Immunohistochemistry (paraffin sections 1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~25kDa by Western blot.
Purity Detail:
Protein A/G-affinity purified.
Formulation:
Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide.
Shipping:
Blue Ice Not Frozen
Short Term Storage:
+4°C
Scientific Background:
Mouse neutrophil gelatinase-associated lipocalin (NGAL; also lipocalin 2, 24p3, SIP24, uterocalin and siderocalin) belongs to the lipocalin family of proteins which bind and transport small lipophilic molecules. Following research showing 24p3 dramatically upregulated in kidney cell cultures infected with SV40, it was subsequently identified as a lipocalin. Unlike human NGAL, mouse NGAL is an acute phase protein upregulated in the liver, but like its mammalian homologues, it is upregulated and secreted by the kidney in response to ischemic or nephrotoxic injury. It is also upregulated in the involuting uterus and mammary gland.
Regulatory Status:
RUO - Research Use Only
NGAL expression 2 days after introduction of ischemic-reperfusion injured mouse kidney; detected with anti-mouse NGAL antibody (ABS 043-29; antibody dilution 1:1000). Image and data supplied by Prof. Güler, Medizinische Hochschule Hannover.
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General Literature References
Application of emerging biomarkers of acute kidney injury in development of kidney-sparing polypeptide-based antibiotics: D. Burt, et al.; Drug Chem. Toxicol. 37, 204 (2014), Abstract;
Ultra sensitive (1.279 pg/ml) ELISA kit, enabling reduced input sample and matrix interference, for the quantification of KIM-1, an early biomarker for kidney injury or disease.