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Goat anti-rabbit IgG, polyclonal antibody (HRP conjugate)

ADI-SAB-300-J 1 ml 127.00 USD
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Product Specification

Immunogen:Purified rabbit IgG (whole molecule).
Species reactivity:Rabbit
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (1:2,500-50,000)
Immunohistochemistry (1:250-2500, Western Blot (1:5,000-100,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Immunoaffinity purified.
Quality Control:The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.
Formulation:Liquid. In PBS, pH 7.6, containing 7.5mg/mL BSA (IgG-free, protease-free) and 50% glycerol.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.
Regulatory Status:RUO - Research Use Only

Product Literature References

Betulinic Acid Inhibits RANKL-Induced Osteoclastogenesis via Attenuating Akt, NF-κB, and PLCγ2-Ca2+ Signaling and Prevents Inflammatory Bone Loss: D.H. Jeong, et al.; J. Nat. Prod. 83, 1174 (2020), Abstract; Full Text
Deubiquitinase USP47-stabilized splicing factor IK regulates the splicing of ATM pre-mRNA: H.I. Ka, et al.; Cell Death Dis. 6, 34 (2020), Abstract; Full Text
Downregulation of c‑FLIP and upregulation of DR‑5 by cantharidin sensitizes TRAIL‑mediated apoptosis in prostate cancer cells via autophagy flux: U.M. Mazim, et al.; Int. J. Mol. Med. 46, 280 (2020), Abstract;
Neferine treatment enhances the TRAIL‑induced apoptosis of human prostate cancer cells via autophagic flux and the JNK pathway: U.M. Mazim, et al.; Int. J. Oncol. 56, 1152 (2020), Abstract; Full Text
AGR2 is a target of canonical Wnt/β-catenin signaling and is important for stemness maintenance in colorectal cancer stem cells: B. Dahal Lamichane, et al.; Biochem. Biophys. Res. Commun. 515, 600 (2019), Abstract;
Essential oil from Korean Chamaecyparis obtusa leaf ameliorates respiratory activity in Sprague‑Dawley rats and exhibits protection from NF-κB-induced inflammation in WI38 fibroblast cells: S. Raha, et al.; Int. J. Mol. Med. 43, 393 (2019), Abstract; Full Text
Perivascular Cells and NADPH Oxidase Inhibition Partially Restore Hyperglycemia-Induced Alterations in Hematopoietic Stem Cell and Myeloid-Derived Suppressor Cell Populations in the Bone Marrow: J.Y. Kim, et al.; Int. J. Stem Cells 12, 63 (2019), Abstract; Full Text
Production of Inflammatory Cytokines and Nitric Oxide by Human Mast Cells Incubated with Toxoplasma gondii Lysate: E. A. Park, et al.; Korean J. Parasitol. 57, 201 (2019), Abstract; Full Text
Rap1 regulates hepatic stellate cell migration through the modulation of RhoA activity in response to TGF-β1: M.Y. Moon, et al.; Int. J. Mol. Med. 44, 491 (2019), Abstract; Full Text
Rosuvastatin attenuates bioprosthetic heart valve calcification: S. Lee, et al.; J. Thorac. Cardiovasc. Surg. 158, 731 (2019), Abstract;
Set7 Is a H3K37 Methyltransferase in Schizosaccharomyces pombe and Is Required for Proper Gametogenesis: Y. Shen, et al.; Structure 27, 631 (2019), Abstract;
The activation of retinal HCA2 receptors by systemic beta-hydroxybutyrate inhibits diabetic retinal damage through reduction of endoplasmic reticulum stress and the NLRP3 inflammasome: M.C. Trotta, et al.; PLoS One 14, e0211005 (2019), Abstract; Full Text
Transgenic overexpression of human LY6K in mice suppresses mature T cell development in the thymus: D. Son, et al.; Oncol. Lett. 17, 379 (2019), Abstract; Full Text
Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse: S. Wang, et al.; Biomaterials 219, 119375 (2019), Abstract;
An Aqueous Extract of Herbal Medicine ALWPs Enhances Cognitive Performance and Inhibits LPS-Induced Neuroinflammation via FAK/NF-κB Signaling Pathways: J.Y. Lee, et al.; Front. Aging Neurosci. 10, 269 (2018), Abstract; Full Text
Anticancer and radiosensitizing effects of the cyclin-dependent kinase inhibitors, AT7519 and SNS‑032, on cervical cancer: M.S. Kang, et al.; Int. J. Oncol. 53, 703 (2018), Abstract;
Combined therapy involving electroacupuncture and treadmillexercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia: M.E. Pak, et al.; Exp Neurol. 300, 222 (2018), Application(s): Western Blot, Abstract;
Defensive Properties of Ginsenoside Re against UV-B-Induced Oxidative Stress through Up-Regulating Glutathione and Superoxide Dismutase in HaCaT Keratinocytes: D. Shin, et al.; Iran. J. Pharm. Res. 17, 249 (2018), Abstract; Full Text
Elevated expression of pancreatic adenocarcinoma upregulated factor (PAUF) is associated with poor prognosis and chemoresistance in epithelial ovarian cancer: C.H. Choi, et al.; Sci. Rep. 8, 12161 (2018), Abstract; Full Text
Inhibition of aldose-reductase-2 by a benzofuroxane derivative bf-5m increases the expression of kcne1, kcnq1 in high glucose cultured H9c2 cardiac cells and sudden cardiac death: M.C. Trotta, et al.; Oncotarget 9, 17257 (2018), Abstract; Full Text
Osteogenic potential of cell spheroids composed of varying ratios of gingiva-derived and bone marrow stem cells using concave microwells: J.Y. Tae, et al.; Exp. Ther. Med. 16, 2287 (2018), Abstract; Full Text
Osterix regulates corticalization for longitudinal bone growth via integrin β3 expression: Y.J. Moon, et al.; Exp. Mol. Med. 50, 80 (2018), Abstract; Full Text
Priming stem cells with protein kinase C activator enhances early stem cell-chondrocyte interaction by increasing adhesion molecules: D. Chae, et al.; Biol. Res. 51, 41 (2018), Abstract; Full Text
Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes: D. Shin, et al.; BMC Complement. Altern. Med. 18, 196 (2018), Abstract; Full Text
Proliferation of prostate epithelia induced by IL-6 from stroma reacted with Trichomonas vaginalis: J.H. Kim, et al.; Parasite Immunol. 40, e12531 (2018), Abstract;
Protective properties of geniposide against UV-B-induced photooxidative stress in human dermal fibroblasts: D. Shin, et al.; Pharm. Biol. 56, 176 (2018), Abstract; Full Text
Resveratrol induces mitochondrial apoptosis and inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma cells: S.E. Kim, et al.; Nutr. Cancer 70, 125 (2018), Abstract;
Resveratrol-mediated apoptosis in renal cell carcinoma via the p53/AMP‑activated protein kinase/mammalian target of rapamycin autophagy signaling pathway: Q. Liu, et al.; Mol. Med. Rep. 17, 502 (2018), Abstract;
The role of AP-1 in self-sufficient proliferation and migration of cancer cells and its potential impact on an autocrine/paracrine loop: S.A.E. Ibrahim, et al.; Oncotarget 9, 34259 (2018), Abstract; Full Text
Tristetraprolin activation by resveratrol inhibits the proliferation and metastasis of colorectal cancer cells: S.R. Lee, et al.; Int. J. Oncol. 53, 1269 (2018), Abstract;
Analysis of endogenous lipids during intestinal wound healing: Y. Lee, et al.; PLoS One 12, e0183028 (2017), Application(s): Western Blot, Abstract; Full Text
cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice: X. Gui, et al.; Mol. Med. Rep. 15, 221 (2017), Abstract; Full Text
Fluid shear stress regulates vascular remodeling via VEGFR-3 activation, although independently of its ligand, VEGF-C, in the uterus during pregnancy: Y.G. Park, et al.; Int. J. Mol. Med. 40, 1210 (2017), Abstract; Full Text
Ginsenoside Rc protects against UVB‑induced photooxidative damage in epidermal keratinocytes: Y. Oh, et al.; Mol. Med. Rep. 16, 2907 (2017), Abstract;
Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover: J.C. Capaz, et al.; Front. Physiol. 8, 344 (2017), Abstract; Full Text
Inhibitory effect of brassinin on TNF‑α‑induced vascular inflammation in human umbilical vein endothelial cells: B.H. Han, et al.; Mol. Med. Rep. 16, 6890 (2017), Abstract;
Korean Scutellaria baicalensis Georgi flavonoid extract induces mitochondrially mediated apoptosis in human gastric cancer AGS cells: V. Ventatarame Gowda Saralamma, et al.; Oncol. Lett. 14, 607 (2017), Abstract; Full Text
Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array: S. Yumnam, et al.; Mol. Med. Rep. 16, 5386 (2017), Abstract; Full Text
Quantification of heat shock protein 70 and acetylcholinesterase over a time course suggests environmental adaptation in a foundational molluscan species: A. Ravaschiere, et al.; Ecotoxicol. Environ. Saf. 142, 222 (2017), Abstract;
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones 22, 225 (2017), Application(s): Western Blot, Abstract; Full Text
Wiryeongtang regulates hypertonicity-induced expression of aquaporin-2 water channels in mIMCD-3 cells: M.G. Kim, et al.; Mol. Med. Rep. 15, 2665 (2017), Abstract;
Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line: Y. Oh, et al.; J. Photochem. Photobiol. B 163, 170 (2016), Application(s): Western blot, detect proMMP-2 and -9 proteins in cellular lysate, Abstract;
Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane: M. Amarasiri, et al.; Water Res. 95, 383 (2016), Abstract;
Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats: M. Ram, et al.; Int. Immunopharmacol. 30, 137 (2016), Application(s): Western blot, Abstract;
Bottom-up proteomics suggests an association between differential expression of mitochondrial proteins and chronic fatigue syndrome: F. Ciregia, et al.; Transl. Psychiatry 6, e904 (2016), Application(s): Western blot, Abstract;
Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1: H.S. Seol, et al.; Cancer Lett. 372, 24 (2016), Abstract; Full Text
Contribution of ginsenoside Re to cellular redox homeostasis via upregulating glutathione and superoxide dismutase in HaCaT keratinocytes under normal conditions: H.W. Lim, et al.; Pharmazie 71, 413 (2016), Abstract;
Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts: B. Beomyeol, et al.; Korean J. Physiol. Pharmacol. 20, 269 (2016), Application(s): Western blot, Abstract; Full Text
Hypoxia‑mediated activation of autophagic flux inhibits apoptosis of keratinocytes via blocking tumor necrosis factor‑related apoptosis‑inducing ligand: S.W. Kim, et al.; Mol. Med. Rep. 13, 805 (2016), Abstract;
NEB-related core-rod myopathy with distinct clinical and pathological features: Y.E. Park, et al.; Muscle Nerve 53, 479 (2016), Abstract;
Pass the salt: physiological consequences of ecologically relevant hyposmotic exposure in juvenile gummy sharks (Mustelus antarcticus) and school sharks (Galeorhinus galeus): A.J. Morash, et al.; Conserv. Physiol. 4, cow036 (2016), Abstract; Full Text
Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression: H. Nassour, et al.; Sci. Rep. 6, 29389 (2016), Application(s): Antibody - immunofluorescence assay, Abstract; Full Text
Photoprotective properties of 20(S)-protopanaxatriol, an aglycone of ginseng saponins: Protection from ultraviolet-B radiation-induced oxidative stress in human epidermal keratinocytes: S.J. Oh, et al.; Mol. Med. Rep. 14, 2839 (2016), Abstract;
Plasma glutathione reductase activity and prognosis of septic shock: J.S. Kim, et al.; J. Surg. Res. 200, 298 (2016), Application(s): Western Blot, Abstract;
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated With Trichomonas vaginalis via Crosstalk With Mast Cell: J.H. Kim, et al.; Prostate 76, 1431 (2016), Abstract;
Protective effects of a herbal extract combination of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein: Y. Sim, et al.; Neuroscience 340, 166 (2016), Application(s): Western Blot, Abstract;
Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes: C.H. Kim, et al.; Int. J. Nanomed. 11, 2407 (2016), Application(s): Western blot, Abstract; Full Text
Stereospecificity of ginsenoside Rg2 epimers in the protective response against UV-B radiation-induced oxidative stress in human epidermal keratinocytes: H.J. Kang, et al.; J. Photochem. Photobiol. B. 165, 232 (2016), Application(s): Western blot, cell lysate, Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Western blot; evaluation of the role of VD2 on anti-inflammation, Abstract;
A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats: A.A. Fernandes, et al.; Braz. J. Med. Biol. Res. 48, 813 (2015), Application(s): Western Blot, Abstract; Full Text
An Aβ42 uptake and degradation via Rg3 requires an activation of caveolin, clathrin and Aβ-degrading enzymes in microglia: S. K. Jang, et al.; Eur. J. Pharmacol. 758, 1 (2015), Application(s): Western Blot, Abstract;
Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling: P. Waghe, et al.; Chem. Biol. Interact. 237, 104 (2015), Application(s): Western Blot, Abstract;
Chronic nicotine treatment enhances vascular smooth muscle relaxation in rats: T. Y. Xu, et al.; Acta. Pharmacol. Sin. 36, 429 (2015), Application(s): Chemiluminescence Western Blot, Abstract;
Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβII in Uremic Accelerated Atherosclerosis Patients: W. Wang, et al.; Chin. Med. J. (Engl). 128, 1465 (2015), Application(s): Western Blot, Abstract; Full Text
Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway: G. Dong, et al.; J. Funct. Foods 18, 47 (2015), Application(s): Immunoprecipitation,
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice: S.J. Woo, et al.; J. Nutr. Biochem. 26, 713 (2015), Application(s): Western Blotting, Abstract;
Host responses associated with chronic staphylococcal mastitis in rabbits: I. Guerrero, et al.; Vet. J. 204, 338 (2015), Application(s): Immunohistochemistry, Abstract;
Housing temperature-induced stress drives therapeutic resistance in murine tumour models through β2-adrenergic receptor activation: J.W. Eng, et al.; Nat. Commun. 6, 6426 (2015), Application(s): Western Blotting, Abstract;
Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest: S.J. Park, et al.; Am. J. Cancer Res. 5, 2127 (2015), Abstract; Full Text
Mori Fructus improves cognitive and neuronal dysfunction induced by beta-amyloid toxicity through the GSK-3β pathway in vitro and in vivo: H.G. Kim, et al.; J. Ethnopharmacol. 171, 196 (2015), Application(s): Immunocytochemistry and immunohistochemistry, Abstract;
SERCA-2a is Involved in the Right Ventricular Function Following Myocardial Infarction in Rats: A.A. Fernandes, et al.; Life Sci. 124, 24 (2015), Application(s): Western Blotting, Abstract;
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One 10, e0115334 (2015), Application(s): Western Blotting, Abstract; Full Text
Functional variants of human APE1 rescue the DNA repair defects of the yeast AP endonuclease/3'-diesterase-deficient strain: Z. Wang, et al.; DNA Repair (Amst) 22C, 53 (2014), Abstract;
Mechanisms responsible for the acceleration of pulmonary V'O2 on-kinetics in humans after prolonged endurance training: J.A. Zoladz, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 307, R1101 (2014), Abstract;
Skin anti-photoaging properties of ginsenoside Rh2 epimers in UV-B-irradiated human keratinocyte cells: S.J. Oh, et al.; J. Biosci. 39, 673 (2014), Application(s): WB using human cell supernatant, Abstract;
Protein kinase C activation stimulates mesenchymal stem cell adhesion through activation of focal adhesion kinase: B.W. Song, et al.; Cell Transplant. 22, 797 (2013), Application(s): WB using rat cell lysates, Abstract;
Sublethal effects of silver nanoparticles and dissolved silver in freshwater mussels: F. Gagné, et al.; J. Toxicol. Environ. Health A 76, 479 (2013), Abstract;
Induction of Heme Oxygenase-1 and Ferritin in the Kidney in Warm Antibody Hemolytic Anemia: F. Fervenza, et al. ; Am. J. Kidney Dis. 52, 972 (2008), Application(s): IHC using human tissue, Abstract;
Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells: T. Acosta, et al. ; J. Endocrinol. 199, 425 (2008), Application(s): WB using rabbit cell lysates, Abstract;
NAD(P)H oxidase modulates angiogenesis and the development of portosystemic collaterals and splanchnic hyperaemia in portal hypertensive rats: B. Angermayr, et al.; Gut 56, 560 (2007), Application(s): WB using rat tissue, Abstract;
Intracellular delivery of HSP70 using HIV-1 Tat protein transduction domain: H.R. Wong, et al. ; Biochem. Biophys. Res. Commun. 301, 54 (2003), Application(s): WB using mouse samples, Abstract;
Involvement of heme oxygenase-1 (HO-1) in the adaptive protection of human lymphocytes after hyperbaric oxygen (HBO) treatment: G. Speit, et al. ; Carcinogenesis 22, 1979 (2001), Application(s): WB using human samples, Abstract;
p38 MAPK mediates renal tubular cell TNF-alpha production and TNF-alpha-dependent apoptosis during simulated ischemia: M.A. Kaefer, et al. ; Am. J. Physiol. Cell Physiol. 281, C563 (2001), Application(s): WB using porcine samples, Abstract;

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