Host: | Goat |
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Immunogen: | Purified rat IgM (whole molecule). |
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Species reactivity: | Rat
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Specificity: | Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of rat IgM but not with rat IgG or the light chains of rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. |
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Applications: | ELISA, WB
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Recommended Dilutions/Conditions: | ELISA (1:2,500-50,000) Western Blot (1:5,000-100,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Immunoaffinity purified. |
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Quality Control: | The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human, bovine, and horse serum proteins, but it may cross-react with IgM from other species. |
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Formulation: | Liquid. In PBS, pH 7.6, containing 7.5mg/mL BSA and 50% glycerol. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Immunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B-cells as a monomer of two mu heavy chains and two light chains. Following B-cell activation, IgM is secreted in its pentameric form, consisting of five IgM molecules joined at the base of the Fc domain by a J chain. IgM is the first Immunoglobulin expressed following infection. |
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Regulatory Status: | RUO - Research Use Only |
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