AMPIVIEW™ RNA probes are uniquely designed with the precision of targeted, sequence specific RNA probes, powered by Enzo’s LoopRNA ISH™ technology to deliver superior sensitivity. When AMPIVIEW™ RNA probes hybridize to nucleic acid targets, loops form exposing biotin or digoxigenin labels, making these probes not only the most sensitive means of detection, but adaptable with existing immunohistochemistry (IHC) detection solutions and chromogens. Additionally, morphology of the sample is preserved and the signal of the targeted biomarker can be seen under the light microscope.
Basic Research and Drug Discovery/Development
|
Clinical Research
|
What is in situ hybridization and what are the recent advancements with this indispensable technique?
2022 September 16 | Tags:
Molecular Biology,
Cancer,
We are Enzo!
What are the Differences between DNA and RNA Probes?
2019 May 10 | Tags:
Molecular Biology,
Successful Research Tips
10 Tips for Optimizing In Situ Hybridization (ISH)
2018 Mai 11 | Tags:
Molecular Biology,
Successful Research Tips
|
|
Detection of HPV 16/18 in SiHa cells (1-3 HPV integrated copies) with A. AMPIVIEW™ HPV 16/18 sense RNA probes to detect viral DNA only and B. AMPIVIEW™ HPV 16/18 antisense RNA probes to detect both viral DNA and viral RNA in cells.
|
|
|
AMPIVIEW™ SARS-CoV-2 RNA probes
|
AMPIVIEW™ SARS-CoV-2 RNA probes
|
Competitor SARS-CoV-2 RNA probes
|
|
|
 |
|
Pre-treatment is performed depending on the sample type:
|
Antigen Retrieval Reagent, pH 6 (10X) |
||||||
| IHC | Print as PDF | ||||||
| ENZ-ACC112-0100 | 100 ml | 154.00 USD |  |
|||
| Do you need bulk/larger quantities? | ||||||
Antigen Retrieval Reagent, pH 9 (10X) |
||||||
| IHC | Print as PDF | ||||||
| ENZ-ACC113-0100 | 100 ml | 149.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Proteinase K |
||||||
| PCR grade proteinase K used for molecular biology applications | ||||||
| 39450-01-6, Pichia pastoris | Print as PDF | ||||||
| ENZ-33801 | 2x5 mg | 77.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
IHC antigen retrieval reagent (citrate, pH 7.0) |
||||||
| IHC | Print as PDF | ||||||
| ADI-950-271-0500 | 500 ml | 123.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
IHC antigen retrieval reagent (citrate, pH 8.0) |
||||||
| IHC | Print as PDF | ||||||
| ADI-950-272-0500 | 500 ml | 146.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
IHC enzyme antigen retrieval reagent |
||||||
| IHC | Print as PDF | ||||||
| ADI-950-280-0015 | 15 ml | 181.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
|
AMPIVIEW™ RNA probes — Ready-to-Use ProbesAMPIVIEW™ RNA probes are designed with Enzo’s LoopRNA ISH™ technology that hybridize to a specific RNA, DNA or RNA/DNA target of interest. Every target probe contains biotin or digoxigenin tags that enable the associated target to be visualized in a specific color chromogens under the light microscope. |
AMPIVIEW™ Circ018 (AS) Dig RNA Probes (Mouse) Set |
||||||
| A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection lncRNA Circ018 in mouse or rat cells and tissues | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN180-2000 | 2 ml | 750.00 USD | |
|||
AMPIVIEW™ EGFR (AS) Dig RNA Probes Set |
||||||
| A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection EGFR RNA or DNA/RNA in cells and tissues | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN129-2000 | 2 ml | 750.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
AMPIVIEW™ GAPDH (AS) Dig RNA Probes Set |
||||||
| A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection GAPDH RNA or DNA/RNA in cells and tissues | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN127-2000 | 2 ml | 300.00 USD | |
|||
AMPIVIEW™ GAPDH and NSP Dig Control Probes Kit |
||||||
| Positive and negative control RNA probes labeled with digoxigenin for in situ hybridization (ISH) in cells and tissues. | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-KIT224-0040 | 40 tests | 500.00 USD | |
|||
AMPIVIEW™ HPV 6/11 RNA Probes Set |
||||||
| A mixture of digoxigenin-labeled HPV 6 and HPV 11-specific RNA probes for in situ hybridization | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN145-2000 | 2 ml | 515.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
AMPIVIEW™ HPV 16/18 RNA Probes Set |
||||||
| A mixture of digoxigenin-labeled RNA probes for the detection of HPV 16/18 for in situ hybridization | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN146-2000 | 2 ml | 515.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
AMPIVIEW™ HPV 31/33/51 RNA Probes Set |
||||||
| A mixture of digoxigenin-labeled RNA probes for the detection of HPV 31/33/51 for in situ hybridization. | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN147-2000 | 2 ml | 515.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
AMPIVIEW™ HPV High-Risk RNA Probes Set |
||||||
| A mixture of digoxigenin-labeled RNA probes for the detection of HPV 16/18/31/33/51 for in situ hybridization. | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN148-2000 | 2 ml | 618.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
AMPIVIEW™ NORAD (AS) Dig RNA Probes (Mouse) Set |
||||||
| A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection lncRNA NORAD in mouse or rat cells and tissues | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN178-2000 | 2 ml | 750.00 USD | |
|||
AMPIVIEW™ Ubiquitin (AS) Dig RNA Probes Set |
||||||
| A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection ubiquitin RNA or DNA/RNA in cells and tissues. | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN125-2000 | 2 ml | 300.00 USD | |
|||
AMPIVIEW™ Ubiquitin and NSP Dig Control Probes Kit |
||||||
| Positive and negative control RNA probes labeled with digoxigenin for in situ hybridization (ISH) in cells and tissues. | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-KIT223-0040 | 40 tests | 500.00 USD | |
|||
AMPIVIEW™ SARS-CoV-2 RNA Probes Set |
||||||
| A mitxture of biotin-labeled RNA probes for the in situ hybridization detection SARS-CoV-2 virus. | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN159-2000 | 2 ml | 515.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
AMPIVIEW™ SARS-CoV-2 RNA Probes Kit |
||||||
| AMPIVIEW™ SARS-CoV-2 RNA Probes kit comes, AMPIVIEW™ SARS-CoV-2 RNA Probes, powered by Enzo's LoopRNA ISH™ technology to deliver superior sensitivity with SAVIEW® PLUS detection system. | ||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-GEN158-0020 | 20 assays | 1,030.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
|
One-Step Detection SystemSAVIEW® PLUS AP or HRP Reagent is a ready-to-use s treptavidin-based nanopolymer reagent to ensure consistent and reproducible detection of biotinylated probes on tissue sections and cells. SAVIEW® PLUS one-step detection method for biotinylated probes enables faster staining procedures with significantly lower background. |
SAVIEW® PLUS AP Reagent |
||||||
| High sensitivity, low background, streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC111-0150 | 150 tests | 192.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
SAVIEW® PLUS HRP reagent |
||||||
| High sensitivity, low background streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC102-0150 | 150 tests | 192.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
|
Two-Step Detection SystemAnti-biotin or DIGX® anti-digoxigenin linker antibodies are used in combination with ready to-use biotin-free nanopolymer POLYVIEW® PLUS AP or HRP detection reagents for consistent and reproducible immunodetection of AMPIVIEW™ RNA probes. POLYVIEW® PLUS high intensity color development delivers sharp and crisp stainings. |
POLYVIEW® PLUS AP (anti-mouse) reagent |
||||||
| High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC114-0150 | 150 tests | 183.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
POLYVIEW® PLUS AP (anti-rabbit) reagent |
||||||
| High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC110-0150 | 150 tests | 183.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
POLYVIEW® PLUS HRP (anti-mouse) reagent |
||||||
| High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC104-0150 | 150 tests | 183.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
POLYVIEW® PLUS HRP (anti-rabbit) reagent |
||||||
| High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC103-0150 | 150 tests | 183.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) |
||||||
| Superior multiplex IHC kit for efficient detection of multiple antigens in a single tissue sample. | ||||||
| IHC | Print as PDF | ||||||
| ENZ-KIT181-0150 | 150 tests | 751.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Mouse anti-biotin linker (Ready-to-use) |
||||||
| ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-32893-6000 | 6 ml | 478.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Rabbit anti-biotin linker (Ready-to-use) |
||||||
| Partially purified IgG fraction of rabbit serum., ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-32892-6000 | 6 ml | 489.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
DIGX® Mouse anti-digoxigenin linker (Ready-to-Use) |
||||||
| Ready-to-use linker for ISH applications | ||||||
| Purified IgG fraction of mouse serum., ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ABS304-6000 | 6 ml | 473.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
DIGX® Rabbit anti-digoxigenin linker (Ready-to-Use) |
||||||
| Ready-to-use linker for ISH applications | ||||||
| Purified IgG fraction of rabbit serum., ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ABS303-6000 | 6 ml | 473.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
|
AMPIVIEW™ RNA Probes can be visualized and imaged with a conventional light microscope. |
HIGHDEF® DAB Chromogen/Substrate Set |
||||||
| Brown chromogen for use with HRP immunohistochemistry staining systems | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC105-0200 | 200 ml | 267.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HIGHDEF® Green AP Chromogen/Substrate |
||||||
| High definition IHC chromogen producing brilliant green color for AP-activated development. | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC130-0030 | 30 ml | 283.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HIGHDEF® black IHC chromogen (HRP) |
||||||
| Unique high definition black IHC chromogen for HRP-activated development. | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ADI-950-171-0030 | 30 ml | 216.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HIGHDEF® blue IHC chromogen (AP) |
||||||
| High definition IHC chromogen producing brilliant blue color for AP-activated development. | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ADI-950-150-0030 | 30 ml | 216.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HIGHDEF® blue IHC chromogen (HRP) |
||||||
| Unique high definition blue IHC chromogen for HRP-activated development. | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ADI-950-151-0030 | 30 ml | 216.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HIGHDEF® red IHC chromogen (AP) |
||||||
| High definition IHC chromogen producing brilliant red color for AP-activated development. | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ADI-950-140-0030 | 30 ml | 128.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HIGHDEF® yellow IHC chromogen (HRP) |
||||||
| Unique high definition yellow IHC chromogen for HRP-activated development. | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ADI-950-170-0030 | 30 ml | 221.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HIGHDEF® Hematoxylin |
||||||
| Ready-to-use histologic stain with enhancers | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC106-0100 | 100 ml | 61.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
HIGHDEF® IHC mount |
||||||
| Permanent, high stability mounting medium for high definition visualization of IHC. | ||||||
| IF, IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ADI-950-261-0030 | 30 ml | 80.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Which targets can AMPIVIEW™ probes detect?Our AMPIVIEW™ probes based on Enzo’s proprietary LoopRNA™ ISH technology offer outstanding, sensitivity for in situ hybridization, allowing chromogenic detection of nucleic acid targets with a sensitivity that is on par with standard PCR methods – but on tissue slides with all the benefits provided by spatial biology. AMPIVIEW™ probes can detect both DNA and RNA targets, regardless of whether these are genomic targets or expressed copies of RNA, including but not limited to genomic sequences, mRNA, cytosolic viral genomes, mitochondrial DNA. |
How sensitive are AMPIVIEW™ probes based on LoopRNA™ ISH technology?AMPIVIEW™ probes display extraordinarily high sensitivity, allowing successful chromogenic detection and a strong, easy to visualize staining on par with the sensitivity of standard PCR. |
How are AMPIVIEW™ probes labeled?The outstanding sensitivity of our AMPIVEW™ probes for in situ hybridization is rooted in Enzo’s proprietary LoopRNA™ ISH technology, which utilizes labeled loops with a high spatial density of label molecules, which are intermittently located throughout the specific hybridization sequence of each probe. These labeled loops can be labeled with either biotin or digoxigenin. This LoopRNA™ ISH technology results on sensitivity on par with standard PCR methods. |
What is the ideal probe length for AMPIVIEW™ probes?Sense-probes for genomic targets ideally utilize about 3kb of hybridization sequence, while antisense expression probes, which target DNA and RNA, utilize 2kb of hybridization sequence or less, depending on the level of expression of the respective mRNA. |
How are AMPIVEW™ probes detected?Our AMPIVIEW™ probes are labeled with either biotin or digoxigenin. For biotin-labeled probes, we developed both streptavidin-based detection with our SAVIEW® PLUS detection reagents as well as immunogenic detection with anti-biotin linker antibodies and our POLYVIEW® PLUS detection solutions. Digoxigenin-detection is achieved with anti-digoxigenin linker antibodies in combination with our POLYVIEW® PLUS detection solutions. Regardless of the utilized detection reagent, our HIGHDEF® chromogens offer crisp color chromogenic staining in a broad palette of colors. Of course, AMPIVIEW™ probes can be detected with a broad variety of third-party detection solutions for biotin or digoxigenin labels, including fluorescent detection. |
Which tissue preparation does AMPIVIEW™ require?Our AMPIVIEW™ probes are typically optimized for formalin-fixed, paraffin-embedded tissue samples and suited for a wide variety of tissue types. Additionally, they are perfectly suited for cryopreserved tissue sections as well, although we do not typically validate this type of tissue specimen in-house. Due to the relative standard and very flexible protocol for in situ hybridization with our AMPIVIEW™ probes, they are theoretically adaptable to a wide variety of cell and tissue specimen and histological techniques, regardless of species origin or fixation and embedding method, as long as the nucleic acids in the sample are preserved. |
What is the volume required for hybridization?We typically provide our AMPIVIEW™ probes as 2-mL ready-to-use solutions for 20-40 tests. A volume of 50-100 µL per slide has shown to be sufficient for a broad variety of applications and liquid handling procedures for manual and automated workflows, regardless of whether liquid is contained by a hydrophobic barrier, under a cover (such as glass cover slips), or protected from evaporation by an oily film. Depending on the utilized procedure, size of the tissue sections and other variables, significantly smaller volumes might be sufficient. |
Does AMPIVIEW™ require proprietary reagents?Our biotin- or digoxigenin-labeled AMPIVIEW™ probes can be detected with a broad variety of detection solutions designed to detect the respective labels. AMPIVIEW™-driven chromogenic in situ hybridization provides outstanding results with our SAVIEW® PLUS and POLYVIEW® PLUS detection solutions in combination with our HIGHDEF® chromogens for crisp, strong, and colorful staining that eliminate data ambiguity. However, AMPIVIEW™ probes can be combined with any ISH or IHC solution suitable for biotin or digoxigenin, including fluorescent detection. |
Is AMPIVIEW™ compatible with autostainer instrumentation?The AMPIVIEW™ workflow allows a high degree of flexibility regarding the respective workflow and the degree of automation. All our AMPIVEW™ products are suitable for manual workflows and a variety of open automated staining instruments. Depending on the autostainer platform, our technical support might provide useful help for the optimization up to established protocols. However, validation of any given workflow is required to maximize results. |
Do you provide positive or negative control probes?Depending on the target and tissue of choice, we can provide negative and positive control probes. For general purposes, we recommend to use a universal negative scrambled control probe and one of our catalogue probes of ubiquitously expressed targets, such as GAPDH or ubiquitin. |
Is AMPIVIEW™ in situ hybridization influenced by single point mutations?No. Due to the nature of the AMPIVIEW™ probes and the length of the hybridization area, single point mutations do not influence the signal obtained with Enzo’s AMPIVIEW™ probes. This makes our AMPIVIEW™ probes a great tool to analyze gene targets and expression irrelevant of minor variations, and for example allows the simultaneous detection of virus variants. |
Can AMPIVIEW™ probes be used to detect genomic translocation?Dual probes utilizing biotin and digoxigenin labels may potentially be used to detect translocation, if the spatial separation between both probe locations is large enough. However, procedures will require careful validation by the end user. Break point localization will be approximate due to the nature of the AMPIVIEW™ probes. |
What is the Quality Control (QC) process for AMPIVIEW™ probes?The purity of our AMPIVIEW™ probes is determined by A260/280 spectroscopy and gel electrophoresis. Performance is analyzed by dot blot hybridization tests. In situ hybridization on cell or tissue is optional. |
|
Understanding the histopathology of COVID-19 has proven to be a major gateway to understanding the molecular mechanisms through which SARS-CoV-2 manifests disease. The group of Dr. Nuovo has contributed significantly to understanding the pathophysiology of severe COVID-19 and utilized Enzo’s AMPIVIEW SARS-CoV-2 RNA probes kit in the process. He will present the highlights of his group’s findings in Enzo’s webinar series. Amongst them: 1. Detection of SARS-CoV-2 RNA in situ shows that productive infection predominates in the nasopharynx and lung; 2. Detection of SARS-CoV-2 proteins (nucleocapsid, spike, envelope and matrix) show that spike is avidly endocytosed via the vascular system in several sites, including the brain and heart; viral RNA is rarely evident in these sites; 3. Correlation of the host response to the in situ localization of SARS-CoV-2 RNA and spike protein shows that each are associated with increased expression of proinflammatory proteins including TNFa and IL6 as well as complement activation.
Main Target Audience: Pathophysiologists; Clinical Laboratories; Hospitals; Histologists; Virologists - Researchers interested in the following topics: Coronavirus, in situ Hybridization (ISH); Antibodies; Clinical Diagnostics; DNA/Protein Labeling, HPV, Immunohistochemistry (IHC), Immunology, Inflammation, Pathology, Virology. Why should you attend?: Whether you are a scientist, a student, a lab technician, or a curious citizen, this webinar will provide fascinating mechanistic insight into the pathophysiology of severe COVID-19, while simultaneously providing a wealth of practical knowledge on the laboratory technologies utilized in detecting viral RNA and proteins, especially in situ hybridisation and immunohistochemistry. Presented by: Dr. Gerard J. Nuovo
Topic: Clinical |
 |
