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Neutrophil elastase colorimetric drug discovery kit

BML-AK497-0001 96 wells 588.00 USD
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It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
Neutrophil elastase colorimetric drug discovery kit Fig1
Neutrophil elastase colorimetric drug discovery kit Fig2
Neutrophil elastase colorimetric drug discovery kit Fig3
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Neutrophil elastase colorimetric drug discovery kit Fig1 Neutrophil elastase colorimetric drug discovery kit Fig2 Neutrophil elastase colorimetric drug discovery kit Fig3

Product Details

Applications:Colorimetric detection, HTS
Activity assay
Application Notes:Screening of neutrophil elastase inhibitors.
Use/Stability:Substrates and inhibitor are in DMSO and are stable for up to 6 months. Neutrophil elastase activity decreases with time, even at -80°C.
Handling:Keep frozen at all times.
Shipping:Dry Ice
Long Term Storage:-80°C
Contents:Neutrophil elastase (human), (purified) (Prod. No. BML-SE284-9090)
pNA substrate (MeOSuc-AAPV-pNA) (Prod. No. BML-P213-9090)(60μl; 20mM in DMSO)
pNA calibration standard (p-nitroaniline) (Prod. No. BML-KI106)(1ml; 50μM in assay buffer)
Elastatinal (inhibitor) (Prod. No. BML-PI103-9090)(10μl; 20mM in DMSO)
Neutrophil elastase assay buffer (Prod. No. BML-KI581)(20ml; 100mM HEPES, pH 7.25, 500mM NaCl, 0.05% Tween-20; liquid in a screw-cap plastic bottle)
96-well microplate (Prod. No. BML-KI101)
UniProt ID:P08246
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Biochemical Pilot Study on Effects of Pomegranate Seed Oil Extract and Cosmetic Cream on Neurologically Mediated Skin Inflammation in Animals and Humans: A Comparative Observational Study: A.F. Hamouda, et al.; Molecules 28, 903 (2023), Abstract;
The methanol extract of Guettarda speciosa Linn. Ameliorates acute lung injury in mice: K.H. Kim, et al.; BMC Complement Med. Ther. 20, 40 (2020), Abstract; Full Text
Discovery and validation of 2-styryl substituted benzoxazin-4-ones as a novel scaffold for rhomboid protease inhibitors: P. Goel, et al.; Bioorg. Med. Chem. Lett. 28, 1417 (2018), Abstract;
In Vitro Evaluation of a Novel mRNA-Based Therapeutic Strategy for the Treatment of Patients Suffering from Alpha-1-Antitrypsin Deficiency: T. Michel, et al.; Nucleic Acid Ther. 25, 235 (2015), Abstract;
Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract: A.A. Karim, et al.; BMC Complement Altern. Med. 14, 381 (2014), Abstract; Full Text

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