Product Details
| Host: | Goat |
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| Immunogen: | Fab fragment of mouse IgG. |
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| Species reactivity: | Mouse
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| Specificity: | Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab’)2/Fab portion of mouse IgG, and also with the light chains of other mouse immunoglobulins. No antibody was detected against the Fc portion of mouse IgG or against other non-immunoglobulin serum proteins. |
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| Applications: | ELISA, IHC, WB
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| Recommended Dilutions/Conditions: | ELISA (1:2,500-1:50,000)
Immunohistochemistry (1:250-1:2500)
Western Blot (1:2500-1:50,000 colorimetric, 1:5,000-1:100,000 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Immunoaffinity purified. |
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| Quality Control: | The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins. |
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| Formulation: | Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/ml BSA (IgG-free, protease-free), and 50% glycerol. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG. |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Enhanced neuronal activity by suffruticosol A extracted from Paeonia lactiflora via partly BDNF signaling in scopolamine-induced memory-impaired mice: J.H. Kim, et al.; Sci. Rep.
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Galectin-4 increases the ability of M2 macrophages to enhance antiviral CD4+ T-cell responses : I.G. Lee, et al.; J. Leukoc. Biol.
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Synergistic antidepressant-like effect of n-3 polyunsaturated fatty acids and probiotics through the brain-gut axis in rats exposed to chronic mild stress: H. Cho, et al.; J. Nutr. Biochem.
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The spleen is the major site for the development and expansion of inhibitor producing-cells in hemophilia A mice upon FVIII infusion developing high-titer inhibitor: A. Oda, et al.; Thromb. Res.
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Anti-fatigue potential of Pinus koraiensis leaf extract in an acute exercise-treated mouse model: S.M. Lee, et al.; Biomed. Pharmacother.
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Bamboo Shoot and Artemisia capillaris Extract Mixture Ameliorates Dextran Sodium Sulfate-Induced Colitis: H.J. Kim, et al.; Curr. Issues Mol. Biol.
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Benefits of a Skull-Interfaced Flexible and Implantable Multilight Emitting Diode Array for Photobiomodulation in Ischemic Stroke: H. Kim, et al.; Adv. Sci. (Weinh.)
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CDDO, a PPAR‑γ ligand, inhibits TPA‑induced cell migration and invasion through a PPAR‑γ‑independent mechanism: H.Y. Jang, et al.; Oncol. Lett.
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Data on the evaluation of the relation between β-arrestin 2 and YAP phosphorylation in patient-derived colon cancer organoid: M. Kim, et al.; Data Brief
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Effects of Daehwang-Hwanglyoun-Sasim-Tang on brain injury and cognitive function in mice caused by bilateral common carotid artery stenosis: M.H. Lee, et al.; Int. J. Med. Sci.
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Eriodictyol protects skin cells from UVA irradiation-induced photodamage by inhibition of the MAPK signaling pathway: M.F. Nisar, et al.; J. Photochem. Photobiol. B
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HIV-1 Nef Protein Affects Cytokine and Extracellular Vesicles Production in the GEN2.2 Plasmacytoid Dendritic Cell Line: A. Aiello, et al.; Viruses
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Inhibitory Effect of Etravirine, a Non-Nucleoside Reverse Transcriptase Inhibitor, via Anterior Gradient Protein 2 Homolog Degradation against Ovarian Cancer Metastasis: T.T.G. Ly, et al.; Int. J. Mol. Sci.
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NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma: J. Kim, et al.; Exp. Mol. Med.
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Reduced expression of TAZ inhibits primary cilium formation in renal glomeruli: J.H. Jun, et al.; Exp. Mol. Med.
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The C. elegans regulatory factor X (RFX) DAF-19M module: A shift from general ciliogenesis to cell-specific ciliary and behavioral specialization: S. Ahn, et al.; Cell Rep.
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The loss of epithelial Smad4 drives immune evasion via CXCL1 while displaying vulnerability to combinatorial immunotherapy in gastric cancer: H.W. An, et al.; Cell Rep.
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Betulinic Acid Improves Cardiac-Renal Dysfunction Caused by Hypertrophy through Calcineurin-NFATc3 Signaling: M.H. Hong, et al.; Nutrients
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Central administration of afzelin extracted from Ribes fasciculatum improves cognitive and memory function in a mouse model of dementia: S.Y. Oh, et al.; Sci. Rep.
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Characterization of subcellular localization of eukaryotic clamp loader/unloader and its regulatory mechanism: S. H. Park, et al.; Sci. Rep.
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Macrophage-Inducible C-Type Lectin Signaling Exacerbates Acetaminophen-Induced Liver Injury by Promoting Kupffer Cell Activation in Mice: J. Zhao, et al.; Mol. Pharmacol.
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PML-II regulates ERK and AKT signal activation and IFNα-induced cell death: X. Meng, et al.; Cell Commun. Signal.
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The Anti-diabetic Drug Gliquidone Modulates Lipopolysaccharide-Mediated Microglial Neuroinflammatory Responses by Inhibiting the NLRP3 Inflammasome: J. Kim, et al.; Front. Aging Neurosci.
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Cinnamaldehyde protects against oxidative stress and inhibits the TNF‑α‑induced inflammatory response in human umbilical vein endothelial cells: N.Y. Kim, et al.; Int. J. Mol. Med.
46, 280 (2020),
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Deubiquitinase USP47-stabilized splicing factor IK regulates the splicing of ATM pre-mRNA: H.I. Ka, et al.; Cell Death Dis.
6, 34 (2020),
Abstract;
Full Text
Downregulation of c‑FLIP and upregulation of DR‑5 by cantharidin sensitizes TRAIL‑mediated apoptosis in prostate cancer cells via autophagy flux: U.M. Mazim, et al.; Int. J. Mol. Med.
46, 280 (2020),
Abstract;
Harnessing adipose‑derived stem cells to release specialized secretome for the treatment of hepatitis B: H. Kim, et al.; Int. J. Mol. Med.
47, 15 (2020),
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Inhibitory effects on melanogenesis by thymoquinone are mediated through the β‑catenin pathway in B16F10 mouse melanoma cells: H. Jeong, et al.; Int. J. Oncol.
56, 379 (2020),
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Neferine treatment enhances the TRAIL‑induced apoptosis of human prostate cancer cells via autophagic flux and the JNK pathway: U.M. Mazim, et al.; Int. J. Oncol.
56, 1152 (2020),
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Full Text
AGR2 is a target of canonical Wnt/β-catenin signaling and is important for stemness maintenance in colorectal cancer stem cells: B. Dahal Lamichane, et al.; Biochem. Biophys. Res. Commun.
515, 600 (2019),
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Rap1 regulates hepatic stellate cell migration through the modulation of RhoA activity in response to TGF-β2: M.Y. Moon, et al.; Int. J. Mol. Med.
44, 491 (2019),
Abstract;
Full Text
The activation of retinal HCA2 receptors by systemic beta-hydroxybutyrate inhibits diabetic retinal damage through reduction of endoplasmic reticulum stress and the NLRP3 inflammasome: M.C. Trotta, et al.; PLoS One
14, e0211005 (2019),
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Full Text
Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse: S. Wang, et al.; Biomaterials
219, 119375 (2019),
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An Aqueous Extract of Herbal Medicine ALWPs Enhances Cognitive Performance and Inhibits LPS-Induced Neuroinflammation via FAK/NF-κB Signaling Pathways: J.Y. Lee, et al.; Front. Aging Neurosci.
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Full Text
Combined therapy involving electroacupuncture and treadmill exercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia: M.E. Pak, et al.; Exp. Neurol.
300, 222 (2018),
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Combined therapy involving electroacupuncture and treadmillexercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia: M.E. Pak, et al.; Exp.Neurol.
300, 222 (2018),
Application(s): Western Blot,
Abstract;
Identification and characterization of site-specific N-glycosylation in the potassium channel Kv3.1b: P.C. Vicente, et al.; J. Cell. Physiol.
233, 549 (2018),
Abstract;
Osteogenic potential of cell spheroids composed of varying ratios of gingiva-derived and bone marrow stem cells using concave microwells: J.Y. Tae, et al.; Exp. Ther. Med.
16, 2287 (2018),
Abstract;
Full Text
PEP-1-glutaredoxin-1 induces dedifferentiation of rabbit articular chondrocytes by the endoplasmic reticulum stress-dependent ERK-1/2 pathway and the endoplasmic reticulum stress-independent p38 kinase and PI-3 kinase pathways: S.M. Yu, et al.; Int. J. Biol. Macromol.
111, 1059 (2018),
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Simvastatin-dependent actin cytoskeleton rearrangement regulates differentiation via the extracellular signal-regulated kinase-1/2 and p38 kinase pathways in rabbit articular chondrocytes: Y. Han, et al.; Eur. J. Pharmacol.
834, 197 (2018),
Abstract;
Analysis of endogenous lipids during intestinal wound healing: Y. Lee, et al.; PLoS One
12, e0183028 (2017),
Application(s): Western Blot,
Abstract;
Full Text
cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice: X. Gui, et al.; Mol. Med. Rep.
15, 221 (2017),
Abstract;
Full Text
Fluid shear stress regulates vascular remodeling via VEGFR-3 activation, although independently of its ligand, VEGF-C, in the uterus during pregnancy: Y.G. Park, et al.; Int. J. Mol. Med.
40, 1210 (2017),
Abstract;
Full Text
Korean Scutellaria baicalensis Georgi flavonoid extract induces mitochondrially mediated apoptosis in human gastric cancer AGS cells: V. Ventatarame Gowda Saralamma, et al.; Oncol. Lett.
14, 607 (2017),
Abstract;
Full Text
Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array: S. Yumnam, et al.; Mol. Med. Rep.
16, 5386 (2017),
Abstract;
Full Text
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones
22, 225 (2017),
Application(s): Western Blot,
Abstract;
Full Text
Berberine induces dedifferentiation by actin cytoskeleton reorganization via phosphoinositide 3-kinase/Akt and p38 kinase pathways in rabbit articular chondrocytes: S.M. Yu, et al.; Exp. Biol. Med.
241, 800 (2016),
Abstract;
Full Text
Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1: H.S. Seol, et al.; Cancer Lett.
372, 24 (2016),
Abstract;
Full Text
Cytokine-induced apoptosis inhibitor-1 causes dedifferentiation of rabbit articular chondrocytes via the ERK-1/2 and p38 kinase pathways: S.M. Yu, et al.; Int. J. Biochem. Cell Biol.
80, 10 (2016),
Abstract;
Erythropoietin and a nonerythropoietic peptide analog promote aortic endothelial cell repair under hypoxic conditions: role of nitric oxide: L. Heikal, et al.; Hypoxia
4, 121 (2016),
Abstract;
Full Text
Hypoxia‑mediated activation of autophagic flux inhibits apoptosis of keratinocytes via blocking tumor necrosis factor‑related apoptosis‑inducing ligand: S.W. Kim, et al.; Mol. Med. Rep.
13, 805 (2016),
Abstract;
Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression: H. Nassour, et al.; Sci. Rep.
6, 29389 (2016),
Application(s): Antibody - immunofluorescence assay,
Abstract;
Full Text
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated With Trichomonas vaginalis via Crosstalk With Mast Cell: J.H. Kim, et al.; Prostate
76, 1431 (2016),
Abstract;
Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes: C.H. Kim, et al.; Int. J. Nanomed.
11, 2407 (2016),
Application(s): Western blot,
Abstract;
Full Text
Simvastatin induces differentiation of rabbit articular chondrocytes via the ERK-1/2 and p38 kinase pathways: Y. Han, et al.; Exp. Cell Res.
346, 198 (2016),
Application(s): Immunohistochemistry,
Abstract;
A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats: A.A. Fernandes, et al.; Braz. J. Med. Biol. Res.
48, 813 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβII in Uremic Accelerated Atherosclerosis Patients: W. Wang, et al.; Chin. Med. J. (Engl).
128, 1465 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway: G. Dong, et al.; J. Funct. Foods 18, 47 (2015), Application(s): Immunoprecipitation,
Genomic and proteomic investigation of preeclampsia: H. Sahin, et al.; Exp. Ther. Med.
10, 711 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Heme Oxygenase-1 Protects Endothelial Cells from the Toxicity of Air Pollutant Chemicals: A. Lawal, et al.; Toxicol. Appl. Pharmacol.
284, 281 (2015),
Application(s): Western Blotting,
Abstract;
Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest: S.J. Park, et al.; Am. J. Cancer Res.
5, 2127 (2015),
Abstract;
Full Text
Molecular Characterization and expression analysis of hsp60 gene homologue of Sheep Blowfly, Lucilia cuprina: M.K. Singh, et al.; J. Therm. Biol.
52, 24 (2015),
Application(s): Western Blot,
Abstract;
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One
10, e0115334 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
[6]-Gingerol Affects Glucose Metabolism by Dual Regulation via the AMPKα2-Mediated AS160-Rab5 Pathway and AMPK-Mediated Insulin Sensitizing Effects: J.O. Lee, et al.; J. Cell. Biochem.
116, 1401 (2015),
Abstract;
Src regulates membrane trafficking of the Kv3.1b channel: S.H. Bae, et al.; FEBS Lett.
588, 86 (2014),
Abstract;
Low mercury concentrations cause oxidative stress and endothelial dysfunction in conductance and resistance arteries: M. Salaices, et al. ; Am. J. Physiol. Heart Circ. Physiol.
295, H1033 (2008),
Application(s): WB using rat tissue,
Abstract;
Persistent sub-lethal chlorine exposure augments temperature induced immunosuppression in Cyprinus carpio advanced fingerlings: A. Pal, et al. ; Fish Shellfish Immunol.
22, 547 (2007),
Application(s): WB using fish samples,
Abstract;
Oxidative stress and induction of heme oxygenase-1 in the kidney in sickle cell disease: R.P. Hebbel, et al. ; Am. J. Pathol.
158, 893 (2001),
Application(s): IHC using human samples,
Abstract;
Zinc metallothionein imported into liver mitochondria modulates respiration: B.L. Vallee, et al. ; PNAS
98, 2317 (2001),
Application(s): WB using rat samples,
Abstract;
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