Based on IEP and/or ELISA, reacts with whole molecule rabbit IgG, and also the light chains of other rabbit immunoglobulins. No antibody detected against non-immunoglobulin serum proteins. Antibody tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins.
Applications:
ELISA, WB
Recommended Dilutions/Conditions:
ELISA (1:2,500-25,000) Western Blot (1:2500-25,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:
Immunoaffinity purified.
Quality Control:
Goat antiserum immunoaffintity purified to isolate specific anti-rabbit IgG antibodies then adsorbed with human IgG and conjugated to alkaline phosphatase.
Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Wound Healing Effects of Rose Placenta in a Mouse Model of Full-Thickness Wounds: Y.W. Kim, et al.; Arch. Plast. Surg. 42, 686 (2015), Application(s): Cell culture, Abstract; Full Text
17beta-estradiol decreases hypoxic induction of erythropoietin gene expression: N.L. Kanagy, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 283, R496 (2002), Application(s): IHC using rat samples, Abstract;