Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

5-Hydroxymethylcytosine DNA ELISA kit

Highly sensitive, rapid ELISA kit to quantify 5-hydroxymethylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
 
ADI-900-225-0001 96 wells 520.00 USD
Do you need bulk/larger quantities?
 
  • Rapid - fully quantitative results in < 3 hours
  • Convenient – user-friendly alternative to mass spectrometry
  • Simple – efficient and well-established protocol
  • High-throughput – 96 well format ideal for global detection, tissue-specific quantitation, high-throughput compound screening, and more
The 5-Hydroxymethylcytosine DNA ELISA kit is a complete, colorimetric, immunometric immunoassay kit for the quantitative determination of 5-hydroxymethylcytosine in DNA  with results in just 3 hours.
5-Hydroxymethylcytosine DNA ELISA kit Mass Spectroscopy vs ELISA
DNA Mass Spectroscopy versus ELISA analysis: The 5-hmC DNA ELISA Kit (prod. no. ADI-900-225) quantifies 5-hmC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. DNA samples include: brain, kidney, liver, thymus, human embryonic stem cells and two carcinoma samples.
5-Hydroxymethylcytosine DNA ELISA kit Standard curve
5-Hydroxymethylcytosine DNA ELISA kit schematic
Please mouse over
5-Hydroxymethylcytosine DNA ELISA kit Mass Spectroscopy vs ELISA 5-Hydroxymethylcytosine DNA ELISA kit Standard curve 5-Hydroxymethylcytosine DNA ELISA kit schematic

Product Details

Alternative Name:5-hmC
 
Sensitivity:<0.02% 5-hydroxymethylcytosine DNA per 100ng input DNA (range 0.03-0.55% (100 ng/µl))
 
Assay Time:~3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of species independent 5-hydroxymethylcytosine DNA in a variety of DNA samples.
 
Wavelength:450 nm
 
Species reactivity:Species independent
 
Use/Stability:Store all components at 4°C, except antibody and control set should be stored at -20°C, while the DNA HRP antibody should be stored at -80°C.
 
Shipping:Shipped on Blue Ice
 
Contents:Microtiter Plate, Coating Buffer, ELISA Buffer, Primary Antibody, Antibody-conjugate,  HRP Developer, Controls
 
Scientific Background:The 5-methylcytosine (5-mC) modification of DNA in epigenetic regulation has been well studied over the last several decades. However, the role of the so-called “sixth base”, 5-hydroxymethylcytosine (5-hmC), has yet to be defined. Recent studies have associated 5-hmC patterns to transcriptional regulation of genes, which may contribute to normal and disease states of organisms.
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 

Product Literature References

Atm deficiency in the DNA polymerase β null cerebellum results in cerebellar ataxia and Itpr1 reduction associated with alteration of cytosine methylation: J. Kim, et al.; Nucleic Acids Res. 48, 3678 (2020), Abstract; Full Text

General Literature References

Alterations of the lung methylome in allergic airway hyper-responsiveness: R.Y. Cheng, et al.; Environ. Mol. Mutagen. 55, 244 (2014), Abstract;

Related Products

5-Methylcytosine DNA ELISA kit 

Streamlined, rapid ELISA kit to quantify 5-methylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-224A-0001 96 wells 520.00 USD
Do you need bulk/larger quantities?
 

5-Hydroxymethylcytosine polyclonal antibody 

ELISA, IP, Immunoblot | Print as PDF
 
ADI-905-904-0050 50 µg 237.00 USD
 
ADI-905-904-0200 200 µg 672.00 USD
Do you need bulk/larger quantities?
 

Absorbance 96 Plate Reader 

Personal, Compact Plate Reader
ELISA, Colorimetric detection | Print as PDF
 
ENZ-INS-A96 1 ea Inquire for pricing
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
Cytosine
By biological activity:
Cytosine Immunoassay kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Epigenetics
Epigenetics
Download as PDF

Catalogs
Immunoassay Kits
Immunoassay Kits
Download as PDF

All new literature pieces

Recommend this page

 
Keep in touch

©2021 Enzo Life Sciences, Inc.,