| ENZ-KIT129-0120 | 120 assays | 595.00 USD |  |
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| Applications: | Fluorescent detection, HTS |
| Application Notes: | For the determination of cellular senescence by measuring SA-β-gal activity using a fluorometric substrate. |
| Handling: | Store SA-β-gal substrate solution protected from light at -20°C. Store all other components at room temperature. |
| Shipping: | Ambient Temperature |
| Contents: | 2X Cell lysis buffer 2X Reaction buffer SA-β-gal substrate (20X) Stop solution |
| Scientific Background: | Normal primary cells proliferate in culture for a limited number of population doublings prior to undergoing terminal growth arrest and acquiring a senescent phenotype. This finite life span correlates with the age of the organism and with the life expectancy of the species from which the cells were obtained; such that the older the age or the shorter the life span, the less the ability of the cells to undergo population doubling. Senescent cells are characterized by an irreversible G1growth arrest involving the repression of genes that drive cell cycle progression and the upregulation of cell cycle inhibitors like p16INK4a, p53, and its transcriptional target, p21CIP1. They are resistant to mitogen-induced proliferation, and assume a characteristic enlarged, flattened morphology. Research into the pathways that positively regulate senescence and ways cells bypass senescence is therefore critical in understanding carcinogenesis. Normal cells have several mechanisms in place to protect against uncontrolled proliferation and tumorigenesis. Senescent cells show common biochemical markers such as expression of an acidic senescence-associated β-galactosidase (SA-β-gal) activity. While senescence has been characterized primarily in cultured cells, there is also evidence that it occurs in vivo. Cells expressing markers of senescence such as SA-β-gal have been identified in normal tissues. |
| UniProt ID: | P16278 |
| Regulatory Status: | RUO - Research Use Only |
ROS-ID® Total ROS/Superoxide detection kit |
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| Widely cited kit to detect total ROS and Superoxide in live cells by microscopy and flow cytometry applications | ||||||
| Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF | ||||||
| ENZ-51010 | 1 Kit | 320.00 USD | |
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Cellular senescence live cell analysis assay |
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| Simple assay to quantify senescence-associated β-galactosidase activity in live cells using flow cytometry | ||||||
| Flow Cytometry | Print as PDF | ||||||
| ENZ-KIT130-0010 | 10 assays | 382.00 USD | |
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ROS-ID® ROS/RNS detection kit |
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| Widely cited kit to measure reactive oxygen and nitrogen species in live cells by fluorescence microscopy | ||||||
| Fluorescence microscopy | Print as PDF | ||||||
| ENZ-51001-200 | 1 Kit | 352.00 USD | |
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Cell Counting Kit-8 |
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| Simple, fast and accurate assay to measure cell proliferation and cytotoxicity | ||||||
| Colorimetric detection | Print as PDF | ||||||
| ALX-850-039-0100 | 100 tests | 100.00 USD | |
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| ALX-850-039-KI01 | 500 tests | 216.00 USD | |
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| ALX-850-039-KI02 | 5x500 tests | 641.00 USD | |
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NUCLEAR-ID® Red cell cycle kit (GFP-CERTIFIED®) |
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| Convenient kit for studying cell cycle progression by various applications | ||||||
| Flow Cytometry, Fluorescence microscopy | Print as PDF | ||||||
| ENZ-51008-100 | 1 Kit | 434.00 USD | |
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ROS-ID® Hypoxia/Oxidative stress detection kit |
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| Widely cited kit for the simultaneous analysis of hypoxia and oxidative stress by microscopy and flow cytometry applications | ||||||
| Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF | ||||||
| ENZ-51042-0125 | 125 tests | 133.00 USD | |
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| ENZ-51042-K500 | 500 tests | 463.00 USD | |
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Proteasome ELISA kit |
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| The only commercially available kit for proteasome quantification. | ||||||
| ELISA | Print as PDF | ||||||
| BML-PW0575-0001 | 96 wells | 542.00 USD | |
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HSP70 High Sensitivity ELISA kit |
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| Only commercially available kit specifically validated and sensitive enough to quantify HSP70 (HSP72) in serum and plasma samples. | ||||||
| ELISA, Colorimetric detection | Print as PDF | ||||||
| ADI-EKS-715 | 96 wells | 702.00 USD | |
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Neutrophil elastase colorimetric drug discovery kit |
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| Colorimetric detection, HTS, Activity assay | Print as PDF | ||||||
| BML-AK497-0001 | 96 wells | 571.00 USD | |
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Matrix metalloproteinase (MMP) inhibitor profiling kit, fluorometric RED |
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| Fluorescent detection, HTS, Activity assay | Print as PDF | ||||||
| BML-AK308-0001 | 96 wells | 1,236.00 USD | |
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Matrix metalloproteinase-1 (MMP-1) fluorometric drug discovery kit, RED |
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| Fluorescent detection, HTS, Activity assay | Print as PDF | ||||||
| BML-AK301-0001 | 96 wells | 694.00 USD | |
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p53 (human) ELISA kit |
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| ELISA | Print as PDF | ||||||
| ALX-850-057-KI01 | 96 wells | 874.00 USD | |
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| Download as PDF | |
