Replaces Prod. #: ADI-900-224
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Rapid – fully quantitative results in < 3 hours
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Convenient – user-friendly alternative to mass spectrometry
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Simple – efficient and well-established protocol
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High-throughput – 96 well format ideal for global 5-methylcytosine detection in DNA
The 5-Methylcytosine DNA ELISA kit is a complete, colorimetric, indirect immunoassay kit for the quantitative determination of 5-methylcytosine in DNA with results in less than 3 hours.
Product Details
| Alternative Name: | 5-mC |
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| Sensitivity: | ~0.5% 5-methylcytosine per 100ng single-stranded DNA (range 5-100% (100 ng/µl)) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of species independent 5-methylcytosine DNA in a variety of DNA samples. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Species independent
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| Use/Stability: | Store Box 1 at 4°C and Box 2 containing the antibody, conjugate, and controls at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Microtiter Plate, Coating Buffer, ELISA Buffer, Primary Antibody, Antibody-conjugate, HRP Developer, Controls |
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| Scientific Background: | The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research. To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated DNA immunoprecipitation. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny: L. Serra, et al. ; Toxics
9, 318 (2021),
Abstract;
Decreased KAT5 expression impairs DNA repair and induces altered DNA methylation in kidney podocytes: A. Hishikawa, et al.; Cell Rep.
26, 1318 (2019),
Application(s): ELISA using DNA from isolated podocytes,
Abstract;
Genetic variants involved in oxidative stress, base excision repair, DNA methylation, and folate metabolism pathways influence myeloid neoplasias susceptibility and prognosis: A.C. Gonçalves, et al.; Mol. Carcinog.
56, 130 (2017),
Application(s): Genomic DNA from whole human blood,
Abstract;
General Literature References
Nutri-epigenetics ameliorates blood-brain barrier damage and neurodegeneration in hyperhomocysteinemia: role of folic acid: A. Kalani, et al.; J. Mol. Neurosci.
52, 202 (2013),
Abstract;
Full Text
Temozolomide downregulates P-glycoprotein expression in glioblastoma stem cells by interfering with the Wnt3a/glycogen synthase-3 kinase/β-catenin pathway: C. Riganti, et al.; Neuro. Oncol.
15, 1502 (2013),
Abstract;
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