| Alternative Name: | LAB72, CD28LG2 |
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| Host: | Rabbit |
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| Isotype: | IgG |
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| Immunogen: | Peptide corresponding to 17 amino acids near the center of human CD86. The immunogen is located within amino acids 160 - 210 of CD86. |
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| UniProt ID: | P42082 |
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| Species reactivity: | Human
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| Applications: | IF, IHC, WB
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| Purity Detail: | Affinity chromatography purified via peptide column. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response. Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia, suggesting that this pathway may be an attractive target for future cancer immunotherapy. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of CD86 in (A) Daudi and (B) Raji cell lysate with CD86 antibody at 1 µg/mL.
Immunohistochemistry of CD86 in human lymph node tissue with CD86 antibody at 2 µg/ml.
Immunofluorescence of CD86 in human lymph node tissue with CD86 antibody at 20 µg/ml. Red: CD86 Antibody (8681), Blue: DAPI staining.
