluorescent detection, HTS, Activity assay | Print as PDF
 
BML-AK500-0001 96 wells 507.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS® HDAC fluorometric cellular activity assay kit 

Monitor HDAC activity in cell culture using cell-permeable FLUOR DE LYS® substrate
Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
 
BML-AK503-0001 96 wells 468.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS®-Green HDAC fluorometric activity assay kit 

Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
 
BML-AK530-0001 96 wells 424.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS® SIRT1 fluorometric drug discovery assay kit 

First-to-market by the leader in Epigenetics research tools
Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
 
BML-AK555-0001 96 wells 676.00 USD
Do you need bulk/larger quantities?
 

Resveratrol 

Antioxidant. SIRT1 activator.
501-36-0, ≥98% (HPLC) | Print as PDF
 
BML-FR104-0100 100 mg 60.00 USD
 
BML-FR104-0500 500 mg 256.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS® deacetylase substrate 

Highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases.
≥95% (HPLC) | Print as PDF
 
BML-KI104-0050 50 µl 170.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS® -H4-AcK16 deacetylase substrate 

≥95% | Print as PDF
 
BML-KI174-0005 0.5 µmol 201.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS®-SIRT1 deacetylase substrate 

≥95% (HPLC) | Print as PDF
 
BML-KI177-0005 0.5 µmol 210.00 USD
Do you need bulk/larger quantities?
 

FLUOR DE LYS®-HDAC8 deacetylase substrate 

A fluorogenic, diacetylated peptide substrate for HDAC8 (histone deacetylase-8).
≥95% | Print as PDF
 
BML-KI178-0005 0.5 µmol 201.00 USD
Do you need bulk/larger quantities?
 

SIRT1 (human) polyclonal antibody 

Purified from rabbit serum., IHC (PS), WB | Print as PDF
 
BML-SA427-0100 100 µl 329.00 USD
Do you need bulk/larger quantities?
 

SIRT1 (human), (recombinant) (His-tag) 

Highly active
Produced in E. coli. Recombinant SIRT1 from human cDNA containing an N-terminal His-tag. | Print as PDF
 
BML-SE239-0100 100 U 351.00 USD
Do you need bulk/larger quantities?
 

EPIXTRACT® Total Histone Extraction Kit (High Throughput) 

Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.
Print as PDF
 
ENZ-45013-0002 2x96 Reactions 278.00 USD
Do you need bulk/larger quantities?
 

EPIXTRACT® Nuclear Protein Isolation Kit II (nucleic acid-free) 

Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.
Print as PDF
 
ENZ-45015-0100 100 Reactions 209.00 USD
Do you need bulk/larger quantities?
 

DNMT Colorimetric Drug Discovery Kit 

Rapid, sensitive, and simple colorimetric DNMT activity/inhibition kit.
Colorimetric detection | Print as PDF
 
ENZ-45017-0001 96 Reactions 731.00 USD
Do you need bulk/larger quantities?
 

EPIXTRACT® DNA Isolation Kit for Plasma/Serum 

Fastest procedure available to safely isolate DNA from plasma/serum.
Print as PDF
 
ENZ-45018-0050 50 Reactions 169.00 USD
 
ENZ-45018-0100 100 Reactions 248.00 USD
Do you need bulk/larger quantities?
 

CYTO-ID® Autophagy detection kit 

A no-transfection, quantitative assay for monitoring autophagy in live cells
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51031-0050 50 tests 215.00 USD
 
ENZ-51031-K200 200 tests 441.00 USD
Do you need bulk/larger quantities?
 

Cellular senescence activity assay 

Highly reproducible SA-β-gal activity kit
Fluorescent detection, HTS | Print as PDF
 
ENZ-KIT129-0120 120 assays 507.00 USD
Do you need bulk/larger quantities?
 

Cellular senescence live cell analysis assay 

Simple assay to quantify senescence-associated β-galactosidase activity in live cells using flow cytometry
Flow Cytometry | Print as PDF
 
ENZ-KIT130-0010 10 assays 308.00 USD
Do you need bulk/larger quantities?
 

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,