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Creatinine colorimetric detection kit

Low price, rapid creatinine detection kit for diabetes, cardiovascular and renal research.
ADI-907-030A 2x96 wells 267.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: ADI-907-030

  • Easy-to-use one-step creatinine detection
  • Fast homogeneous mix-and-ready assay with no wash steps, results in just 30 minutes
  • Accurate and calibrated to NIST creatinine standard
The Creatinine colorimetric detection kit utilizes a single-step liquid detection reagent that is safer and less time consuming than other assay methods. This kit is calibrated against the NIST standard and offers reproducible results with less than 6% inter- and intra-assay variation.
Creatinine colorimetric detection kit image
Creatinine colorimetric detection kit Standard curve
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Creatinine colorimetric detection kit image Creatinine colorimetric detection kit Standard curve

Product Details

Sensitivity:0.042 mg/dl (range 0.31 - 20 mg/dl)
Assay Time:30 minutes
Applications:Colorimetric detection
Application Notes:For the quantitative determination of urinary creatinine from any species.
Species reactivity:Species independent
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Contents:Microtiter plate, Standard, Detection Reagent
Scientific Background:Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from p-creatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Discriminating Different Bladder and Bladder Outlet Dysfunctions by Urinary Biomarkers in Women with Frequency–Urgency Syndrome: J.F. Jhang, et al.; Biomedicines 11, 673 (2023), Abstract;
The Effect of Acute Aerobic Exercise on Biomarkers of Renal Health and Filtration in Moderate-CKD: J.S. Forsse, et al.; Res. Q. Exerc. Sport (2023), Abstract;
Effect of Age and Acute-Moderate Intensity Exercise on Biomarkers of Renal Health and Filtration: J.S. Forsse, et al.; Biology 11, 527 (2022), Abstract;
Improving correlation of wastewater SARS-CoV-2 gene copy numbers with COVID-19 public health cases using readily available biomarkers: J.M. Hutchison, et al.; FEMS Microbes 3, xtac010 (2022), Abstract;
Pexophagy is critical for fungal development, stress response, and virulence in Alternaria alternata: P.C. Wu, et al.; Mol. Plant. Pathol. 23, 1538 (2022), Abstract;
Platelet thromboxane inhibition by low-dose aspirin in polycythemia vera: Ex vivo and in vivo measurements and in silico simulation: G. Petrucci, et al.; Clin. Transl. Sci. 15, 2958 (2022), Abstract;
Role of urinary H2O2, 8-iso-PGF2α, and serum oxLDL/β2GP1 complex in the diabetic kidney disease: R. Sauriasari, et al.; PLoS One 17, e0263113 (2022), Abstract;
Urinary neopterin: A novel biomarker of disease progression in amyotrophic lateral sclerosis: S.R. Shepheard, et al.; Eur. J. Neurol. 29, 990 (2022), Abstract;
Protective Effects of Eicosapentaenoic Acid on the Glomerular Endothelium via Inhibition of EndMT in Diabetes: T. Yasuzawa, et al.; J. Diabetes Res. 2021, 2182225 (2021), Abstract;
Scavenging of reactive dicarbonyls with 2-hydroxybenzylamine reduces atherosclerosis in hypercholesterolemic Ldlr−/− mice: H. Tao, et al.; Nat. Commun. 11, 4084 (2020), Application(s): Human Urine, Abstract; Full Text
BCG Therapy of Bladder Cancer Stimulates a Prolonged Release of the Chemoattractant CXCL10 (IP10) in Patient Urine: O. Ashiru, et al.; Cancers (Basel) 11, 940 (2019), Abstract;
Cordyceps militaris Improves Chronic Kidney Disease by Affecting TLR4/NF-κB Redox Signaling Pathway: T. Sun, et al.; Oxid. Med. Cell. Longev. 2019, 7850863 (2019), Abstract; Full Text
In vivo thromboxane-dependent platelet activation is persistently enhanced in subjects with impaired glucose tolerance: F. Santilli, et al.; Diabetes Metab. Res. Rev. 36, e3232 (2019), Application(s): Human Urine, Abstract;
Behavioral immune system activity predicts downregulation of chronic basal inflammation: J. Gassen, et al.; PLoS One 13, e0203961 (2018), Abstract; Full Text
The Correlation Between Urinary 8-Iso-Prostaglandin F2α and Hydrogen Peroxide Toward Renal Function in T2DM Patients Consuming Sulfonylurea and Combination of Metformin-Sulfonylurea: R. Sauriasari, et al.; Curr. Diabetes Rev. 14, 189 (2018), Abstract;
Effect of condensed tannin extract supplementation on growth performance, nitrogen balance, gas emissions, and energetic losses of beef steers: P.J. Ebert, et al.; J. Animal Sci. 95, 1345 (2017), Abstract;
Antiplatelet Effect Durability of a Novel, 24-Hour, Extended-ReleasePrescription Formulation of Acetylsalicylic Acid in Patients With Type 2Diabetes Mellitus: P.A. Gurbel, et al.; Am. J. Cardiol. 118, 1941 (2016), Application(s): Urinary creatinine levels in patients with type 2 diabetes mellitus, Abstract;
In vivo platelet activation and aspirin responsiveness in type 1 diabetes mellitus: F. Zaccardi, et al.; Diabetes 65, 503 (2016), Abstract;
Moderate intake of docosahexaenoic acid raises plasma and platelet vitamin E levels in cystic fibrosis patients: E. Vericel, et al.; Prostaglandins Leukot. Essent. Fatty Acids 115, 41 (2016), Application(s): Urine, Abstract; Full Text
Skatole metabolites in urine as a biological marker of pigs with enhanced hepatic metabolism: C. Brunius, et al.; Animal 10, 1734 (2016), Abstract;
A cisplatin‑incorporated liposome that targets the epidermal growth factor receptor enhances radiotherapeutic efficacy without nephrotoxicity: J. Jung, et al.; Int. J. Oncol. 46, 1268 (2015), Abstract;
Fruit and vegetable intake and urinary levels of prostaglandin E2 metabolite in postmenopausal women: S. Kim, et al.; Nutr. Cancer 67, 580 (2015), Application(s): Creatinine levels in urine, Abstract; Full Text
Moderate oral supplementation with docosahexaenoic acid improves platelet function and oxidative stress in type 2 diabetic patients: E. Véricel, et al.; Thromb. Haemost. 114, 289 (2015), Application(s): Human Urine, Abstract;
Liver but not adipose tissue is responsive to the pattern of enteral feeding: Y.F. Otero, et al.; Physiol. Rep. 2, e00250 (2014), Application(s): Assay using mouse urine, Abstract; Full Text
Urinary biomarkers track the progression of nephropathy in hypertensive and obese rats: Q. Zhang, et al.; Biomark. Med. 8, 85 (2014), Application(s): Creatinine levels in urine, Abstract; Full Text
Mice Do Not Habituate to Metabolism Cage Housing–A Three Week Study of Male BALB/c Mice: O. Kalliokoski, et al.; PLoS One 8, e58460 (2013), Application(s): Assay using mouse urine, Abstract; Full Text
Potential trends in Attention Deficit Hyperactivity Disorder (ADHD) drug use on a college campus: wastewater analysis of amphetamine and ritalinic acid: D.A. Burgard, et al.; Sci. Total Environ. 450, 242 (2013), Application(s): Assay using acidified wastewater, Abstract;
Variation of bowhead whale progesterone concentrations across demographic groups and sample matrices: N.M. Kellar, et al.; Endanger. Species Res. 22, 61 (2013), Application(s): Whale Urine, Full Text

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