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5-Hydroxymethylcytosine DNA ELISA kit

Highly sensitive, rapid ELISA kit to quantify 5-hydroxymethylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
 
ADI-900-225-0001 96 wells 573.00 USD
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  • Rapid - fully quantitative results in < 3 hours
  • Convenient – user-friendly alternative to mass spectrometry
  • Simple – efficient and well-established protocol
  • High-throughput – 96 well format ideal for global detection, tissue-specific quantitation, high-throughput compound screening, and more
The 5-Hydroxymethylcytosine DNA ELISA kit is a complete, colorimetric, immunometric immunoassay kit for the quantitative determination of 5-hydroxymethylcytosine in DNA  with results in just 3 hours.
5-Hydroxymethylcytosine DNA ELISA kit Mass Spectroscopy vs ELISA
DNA Mass Spectroscopy versus ELISA analysis: The 5-hmC DNA ELISA Kit (prod. no. ADI-900-225) quantifies 5-hmC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. DNA samples include: brain, kidney, liver, thymus, human embryonic stem cells and two carcinoma samples.
5-Hydroxymethylcytosine DNA ELISA kit Standard curve
5-Hydroxymethylcytosine DNA ELISA kit schematic
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5-Hydroxymethylcytosine DNA ELISA kit Mass Spectroscopy vs ELISA 5-Hydroxymethylcytosine DNA ELISA kit Standard curve 5-Hydroxymethylcytosine DNA ELISA kit schematic

Product Details

Alternative Name:5-hmC
 
Sensitivity:<0.02% 5-hydroxymethylcytosine DNA per 100ng input DNA (range 0.03-0.55% (100 ng/µl))
 
Assay Time:~3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of species independent 5-hydroxymethylcytosine DNA in a variety of DNA samples.
 
Wavelength:450 nm
 
Species reactivity:Species independent
 
Use/Stability:Store all components at 4°C, except antibody and control set should be stored at -20°C, while the DNA HRP antibody should be stored at -80°C.
 
Shipping:Shipped on Blue Ice
 
Contents:Microtiter Plate, Coating Buffer, ELISA Buffer, Primary Antibody, Antibody-conjugate,  HRP Developer, Controls
 
Scientific Background:The 5-methylcytosine (5-mC) modification of DNA in epigenetic regulation has been well studied over the last several decades. However, the role of the so-called “sixth base”, 5-hydroxymethylcytosine (5-hmC), has yet to be defined. Recent studies have associated 5-hmC patterns to transcriptional regulation of genes, which may contribute to normal and disease states of organisms.
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 

Product Literature References

Atm deficiency in the DNA polymerase β null cerebellum results in cerebellar ataxia and Itpr1 reduction associated with alteration of cytosine methylation: J. Kim, et al.; Nucleic Acids Res. 48, 3678 (2020), Abstract; Full Text

General Literature References

Alterations of the lung methylome in allergic airway hyper-responsiveness: R.Y. Cheng, et al.; Environ. Mol. Mutagen. 55, 244 (2014), Abstract;

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