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SIRT1 (human) polyclonal antibody

BML-SA427-0100 100 µl 338.00 USD
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Product Specification

Alternative Name:Sirtuin-1
Immunogen:Recombinant human SIRT1 (sirtuin 1).
UniProt ID:Q96EB6
Source:Purified from rabbit serum.
Species reactivity:Human
Specificity:Recognizes recombinant and native SIRT1.
Crossreactivity:No cross-reactivity observed with recombinant human sirtuins SIRT2, SIRT3 or SIRT5.
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band pf ~115 kDa by Western blot. Calculated molecular weight of the full-length protein is 82 kDa.
Purity Detail:Protein A-affinity purified.
Formulation:Liquid. In PBS, pH 7.4.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
SIRT1 (human) polyclonal antibody Western blot
Western blot analysis of recombinant human sirtuins, Jurkat cells and HeLa nuclear extract. The nitrocellulose was probed with anti- SIRT1 at 1:1000. The secondary antibody was coupled to alkaline phosphatase and the blot was developed with BCIP/NBT. Arrows indicate positions of molecular weight markers. Lanes: S2, S3, S5 (SIRTs 2, 3 & 5, 50 ng each); J (150,000 Jurkat cells); H (18 μg HeLa nuclear extract); S1 (SIRT1- 5 ng, 0.5 ng).
SIRT1 (human) polyclonal antibody Immunohistochemistry
Immunohistochemistry staining of formalin fixed, paraffin-embedded human liver tumor tissue using SIRT1 antibody (Prod. no. BML-SA427) showing nuclear staining of hepatocytes.
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SIRT1 (human) polyclonal antibody Western blot SIRT1 (human) polyclonal antibody Immunohistochemistry

Product Literature References

Obesity depresses the anti-inflammatory HSP70 pathway, contributing to NAFLD progression: F.C. Di Nasco, et al.; Obesity 23, 120 (2015), Abstract;
Expression of silent mating type information regulator 2 homolog 1 and its role in human intervertebral disc cell homeostasis: Z. Zhang, et al.; Arthritis Res. Ther. 13, R200 (2011), Application(s): Immunofluorescence, Abstract; Full Text

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