Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

3'Sulfo Lewis X recombinant monoclonal antibody (E2-B4)

Recombinant monoclonal antibody targeting 3’-Sulfo-Lewis X
 
ENZ-ABS859-0050 50 µg 566.00 USD
 
ENZ-ABS859-0100 100 µg 1,049.00 USD
 
ENZ-ABS859-0500 500 µg 2,655.00 USD
Do you need bulk/larger quantities?
 
The anti-3’Sulfo Lewis X is an unconjugated recombinant rabbit monoclonal antibody to 3’sulfo Lewis X. Validated for ELISA, IHC-FR and immunoblotting applications.

Product Details

Alternative Name:3-SLeX
 
Clone:E2-B4
 
Host:Rabbit
 
Isotype:IgG1
 
Immunogen:3'Sulfo-Lewis X
 
Species reactivity:Human
 
Applications:ELISA, IHC, IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:ELISA (20-50 µg/ml)
Immunohistochemistry (paraffin sections 10-40 µg/ml). Antigen retrieval using a TrisEDTA buffer, pH9, is recommended.

Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Purified by Protein A or Protein G affinity chromatography
 
Endotoxin Content:<0.050 EU/mg as determined by Limulus Amebocyte Lysate (LAL) chromogenic endotoxin assay
 
Formulation:Liquid. In PBS pH 7.5
 
Handling:For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
 
Shipping:Ambient Temperature
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:3’-Sulfo-Lewis X is a glycan epitope that has been reported to act as a ligand for the adhesion molecules E- and L-selectin.  The synthesis of 3-SLeX is mediated by the enzyme galactose-3-O-sulfotransferase (GAL3ST2), which is expressed in cell lines with high metastatic potential. Expression of 3-SLeX is therefore potentially relevant to the identification of tumours with poor prognosis and higher likelihood of progression and metastasis.
3-SLeX has been found to be expressed at high levels in the mucins from the lungs of Cystic Fibrosis patients. It has been shown that Pseudomonas Aeruginosa preferentially binds to this form of LeX, and that high levels of expression may be related to more serious infections and chronic colonisation of airway mucins in Cystic Fibrosis.
 
Regulatory Status:RUO - Research Use Only
 
3'Sulfo Lewis X recombinant monoclonal antibody (E2-B4) IHC
Please mouse over
3'Sulfo Lewis X recombinant monoclonal antibody (E2-B4) IHC

General Literature References

Carcinoembryonic antigen: W. Zimmermann; Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York 459 (2002),
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues: S. Hammarström; Semin. Cancer Biol. 9, 67 (1999), (Review), Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
SDS
Certificate of Analysis

RELATED PRODUCTS

By target:
3'Sulfo Lewis X
By biological activity:
3'Sulfo Lewis X Recombinant antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service