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AMPIVIEW™ RNA probes

Powered by Enzo’s LoopRNA ISH™ Technology


Visualize the expression and spatial biology of target genes with ease


AMPIVIEW™ RNA probes are uniquely designed with the precision of targeted, sequence specific RNA probes, powered by Enzo’s LoopRNA ISH™ technology to deliver superior sensitivity. When AMPIVIEW™ RNA probes hybridize to nucleic acid targets, loops form exposing biotin or digoxigenin labels, making these probes not only the most sensitive means of detection, but adaptable with existing immunohistochemistry (IHC) detection solutions and chromogens. Additionally, morphology of the sample is preserved and the signal of the targeted biomarker can be seen under the light microscope.

AMPIVIEW™ RNA probes Benefits


  • Detection of unique nucleic acid targets (DNA/RNA) down to a single cell level
  • High sensitivity and reliability
  • Protocols for formalin-fixed, paraffin-embedded (FFPE) tissues and cells
  • Mild protocols to preserve tissue morphology
  • End-user can use existing ISH set ups and protocols
  • Optimized with Enzo’s cost-effective IHC/ISH detection portfolio
  • Flexible products ready for any workflow (Manual or Automated)

Applications

Sensitive, adaptable, reliable, and cost-effective solutions


Basic Research and Drug Discovery/Development

  • Physiological transcription analysis
  • Disease model studies
  • Biomarkers research
  • Target identification and validation
  • Early preclinical drug discovery analysis
  • Therapeutics cell response
  • Companion diagnostic research and development
  • Spatial Biology

Clinical Research

  • Pathogen identification
  • Biomarker detection
  • Biomarker validation

AMPIVIEW™ RNA probes can detect copies
of DNA with sense probes
and DNA and RNA with antisense probes


Detection of HPV 16/18 in SiHa cells (1-3 HPV integrated copies) with A. AMPIVIEW™ HPV 16/18 sense RNA probes to detect viral DNA only and B. AMPIVIEW™ HPV 16/18 antisense RNA probes to detect both viral DNA and viral RNA in cells.

Enzo’s AMPIVIEW™ RNA Probes produce high signal and low background


Enzo HPV High Risk antisense probes (RNA)


Red stain = positive signal

Leading competitor HPV High Risk probes


Brown stain = positive signal

Cervical Squamous Cervical Carcinoma - HPV strong expressing

"Loop-RNA probes do not require lengthy protocol to aid the formation of signal structures required in branched DNA probe technology that in turn, tend to increase background."


Dr. Nuovo, Ohio State University

Read the full paper: Nuovo GJ et Al. Appl Immunohistochem Mol Morphol. 2022 Feb 1;30(2):83-90.

AMPIVIEW™ RNA Probes are highly specific


Detection of SARS-CoV-2 in Lung Tissue


Detection of SARS-CoV-2 RNA. A and B, show the in situ hybridization SARS-CoV-2 RNA in fatal COVID-19 pneumonia with Leading Competitor and Enzo’s probes. The Competitor probes also signal macrophages (circles, A).
Nuovo GJ et Al. Appl Immunohistochem Mol Morphol. 2022 Feb 1;30(2):83-90.




SARS-CoV-2-negative tissues show no background staining with Enzo’s AMPIVIEW™ RNA probes

AMPIVIEW™ SARS-CoV-2 RNA probes

AMPIVIEW™ SARS-CoV-2 RNA probes

Competitor SARS-CoV-2 RNA probes




"I tested some normal brain and placenta as negative controls for SARS-CoV-2 RNA by ISH with the ACD RNAscope™ and Enzo AMPIVIEW™ probes. Note the clean results with the Enzo’s probe. And, also, note the background (red blood cells) with the ACD probes"


Dr. Nuovo, Ohio State University

Chromogenic in situ Hybridization E-book

  • An Introduction to Chromogenic in situ Hybridization (CISH)
  • Top 10 Tips for Successful CISH
  • Methods to Generate Probes
  • Biotin or Digoxigenin
  • DNA vs RNA Probes
  • Sample Preparation
  • Hybridization
  • Controlling Non-specific Background with Blocking
  • Methods of Detection for CISH
  • Single vs Dual Staining
  • Common Counterstaining for CISH
  • Applications of CISH
  • FAQs on CISH

AMPIVIEW™ RNA Probes in situ Hybridization Assay Workflow

From sample preparation to visualization

Sample Preparation

Optimal sample preparation is the foundation of outstanding results with AMPIVIEW™ RNA Probes Assay.
  • Formalin-fixed, paraffin-embedded (FFPE) tissue sample
  • Cryopreserved tissue sample
  • Cells — cultured, cytology samples, etc

Permeabilize and Denature

Pre-treatment is performed depending on the sample type:
  • Fixation of cells or tissue
  • Deparaffinization (FFPE tissues)
  • Antigen retrieval
  • Enzymatic permeabilization

Antigen Retrieval Reagent, pH 6 (10X) 

IHC | Print as PDF
 
ENZ-ACC112-0100 100 ml Inquire for pricing
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Antigen Retrieval Reagent, pH 9 (10X) 

IHC | Print as PDF
 
ENZ-ACC113-0100 100 ml Inquire for pricing
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Proteinase K 

PCR grade proteinase K used for molecular biology applications
39450-01-6, Pichia pastoris | Print as PDF
 
ENZ-33801 2x5 mg 67.00 USD
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IHC antigen retrieval reagent (citrate, pH 7.0) 

IHC | Print as PDF
 
ADI-950-271-0500 500 ml Inquire for pricing
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IHC antigen retrieval reagent (citrate, pH 8.0) 

IHC | Print as PDF
 
ADI-950-272-0500 500 ml Inquire for pricing
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IHC enzyme antigen retrieval reagent 

IHC | Print as PDF
 
ADI-950-280-0015 15 ml Inquire for pricing
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Hybridize

AMPIVIEW™ RNA probes — Ready-to-Use Probes

AMPIVIEW™ RNA probes are designed with Enzo’s LoopRNA ISH™ technology that hybridize to a specific RNA, DNA or RNA/DNA target of interest. Every target probe contains biotin or digoxigenin tags that enable the associated target to be visualized in a specific color chromogens under the light microscope.

AMPIVIEW™ HPV 6/11 RNA Probes Set 

A mixture of digoxigenin-labeled HPV 6 and HPV 11-specific RNA probes for in situ hybridization
ISH (in situ hybridization) | Print as PDF
 
ENZ-GEN145-2000 2 ml Inquire for pricing
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AMPIVIEW™ HPV 16/18 RNA Probes Set 

A mixture of digoxigenin-labeled RNA probes for the detection of HPV 16/18 for in situ hybridization
ISH (in situ hybridization) | Print as PDF
 
ENZ-GEN146-2000 2 ml Inquire for pricing
Do you need bulk/larger quantities?
 

AMPIVIEW™ HPV 31/33/51 RNA Probes Set 

A mixture of digoxigenin-labeled RNA probes for the detection of HPV 31/33/51 for in situ hybridization.
ISH (in situ hybridization) | Print as PDF
 
ENZ-GEN147-2000 2 ml Inquire for pricing
Do you need bulk/larger quantities?
 

AMPIVIEW™ HPV High-Risk RNA Probes Set 

A mixture of digoxigenin-labeled RNA probes for the detection of HPV 16/18/31/33/51 for in situ hybridization.
ISH (in situ hybridization) | Print as PDF
 
ENZ-GEN148-2000 2 ml Inquire for pricing
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AMPIVIEW™ SARS-CoV-2 RNA Probes Set 

A mitxture of biotin-labeled RNA probes for the in situ hybridization detection SARS-CoV-2 virus.
ISH (in situ hybridization) | Print as PDF
 
ENZ-GEN159-2000 2 ml Inquire for pricing
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AMPIVIEW™ SARS-CoV-2 RNA Probes Kit 

AMPIVIEW™ SARS-CoV-2 RNA Probes kit comes, AMPIVIEW® SARS-CoV-2 RNA Probes, powered by Enzo's LoopRNA ISH™ technology to deliver superior sensitivity with SAVIEW® PLUS detection system.
ISH (in situ hybridization) | Print as PDF
 
ENZ-GEN158-0020 20 assays Inquire for pricing
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Custom Probes

Enzo can design probes for practically ANY gene in ANY genome to be used in ANY tissue or cells.
To order or inquire about custom probes, please fill in the from and a product specialist will contact you.

Detect and Develop

AMPIVIEW™ RNA Probes can be detected with Enzo’s IHC/ISH Detection Kits and chromogens.

One-Step Detection System

SAVIEW® PLUS AP or HRP Reagent is a ready-to-use s treptavidin-based nanopolymer reagent to ensure consistent and reproducible detection of biotinylated probes on tissue sections and cells. SAVIEW® PLUS one-step detection method for biotinylated probes enables faster staining procedures with significantly lower background.

SAVIEW® PLUS AP Reagent 

High sensitivity, low background, streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC111-0150 150 tests Inquire for pricing
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SAVIEW® PLUS HRP reagent 

High sensitivity, low background streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC102-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

Two-Step Detection System

Anti-biotin or DIGX® anti-digoxigenin linker antibodies are used in combination with ready to-use biotin-free nanopolymer POLYVIEW® PLUS AP or HRP detection reagents for consistent and reproducible immunodetection of AMPIVIEW™ RNA probes. POLYVIEW® PLUS high intensity color development delivers sharp and crisp stainings.
Stunning results can be achieved when combining SAVIEW® PLUS or POLYVIEW® PLUS reagents with Enzo’s unrivaled selection of HIGHDEF® chromogens (see list of colors below).

POLYVIEW® PLUS AP (anti-mouse) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC114-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS AP (anti-rabbit) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC110-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS HRP (anti-mouse) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC104-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS HRP (anti-rabbit) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC103-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) 

Superior multiplex IHC kit for  efficient detection of multiple antigens in a single tissue sample.
IHC | Print as PDF
 
ENZ-KIT181-0150 150 tests Inquire for pricing
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Mouse anti-biotin linker (Ready-to-use) 

ISH (in situ hybridization) | Print as PDF
 
ENZ-32893-6000 6 ml 423.00 USD
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Rabbit anti-biotin linker (Ready-to-use) 

Partially purified IgG fraction of rabbit serum., ISH (in situ hybridization) | Print as PDF
 
ENZ-32892-6000 6 ml 437.00 USD
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DIGX® Mouse anti-digoxigenin linker (Ready-to-Use) 

Ready-to-use linker for ISH applications
Purified IgG fraction of mouse serum., ISH (in situ hybridization) | Print as PDF
 
ENZ-ABS304-6000 6 ml 426.00 USD
Do you need bulk/larger quantities?
 

DIGX® Rabbit anti-digoxigenin linker (Ready-to-Use) 

Ready-to-use linker for ISH applications
Purified IgG fraction of rabbit serum., ISH (in situ hybridization) | Print as PDF
 
ENZ-ABS303-6000 6 ml 426.00 USD
Do you need bulk/larger quantities?
 

Visualize



AMPIVIEW™ RNA Probes can be visualized and imaged with a conventional light microscope.

HIGHDEF® DAB Chromogen/Substrate Set 

Brown chromogen for use with HRP immunohistochemistry staining systems
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC105-0200 200 ml Inquire for pricing
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HIGHDEF® Green AP Chromogen/Substrate 

High definition IHC chromogen producing brilliant green color for AP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC130-0030 30 ml Inquire for pricing
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HIGHDEF® black IHC chromogen (HRP) 

Unique high definition black IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-171-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® blue IHC chromogen (AP) 

High definition IHC chromogen producing brilliant blue color for AP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-150-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® blue IHC chromogen (HRP) 

Unique high definition blue IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-151-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® red IHC chromogen (AP) 

High definition IHC chromogen producing brilliant red color for AP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-140-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® yellow IHC chromogen (HRP) 

Unique high definition yellow IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-170-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® Hematoxylin 

Ready-to-use histologic stain with enhancers
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC106-0100 100 ml Inquire for pricing
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HIGHDEF® IHC mount 

Permanent, high stability mounting medium for high definition visualization of IHC.
IF, IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-261-0030 30 ml Inquire for pricing
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The value of in situ detection of SARS-CoV-2 RNA and proteins in understanding the pathophysiology of severe COVID-19

Understanding the histopathology of COVID-19 has proven to be a major gateway to understanding the molecular mechanisms through which SARS-CoV-2 manifests disease. The group of Dr. Nuovo has contributed significantly to understanding the pathophysiology of severe COVID-19 and utilized Enzo’s AMPIVIEW SARS-CoV-2 RNA probes kit in the process. He will present the highlights of his group’s findings in Enzo’s webinar series. Amongst them: 1. Detection of SARS-CoV-2 RNA in situ shows that productive infection predominates in the nasopharynx and lung; 2. Detection of SARS-CoV-2 proteins (nucleocapsid, spike, envelope and matrix) show that spike is avidly endocytosed via the vascular system in several sites, including the brain and heart; viral RNA is rarely evident in these sites; 3. Correlation of the host response to the in situ localization of SARS-CoV-2 RNA and spike protein shows that each are associated with increased expression of proinflammatory proteins including TNFa and IL6 as well as complement activation.

Main Target Audience: Pathophysiologists; Clinical Laboratories; Hospitals; Histologists; Virologists - Researchers interested in the following topics: Coronavirus, in situ Hybridization (ISH); Antibodies; Clinical Diagnostics; DNA/Protein Labeling, HPV, Immunohistochemistry (IHC), Immunology, Inflammation, Pathology, Virology.

Why should you attend?: Whether you are a scientist, a student, a lab technician, or a curious citizen, this webinar will provide fascinating mechanistic insight into the pathophysiology of severe COVID-19, while simultaneously providing a wealth of practical knowledge on the laboratory technologies utilized in detecting viral RNA and proteins, especially in situ hybridisation and immunohistochemistry.

Presented by: Dr. Gerard J. Nuovo
Professor of Pathology
Ohio State University Comprehensive Cancer Center

Topic: Clinical



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