Liquid. In 0.01 M phosphate buffer, pH 7.4, containing 0.14 M NaCl, 0.5% BSA, 0.5% Tween 20, 0.0016% Phenol red, and 0.1% Proclin 950.
Applications:
ELISA
Shipping:
Blue Ice Not Frozen
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Scientific Background:
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury.
Regulatory Status:
RUO - Research Use Only
Product Literature References
The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis: Bangert, et al.; J. Am. Soc. Nephrol. 24, 368A (2013),
General Literature References
Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases: Nielsen, et al.; Gut 38, 414 (1996),
Isolation and primary structure of NGAL, a novel protein associated with human
neutrophil gelatinase: Kjeldsen, et al.; J. Biol. Chem. 268, 10425 (1993),
Ultra sensitive (1.279 pg/ml) ELISA kit, enabling reduced input sample and matrix interference, for the quantification of KIM-1, an early biomarker for kidney injury or disease.
