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5-Methylcytosine DNA ELISA kit

Streamlined, rapid ELISA kit to quantify 5-methylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
ADI-900-224A-0001 96 wells 619.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: ADI-900-224

  • Rapid – fully quantitative results in < 3 hours
  • Convenient – user-friendly alternative to mass spectrometry
  • Simple – efficient and well-established protocol
  • High-throughput – 96 well format ideal for global 5-methylcytosine detection in DNA
The 5-Methylcytosine DNA ELISA kit is a complete, colorimetric, indirect immunoassay kit for the quantitative determination of 5-methylcytosine in DNA with results in less than 3 hours.

Product Details

Alternative Name:5-mC
Sensitivity:~0.5% 5-methylcytosine per 100ng single-stranded DNA (range 5-100% (100 ng/µl))
Assay Time:<3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of species independent 5-methylcytosine DNA in a variety of DNA samples.
Wavelength:450 nm
Species reactivity:Species independent
Use/Stability:Store Box 1 at 4°C and Box 2 containing the antibody, conjugate, and controls at -20°C.
Shipping:Dry Ice and Blue Ice
Contents:Microtiter Plate, Coating Buffer, ELISA Buffer, Primary Antibody, Antibody-conjugate,  HRP Developer, Controls
Scientific Background:The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research.  To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated DNA immunoprecipitation.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Maternal behaviours and adult offspring behavioural deficits are predicted by maternal TNFα concentration in a rat model of neurodevelopmental disorders: H.G. Potter, et al.; Brain Behav. Immun. 108, 162 (2023), Abstract;
Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny: L. Serra, et al. ; Toxics 9, 318 (2021), Abstract;
Decreased KAT5 expression impairs DNA repair and induces altered DNA methylation in kidney podocytes: A. Hishikawa, et al.; Cell Rep. 26, 1318 (2019), Application(s): ELISA using DNA from isolated podocytes, Abstract;
Genetic variants involved in oxidative stress, base excision repair, DNA methylation, and folate metabolism pathways influence myeloid neoplasias susceptibility and prognosis: A.C. Gonçalves, et al.; Mol. Carcinog. 56, 130 (2017), Application(s): Genomic DNA from whole human blood, Abstract;

General Literature References

Nutri-epigenetics ameliorates blood-brain barrier damage and neurodegeneration in hyperhomocysteinemia: role of folic acid: A. Kalani, et al.; J. Mol. Neurosci. 52, 202 (2013), Abstract; Full Text
Temozolomide downregulates P-glycoprotein expression in glioblastoma stem cells by interfering with the Wnt3a/glycogen synthase-3 kinase/β-catenin pathway: C. Riganti, et al.; Neuro. Oncol. 15, 1502 (2013), Abstract;

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