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Goat anti-rat IgM, polyclonal antibody (HRP conjugate)

 
ADI-SAB-210-J 1 ml 129.00 USD
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Product Specification

Host:Goat
 
Immunogen:Purified rat IgM (whole molecule).
 
Species reactivity:Rat
 
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of rat IgM but not with rat IgG or the light chains of rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (1:2,500-50,000)
Western Blot (1:5,000-100,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Quality Control:The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human, bovine, and horse serum proteins, but it may cross-react with IgM from other species.
 
Formulation:Liquid. In PBS, pH 7.6, containing 7.5mg/mL BSA and 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Immunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B-cells as a monomer of two mu heavy chains and two light chains. Following B-cell activation, IgM is secreted in its pentameric form, consisting of five IgM molecules joined at the base of the Fc domain by a J chain. IgM is the first Immunoglobulin expressed following infection.
 

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