Product Details
Host: | Rabbit |
|
Immunogen: | Purified rat IgG . |
|
Species reactivity: | Rat
|
|
Specificity: | Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. |
|
Applications: | ELISA, IHC, WB
|
|
Recommended Dilutions/Conditions: | ELISA (1:2,500-50,000) Immunohistochemistry (1:250-2500) Western Blot (1:5,000-100,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Purity Detail: | Immunoaffinity purified. |
|
Quality Control: | The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species. |
|
Formulation: | Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/mL BSA (IgG-free, protease-free), and 50% glycerol. |
|
Shipping: | Blue Ice |
|
Long Term Storage: | -20°C |
|
Scientific Background: | Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG. |
|
Regulatory Status: | RUO - Research Use Only |
|
Product Literature References
Dynein light chain LC8 alleviates nonalcoholic steatohepatitis by inhibiting NF-κB signaling and reducing oxidative stress: G.R. Lee, et al.; J. Cell. Physiol.
237, 3554 (2022),
Abstract;
Analysis of mucosal stress response in acid-induced esophagitis in opossum: W.G. Paterson, et al. ; Dig. Dis. Sci.
47, 1873 (2002),
Application(s): WB using opossum samples,
Abstract;
Nitric oxide (NO) induces nitration of protein kinase Cepsilon (PKC epsilon), facilitating PKC epsilon translocation via enhanced PKC epsilon -RACK2 interactions: a novel mechanism of no-triggered activation of PKC epsilon: P. Ping, et al. ; J. Biol. Chem.
277, 15021 (2002),
Application(s): WB using human & rabbit samples,
Abstract;
Related Products