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SSEA-4 (human) recombinant monoclonal antibody (E2-F8)

A unique antibody targeting SSEA-4 that overcomes the challenges posed by the glycan nature of the antigen
 
ENZ-ABS858-0050 50 µg 425.00 USD
 
ENZ-ABS858-0100 100 µg 665.00 USD
 
ENZ-ABS858-0500 500 µg 1,520.00 USD
Do you need bulk/larger quantities?
 
SSEA-4 F8 antibody is an unconjugated recombinant rabbit monoclonal antibody to Stage-Specific Embryonic Antigen-4 (SSEA-4). Validated for ELISA, IHC-FR and immunoblotting applications.

Product Details

Alternative Name:Stage-specific embryonic antigen-4
 
Clone:E2-F8
 
Host:Rabbit
 
Isotype:IgG1
 
Immunogen:SSEA-4
 
Species reactivity:Human
 
Applications:ELISA, IHC, IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:ELISA (30-50 µg/ml)
Immunohistochemistry (paraffin sections 10-20 µg/ml). Antigen retrieval using a TrisEDTA buffer, pH9, is recommended.

Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Purified by Protein A or Protein G affinity chromatography
 
Endotoxin Content:<0.050 EU/mg as determined by Limulus Amebocyte Lysate (LAL) chromogenic endotoxin assay
 
Formulation:Liquid. In PBS pH 7.5
 
Handling:For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
 
Shipping:Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Stage-specific embryonic antigen-4 (SSEA-4) is a ganglioside which consists of a glycosphingolipid (GSL) containing a terminal sialic acid residue (N-acetylneuraminic acid). SSEA-4 expression changes both qualitatively and quantitatively during development, differentiation and in tumorigenesis. During human development, SSEA-4 is first observed on pluripotent cells of the inner cell mass and is subsequently lost upon differentiation.
SSEA-4 has been found to be over-expressed in a number of cancers and has been correlated with disease progression.
Studies have identified SSEA-4 as a novel marker to detect heterogeneous, invasive subpopulations of tumor cells. Importantly, it was also demonstrated that increased cell-surface SSEA-4 expression is associated with the loss of cell–cell interactions and the gain of a migratory phenotype, suggesting an important role of SSEA-4 in cancer invasion by influencing cellular adhesion to the extracellular matrix.
 
Regulatory Status:RUO - Research Use Only
 
SSEA-4 (human) recombinant monoclonal antibody (E2-F8) IHC
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SSEA-4 (human) recombinant monoclonal antibody (E2-F8) IHC

General Literature References

Carcinoembryonic antigen: W. Zimmermann; Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York 459 (2002),
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues: S. Hammarström; Semin. Cancer Biol. 9, 67 (1999), (Review), Abstract;

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